sebov-detection

A Survey of Virus Recombination Uncovers Canonical Choices of Artificial Chimeras Generated All through Deep Sequencing Library Preparation.

Chimeric reads shall be generated by in vitro recombination all through the preparation of high-throughput sequencing libraries. Our attempt to detect natural recombination between the genomes of dengue virus (DENV; +ssRNA genome) and its mosquito host using the Illumina Nextera sequencing library preparation bundle revealed that almost all, if not all, detected host-virus chimeras had been artificial.
Actually, these chimeras weren’t further frequent than with administration RNA from one different species (a pillbug), which was certainly not in contact with DENV RNA earlier to the library preparation.
The proportion of chimera varieties merely mirrored these of the three species amongst sequencing reads. Chimeras had been constantly characterised by the presence of 1-20 bp microhomology between recombining fragments. Inside-species chimeras largely involved fragments in reverse orientations and located decrease than 100 bp from each other inside the parental genome.
We found associated choices in revealed datasets using two totally different viruses: Ebola virus (EBOV; -ssRNA genome) and a herpesvirus (dsDNA genome), every produced with the Illumina Nextera protocol. These canonical choices advocate that artificial chimeras are generated by intra-molecular template switching of the DNA polymerase all through the PCR step of the Nextera protocol.
Lastly, a printed Illumina dataset using the Flock House virus (FHV; +ssRNA genome) generated with a protocol stopping artificial recombination revealed the presence of 1-10 bp microhomology motifs in FHV-FHV chimeras, nonetheless only some recombining fragments had been in reverse orientations.
Our analysis uncovered sequence choices characterizing recombination breakpoints in short-read sequencing datasets, which shall be helpful to guage the presence and extent of artificial recombination.
sebov-detection

sebov-detection

Resistin (43-65) (Human) - Antibody

H-028-39 50 μl
EUR 604.8

Resistin (51-108) (Human) - Antibody

H-028-40 50 μl
EUR 604.8

Anti-Human Resistin Antibody

102-P92G 100 µg
EUR 245.7
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis.

Anti-Human Resistin Antibody

101-M170 500 µg
EUR 246.75
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5 kDa disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.

Anti-Human Resistin Antibody

101-M618 100 µg
EUR 399
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis.

Human Resistin (RETN)

1-CSB-EP019573HU
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  • 1MG
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Description: Recombinant Human Resistin(RETN) expressed in E.coli

Biotinylated Anti-Human Resistin

GWB-BIG87D 1 kit Ask for price

Recombinant Human Resistin

7-01519 5µg Ask for price

Recombinant Human Resistin

7-01520 25µg Ask for price

Recombinant Human Resistin

7-01521 1mg Ask for price

RECOMBINANT HUMAN RESISTIN

GWB-F4A137 0.02 mg Ask for price

Recombinant Human Resistin

cyt-456-1mg 1mg
EUR 4680

Recombinant Human Resistin

cyt-456-25g 25µg
EUR 145

Recombinant Human Resistin

cyt-456-5g 5µg
EUR 60

Resistin (43-65) (Human) - RIA Kit

RK-028-39 125 tubes
EUR 1061.64

human Resistin ELISA KIT

201-12-0339 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Resistin ELISA kit

E01A7519 96T
EUR 700
Description: ELISA

Human Resistin ELISA kit

E01R0351-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01R0351-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01R0351-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA Kit

E16HE0035 96T
EUR 833.33

Human Resistin ELISA KIT

E16HE0035-048 48 wells
EUR 295

Human Resistin ELISA KIT

E16HE0035-096 96 wells
EUR 395

Human Resistin ELISA kit

E22-HC136.48 48T
EUR 295

Human Resistin ELISA kit

E22-HC136.96 96T
EUR 395

Human Resistin ELISA Kit

E28L0045 96T
EUR 666.67

Human Resistin ELISA KIT

E42EH-93 96T/48T Ask for price

Human Resistin ELISA KIT

EKC40240-48T 48T
EUR 412.02

Human Resistin ELISA KIT

EKC40240-5x96T 5x96T
EUR 2795.85

Human Resistin ELISA KIT

EKC40240-96T 96T
EUR 588.6

Human Resistin ELISA Kit

EK2351 96 tests
EUR 497

Human Resistin ELISA Kit

EHR0358 96Tests
EUR 625.2

Human Resistin ELISA KIT

CSB-E06884h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Resistin ELISA KIT

1-CSB-E06884h
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  • 1 plate of 96 wells
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  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Resistin ELISA kit

55R-1714 1 kit
EUR 717
Description: ELISA kit for the detection of Human Resistin in the research laboratory

Human Resistin ELISA kit

55R-2127 96 tests
EUR 825
Description: ELISA Kit for detection of Resistin in the research laboratory

Human Resistin ELISA kit

55R-E50 96 wells
EUR 715
Description: ELISA kit for the detection of Human Resistin in the research laboratory

human Resistin ELISA KIT

CN-04165H1 96T
EUR 530.4

human Resistin ELISA KIT

CN-04165H2 48T
EUR 351.6

Human Resistin ELISA kit

LF-EK50441 1×96T
EUR 790.8

Human Resistin ELISA Kit

LF-EK70021 1×96T
EUR 625.2

Human Resistin ELISA Kit

RK00139 96 Tests
EUR 280

Human Resistin ELISA Kit

GWB-SKR059 96 Tests Ask for price

Human Resistin ELISA Kit

GWB-ZZD207 1x96 well plate Ask for price

Human Resistin ELISA Kit

SL1530Hu 96 Tests
EUR 468

Anti- Resistin Mouse Antibody

GWB-11F9B7 0.1 mg Ask for price

Resistin, human recombinant

4559-100 each
EUR 874.8

Resistin, human recombinant

4559-1000 each
EUR 6127.2

Resistin, human recombinant

4559-25 each
EUR 307.2

Resistin (Recombinant) Human

GWB-8EE40A 0.025 mg Ask for price

Resistin, recombinant (Human)

028-36 20 μg
EUR 423.36

Resistin Human Recombinant

rAP-0727 Inquiry Ask for price

Mouse Monoclonal anti-human Resistin

hAP-0528 100ug
EUR 250

Canine Resistin(Resistin) ELISA Kit

QY-E70171 96T
EUR 511.2

Resistin (Human) ELISA Kit

EKA51875-5x96T 5x96T Ask for price

Resistin (Human) ELISA Kit

EKA51875-96T 96T
EUR 446.9

Anti-Mouse Resistin Antibody

103-M461 100 µg
EUR 399
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant murine Resistin is a 20.2 kDa disulfide-linked homodimeric protein composed of two identical 94 amino acid chains linked by a single disulfide bond.

Anti-Mouse Resistin Antibody

103-P51G 100 µg
EUR 245.7
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant murine Resistin is a 20.2 kDa disulfide-linked homodimeric protein composed of two identical 94 amino acid chains linked by a single disulfide bond.

Human Resistin (RETN) Protein

20-abx651562
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  • 100 ug
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  • 1 mg
  • 200 ug
  • 50 ug

Human Resistin (RETN) Protein

abx670217-25ug 25 ug
EUR 661.2

Human Resistin (RETN) Protein

20-abx068871
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  • 100 ug
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Human Resistin (RETN) Protein

abx262050-25mg 2.5 mg
EUR 325

Human Resistin (RETN) Protein

abx262253-10mg 10 mg
EUR 325

Human Resistin (RETN) Protein

abx262253-25mg 25 mg
EUR 5975

Human Resistin (RETN) Protein

abx262253-5mg 5 mg
EUR 225

Human Resistin (RETN) Protein

abx068871-10g 10 µg
EUR 375

Human Resistin (RETN) Protein

abx068871-2g 2 µg
EUR 212.5

Human Resistin (RETN) Protein

abx670217-50g 50 µg
EUR 462.5

Human Resistin (RETN) Protein

abx651562-100g 100 µg
EUR 1062.5

Human Resistin (RETN) Protein

abx651562-10g 10 µg
EUR 587.5

Human Resistin (RETN) Protein

abx651562-50g 50 µg
EUR 875

Human Resistin (RETN) Protein

abx682081-100g 100 µg
EUR 812.5

Human Resistin (RETN) Protein

abx682081-20g 20 µg
EUR 312.5

Resistin (43-65) (Human) - FAM Labeled

FG-028-39A 1 nmol
EUR 604.8

Resistin (51-108) (Human) - Purified IgG Antibody

G-028-40 200 μg
EUR 604.8

Polyclonal Anti- Resistin Antibody

GWB-BBP350 0.1 mg Ask for price

Recombinant Human Resistin, HEK

cyt-1183-10g 10µg
EUR 145

Recombinant Human Resistin, HEK

cyt-1183-1mg 1mg
EUR 5200

Recombinant Human Resistin, HEK

cyt-1183-2g 2µg
EUR 60

Recombinant Human Resistin (RETN)

CSB-EP019573HU 2058 mg Ask for price

Recombinant Human Resistin(RETN)

AP70712 1mg
EUR 1978

Recombinant Human Resistin (RETN)

Z100805 25 µg
EUR 85
Description: Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down-regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target-tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90% in 3T3-L1 adipocytes.

Recombinant Human Resistin (RETN)

Z100809 1.0 mg
EUR 1500
Description: Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down-regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target-tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90% in 3T3-L1 adipocytes.

Human Resistin(REN) Elisa Kit

EK710243 96 Wells
EUR 0.74

Human Resistin (RETN) CLIA Kit

20-abx492128
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Resistin (RETN) CLIA Kit

abx195057-96tests 96 tests
EUR 990

ELISA kit for Human Resistin

KET6029-48T 48T
EUR 242.4
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Resistin

KET6029-5platesof96wells 5 plates of 96 wells
EUR 1292.4
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Resistin

KET6029-96T 96T
EUR 346.8
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Resistin

GT15031 100 ug
EUR 662.4

Resistin

cyt-456 5µg
EUR 60
Description: Recombinant Human Resistin

Resistin

AP89468 1mg
EUR 2640

Resistin

AP78694 1mg
EUR 2640

Resistin

AP78695 1mg
EUR 2640

Resistin

RA19059 100 ul
EUR 451.2

Human Resistin (RETN) ELISA Kit

DLR-RETN-Hu 96T
EUR 380
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Prolonged-term wildlife mortality surveillance in northern Congo: a model for the detection of Ebola virus sickness epizootics.

Ebolavirus (EBOV) has introduced on sickness outbreaks taking 1000’s of lives, costing billions of {{dollars}} in administration efforts and threatening good ape populations. EBOV ecology is simply not completely understood nonetheless contaminated wildlife and consumption of animal carcasses have been linked to human outbreaks, significantly inside the Congo Basin.
Partnering with the Congolese Ministry of Nicely being, we carried out wildlife mortality surveillance and educational outreach inside the northern Republic of Congo (RoC). Designed for EBOV detection and to alert public effectively being authorities, we established a low-cost wildlife mortality reporting group overlaying 50 000 km2.
Concurrently, we delivered educational outreach promoting behavioural change to over 6600 people in rural northern RoC. We achieved specimen assortment by teaching endeavor staff on a safe sampling protocol and equipping geographically distributed bases with sampling kits.
We established in-country diagnostics for EBOV testing, decreasing diagnostic turnaround time to a few days and demonstrated the absence of EBOV in 58 carcasses. Central Africa stays a high-risk EBOV space, nonetheless RoC, dwelling to an important remaining populations of good apes, has not had an epidemic since 2005.
This effort continues to function as an untested early warning system in RoC, the place people and good apes have died from earlier Ebola virus sickness outbreaks. This article is part of the theme scenario ‘Dynamic and integrative approaches to understanding pathogen spillover’.