sebov-detection

A Survey of Virus Recombination Uncovers Canonical Choices of Artificial Chimeras Generated All through Deep Sequencing Library Preparation.

Chimeric reads shall be generated by in vitro recombination all through the preparation of high-throughput sequencing libraries. Our attempt to detect natural recombination between the genomes of dengue virus (DENV; +ssRNA genome) and its mosquito host using the Illumina Nextera sequencing library preparation bundle revealed that almost all, if not all, detected host-virus chimeras had been artificial.
Actually, these chimeras weren’t further frequent than with administration RNA from one different species (a pillbug), which was certainly not in contact with DENV RNA earlier to the library preparation.
The proportion of chimera varieties merely mirrored these of the three species amongst sequencing reads. Chimeras had been constantly characterised by the presence of 1-20 bp microhomology between recombining fragments. Inside-species chimeras largely involved fragments in reverse orientations and located decrease than 100 bp from each other inside the parental genome.
We found associated choices in revealed datasets using two totally different viruses: Ebola virus (EBOV; -ssRNA genome) and a herpesvirus (dsDNA genome), every produced with the Illumina Nextera protocol. These canonical choices advocate that artificial chimeras are generated by intra-molecular template switching of the DNA polymerase all through the PCR step of the Nextera protocol.
Lastly, a printed Illumina dataset using the Flock House virus (FHV; +ssRNA genome) generated with a protocol stopping artificial recombination revealed the presence of 1-10 bp microhomology motifs in FHV-FHV chimeras, nonetheless only some recombining fragments had been in reverse orientations.
Our analysis uncovered sequence choices characterizing recombination breakpoints in short-read sequencing datasets, which shall be helpful to guage the presence and extent of artificial recombination.
sebov-detection

sebov-detection

Human Resistin

MBS692069-0005mg 0.005mg
EUR 300

Human Resistin

MBS692069-0025mg 0.025mg
EUR 450

Human Resistin

MBS692069-5x0025mg 5x0.025mg
EUR 1725

Human resistin

MBS400069-001mg 0.01mg
EUR 220

Human resistin

MBS400069-005mg 0.05mg
EUR 545

Human resistin

MBS400069-01mg 0.1mg
EUR 770

Human resistin

MBS400069-1mg 1mg
EUR 3830

Human Resistin Antibody

32138-05111 150 ug
EUR 215

Human Resistin Antibody

GWB-F7AAE4 0.1 mg Ask for price

Resistin (43-65) (Human) - Antibody

H-028-39 50 μl
EUR 604.8

Resistin (51-108) (Human) - Antibody

H-028-40 50 μl
EUR 604.8

Resistin (43-65) (Human)

028-39 100 μg
EUR 255.96

Resistin (51-108) (Human)

028-40 20 μg
EUR 282.96

Resistin (23-42) (Human)

028-41 100 μg
EUR 214.92

Resistin (25-42) (Human)

028-42 100 μg
EUR 214.92

Mouse Anti-Human Resistin

MBS690775-05mg 0.5mg
EUR 525

Mouse Anti-Human Resistin

MBS690775-5x05mg 5x0.5mg
EUR 2080

Anti-Human Resistin Antibody

101-M170 500 µg
EUR 246.75
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5 kDa disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.

Anti-Human Resistin Antibody

101-M618 100 µg
EUR 399
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis.

Anti-Human Resistin Antibody

102-P92G 100 µg
EUR 245.7
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis.

Human Resistin (RETN)

1-CSB-EP019573HU
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Resistin(RETN) expressed in E.coli

Human resistin antagonist

MBS400115-001mg 0.01mg
EUR 220

Human resistin antagonist

MBS400115-005mg 0.05mg
EUR 545

Human resistin antagonist

MBS400115-01mg 0.1mg
EUR 770

Human resistin antagonist

MBS400115-1mg 1mg
EUR 3830

Biotinylated Anti-Human Resistin

GWB-BIG87D 1 kit Ask for price

Recombinant Human Resistin

7-01519 5µg Ask for price

Recombinant Human Resistin

7-01520 25µg Ask for price

Recombinant Human Resistin

7-01521 1mg Ask for price

RECOMBINANT HUMAN RESISTIN

GWB-F4A137 0.02 mg Ask for price

Recombinant Human Resistin

cyt-456-1mg 1mg
EUR 4680

Recombinant Human Resistin

cyt-456-25g 25µg
EUR 145

Recombinant Human Resistin

cyt-456-5g 5µg
EUR 60

Recombinant Human Resistin

MBS7611410-005mg 0.05mg
EUR 345

Recombinant Human Resistin

MBS7611410-02mg 0.2mg
EUR 635

Recombinant Human Resistin

MBS7611410-1mg 1mg
EUR 1800

Recombinant Human Resistin

MBS7611410-5x1mg 5x1mg
EUR 6955

Recombinant Human Resistin

MBS142332-0005mg 0.005mg
EUR 240

Recombinant Human Resistin

MBS142332-0025mg 0.025mg
EUR 310

Recombinant Human Resistin

MBS142332-1mg 1mg
EUR 4895

Recombinant Human Resistin

MBS142332-5x1mg 5x1mg
EUR 21695

Recombinant Human Resistin

MBS1410331-002mgEColi 0.02mg(E-Coli)
EUR 255

Recombinant Human Resistin

MBS1410331-01mgEColi 0.1mg(E-Coli)
EUR 405

Recombinant Human Resistin

MBS1410331-1mgEColi 1mg(E-Coli)
EUR 1425

Recombinant Human Resistin

MBS1410331-5x1mgEColi 5x1mg(E-Coli)
EUR 6215

RECOMBINANT HUMAN RESISTIN

MBS232425-0025mg 0.025mg
EUR 510

RECOMBINANT HUMAN RESISTIN

MBS232425-5x0025mg 5x0.025mg
EUR 2130

Human Resistin Recombinant

MBS553192-0005mg 0.005mg
EUR 165

Human Resistin Recombinant

MBS553192-0025mg 0.025mg
EUR 245

Recombinant Human Resistin

MBS9420612-002mg 0.02mg
EUR 285

Recombinant Human Resistin

MBS9420612-01mg 0.1mg
EUR 470

Recombinant Human Resistin

MBS9420612-1mg 1mg
EUR 1785

Recombinant Human Resistin

MBS9420612-5x1mg 5x1mg
EUR 7885

Human Resistin CLIA Kit

MBS8425884-INQUIRE INQUIRE Ask for price

Resistin, human recombinant

4559-100 each
EUR 874.8

Resistin, human recombinant

4559-1000 each
EUR 6127.2

Resistin, human recombinant

4559-25 each
EUR 307.2

Resistin (Recombinant) Human

GWB-8EE40A 0.025 mg Ask for price

Resistin, recombinant (Human)

028-36 20 μg
EUR 423.36

Resistin Human Recombinant

rAP-0727 Inquiry Ask for price

Human Resistin ELISA kit

E01R0351-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01R0351-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01R0351-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01A7519 96T
EUR 700
Description: ELISA

Human Resistin ELISA Kit

E16HE0035 96T
EUR 833.33

Human Resistin ELISA KIT

E16HE0035-048 48 wells
EUR 295

Human Resistin ELISA KIT

E16HE0035-096 96 wells
EUR 395

Human Resistin ELISA KIT

EKC40240-48T 48T
EUR 412.02

Human Resistin ELISA KIT

EKC40240-5x96T 5x96T
EUR 2795.85

Human Resistin ELISA KIT

EKC40240-96T 96T
EUR 588.6

Human Resistin ELISA KIT

E42EH-93 96T/48T Ask for price

Human Resistin ELISA kit

E22-HC136.48 48T
EUR 295

Human Resistin ELISA kit

E22-HC136.96 96T
EUR 395

Human Resistin ELISA Kit

E28L0045 96T
EUR 666.67

Human Resistin ELISA Kit

EK2351 96 tests
EUR 497

Human Resistin ELISA Kit

EHR0358 96Tests
EUR 625.2

Human Resistin ELISA kit

55R-E50 96 wells
EUR 715
Description: ELISA kit for the detection of Human Resistin in the research laboratory

Human Resistin ELISA kit

55R-1714 1 kit
EUR 717
Description: ELISA kit for the detection of Human Resistin in the research laboratory

Human Resistin ELISA kit

55R-2127 96 tests
EUR 825
Description: ELISA Kit for detection of Resistin in the research laboratory

Human Resistin ELISA KIT

CSB-E06884h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Resistin ELISA KIT

1-CSB-E06884h
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

human Resistin ELISA KIT

CN-04165H1 96T
EUR 530.4

human Resistin ELISA KIT

CN-04165H2 48T
EUR 351.6

Human Resistin ELISA Kit

MBS008924-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Resistin ELISA Kit

MBS008924-48StripWells 48-Strip-Wells
EUR 550

Human Resistin ELISA Kit

MBS008924-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Resistin ELISA Kit

MBS008924-96StripWells 96-Strip-Wells
EUR 765

Human Resistin ELISA Kit

GWB-SKR059 96 Tests Ask for price

Human Resistin ELISA Kit

GWB-ZZD207 1x96 well plate Ask for price

Human Resistin ELISA kit

LF-EK50441 1×96T
EUR 790.8

Human Resistin ELISA Kit

LF-EK70021 1×96T
EUR 625.2

Human Resistin ELISA Kit

RK00139 96 Tests
EUR 280

Human Resistin ELISA Kit

SL1530Hu 96 Tests
EUR 468

Human Resistin ELISA Kit

MBS720386-48StripWellsCompetitive 48-Strip-Wells-(Competitive)
EUR 485

Human Resistin ELISA Kit

MBS720386-48StripWellsSandwich 48-Strip-Wells-(Sandwich)
EUR 485

Human Resistin ELISA Kit

MBS720386-5x96StripWellsCompetitive 5x96-Strip-Wells-(Competitive)
EUR 3020

Prolonged-term wildlife mortality surveillance in northern Congo: a model for the detection of Ebola virus sickness epizootics.

Ebolavirus (EBOV) has introduced on sickness outbreaks taking 1000’s of lives, costing billions of {{dollars}} in administration efforts and threatening good ape populations. EBOV ecology is simply not completely understood nonetheless contaminated wildlife and consumption of animal carcasses have been linked to human outbreaks, significantly inside the Congo Basin.
Partnering with the Congolese Ministry of Nicely being, we carried out wildlife mortality surveillance and educational outreach inside the northern Republic of Congo (RoC). Designed for EBOV detection and to alert public effectively being authorities, we established a low-cost wildlife mortality reporting group overlaying 50 000 km2.
Concurrently, we delivered educational outreach promoting behavioural change to over 6600 people in rural northern RoC. We achieved specimen assortment by teaching endeavor staff on a safe sampling protocol and equipping geographically distributed bases with sampling kits.
We established in-country diagnostics for EBOV testing, decreasing diagnostic turnaround time to a few days and demonstrated the absence of EBOV in 58 carcasses. Central Africa stays a high-risk EBOV space, nonetheless RoC, dwelling to an important remaining populations of good apes, has not had an epidemic since 2005.
This effort continues to function as an untested early warning system in RoC, the place people and good apes have died from earlier Ebola virus sickness outbreaks. This article is part of the theme scenario ‘Dynamic and integrative approaches to understanding pathogen spillover’.