sebov-detection

A Survey of Virus Recombination Uncovers Canonical Choices of Artificial Chimeras Generated All through Deep Sequencing Library Preparation.

Chimeric reads shall be generated by in vitro recombination all through the preparation of high-throughput sequencing libraries. Our attempt to detect natural recombination between the genomes of dengue virus (DENV; +ssRNA genome) and its mosquito host using the Illumina Nextera sequencing library preparation bundle revealed that almost all, if not all, detected host-virus chimeras had been artificial.
Actually, these chimeras weren’t further frequent than with administration RNA from one different species (a pillbug), which was certainly not in contact with DENV RNA earlier to the library preparation.
The proportion of chimera varieties merely mirrored these of the three species amongst sequencing reads. Chimeras had been constantly characterised by the presence of 1-20 bp microhomology between recombining fragments. Inside-species chimeras largely involved fragments in reverse orientations and located decrease than 100 bp from each other inside the parental genome.
We found associated choices in revealed datasets using two totally different viruses: Ebola virus (EBOV; -ssRNA genome) and a herpesvirus (dsDNA genome), every produced with the Illumina Nextera protocol. These canonical choices advocate that artificial chimeras are generated by intra-molecular template switching of the DNA polymerase all through the PCR step of the Nextera protocol.
Lastly, a printed Illumina dataset using the Flock House virus (FHV; +ssRNA genome) generated with a protocol stopping artificial recombination revealed the presence of 1-10 bp microhomology motifs in FHV-FHV chimeras, nonetheless only some recombining fragments had been in reverse orientations.
Our analysis uncovered sequence choices characterizing recombination breakpoints in short-read sequencing datasets, which shall be helpful to guage the presence and extent of artificial recombination.
sebov-detection

sebov-detection

Anti-Resistin (1G10)
YF-MA18967 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (5E8)
YF-MA18968 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (4B11)
YF-MA18969 200 ul
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (2H8)
YF-MA18970 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (4C8)
YF-MA18971 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (6H8)
YF-MA18972 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (4H8)
YF-MA18973 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin (6C3)
YF-MA18974 100 ug
EUR 363
Description: Mouse monoclonal to Resistin
Anti-Resistin/RETN Antibody
PB9412 100ug/vial
EUR 294
Anti-Resistin/RETN Antibody
PA1473 100ug/vial
EUR 334
Resistin
GT15031 100 ug
EUR 552
Resistin
RA19059 100 ul
EUR 376
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280
Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280
Recombinant Human Resistin
7-01519 5µg Ask for price
Recombinant Human Resistin
7-01520 25µg Ask for price
Recombinant Human Resistin
7-01521 1mg Ask for price
Resistin, human recombinant
4559-100
EUR 729
Resistin, human recombinant
4559-1000
EUR 5106
Resistin, human recombinant
4559-25
EUR 256
Human Resistin Antibody
32138-05111 150 ug
EUR 261
Human Resistin (RETN)
1-CSB-EP019573HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 25.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Resistin(RETN) expressed in E.coli
Canine Resistin(Resistin) ELISA Kit
QY-E70171 96T
EUR 426
Rabbit Anti-Human Resistin/FIZZ3 protein antiserum #3
RSTN13-S 100 ul
EUR 457
rHu Resistin
AK9086-0005 5µg Ask for price
rHu Resistin
AK9086-0025 25µg Ask for price
rHu Resistin
AK9086-0100 100µg Ask for price
rHu Resistin
AK9086-1000 1mg Ask for price
Resistin Protein
20-abx262050
  • EUR 4615.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Resistin Protein
20-abx262253
  • EUR 5826.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Resistin Protein
20-abx262254
  • EUR 5826.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Resistin Protein
20-abx262255
  • EUR 5826.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.
Resistin antibody
70R-51393 100 ul
EUR 244
Description: Purified Polyclonal Resistin antibody
Resistin antibody
70R-RG001 100 ug
EUR 349
Description: Affinity purified Goat polyclonal Resistin antibody
Resistin antibody
70R-RG003 50 ug
EUR 273
Description: Affinity purified Goat polyclonal Resistin antibody
Resistin antibody
70R-RG005 50 ug
EUR 273
Description: Affinity purified Goat polyclonal Resistin antibody
Resistin antibody
70R-RR018 100 ug
EUR 300
Description: Affinity purified Rabbit polyclonal Resistin antibody
Resistin antibody
10R-R122A 500 ug
EUR 273
Description: Mouse monoclonal Resistin antibody
Resistin protein
30R-2640 25 ug
EUR 302
Description: Purified recombinant Human Resistin protein
Resistin protein
30R-2641 25 ug
EUR 317
Description: Purified recombinant Mouse Resistin protein
Resistin protein
30R-2642 25 ug
EUR 302
Description: Purified recombinant Rat Resistin protein
Resistin protein
30R-AR019 25 ug
EUR 273
Description: Purified recombinant Human Resistin protein
Resistin protein
30R-AR020 25 ug
EUR 273
Description: Purified recombinant Human Resistin protein
Resistin protein
30R-AR032 25 ug
EUR 273
Description: Purified recombinant Human Resistin protein
Resistin antibody
20R-1870 100 ug
EUR 673
Description: Rabbit polyclonal Resistin antibody
Resistin antibody
20R-1872 100 ug
EUR 673
Description: Rabbit polyclonal Resistin antibody
Resistin Antibody
5561-100
EUR 316
Resistin Antibody
5561-30T
EUR 146
Resistin Antibody
5997-100
EUR 316
Resistin Antibody
5997-30T
EUR 146
Resistin Antibody
5999-100
EUR 316
Resistin Antibody
5999-30T
EUR 146
Resistin antibody
70R-12369 100 ug
EUR 403
Description: Rabbit polyclonal Resistin antibody
Resistin antibody
70R-12461 100 ug
EUR 403
Description: Rabbit polyclonal Resistin antibody
Resistin antibody
70R-12463 100 ug
EUR 403
Description: Rabbit polyclonal Resistin antibody
Resistin antibody
70R-14012 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Resistin antibody
Human Resistin (RETN) Protein
abx670217-25ug 25 ug
EUR 551
  • Shipped within 1 week.
Human Resistin (RETN) Protein
20-abx651562
  • EUR 1024.00
  • EUR 356.00
  • EUR 3376.00
  • EUR 1233.00
  • EUR 704.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Human Resistin ELISA kit
E01R0351-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Resistin ELISA kit
E01R0351-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Resistin ELISA kit
E01R0351-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Resistin ELISA Kit
EHR0358 96Tests
EUR 521
Human Resistin (RETN) Protein
20-abx068871
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Recombinant Human Resistin Mutant
7-01525 2µg Ask for price
Recombinant Human Resistin Mutant
7-01526 10µg Ask for price
Recombinant Human Resistin Mutant
7-01527 1mg Ask for price
human Resistin ELISA KIT
201-12-0339 96 tests
EUR 440
  • This Resistin ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Resistin ELISA kit
55R-1714 1 kit
EUR 671
Description: ELISA kit for the detection of Human Resistin in the research laboratory
Human Resistin ELISA kit
55R-2127 96 tests
EUR 766
Description: ELISA Kit for detection of Resistin in the research laboratory
Human Resistin ELISA kit
55R-E50 96 wells
EUR 1178
Description: ELISA kit for the detection of Human Resistin in the research laboratory
Human Resistin ELISA KIT
CSB-E06884h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Resistin ELISA KIT
1-CSB-E06884h
  • EUR 602.00
  • EUR 4238.00
  • EUR 2256.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
human Resistin ELISA KIT
CN-04165H1 96T
EUR 442
human Resistin ELISA KIT
CN-04165H2 48T
EUR 293
Resistin, human recombinant protein
P1051-.025 25 µg
EUR 313
Description: Resistance-to-insulin (Resistin), also known as adipocyte-specific secretory factor (ADSF), is a 10 kDa secreted cysteine-rich peptide hormone. Resistin is a tissue-specific cytokine and inhibits insulin?s ability to stimulate glucose uptake.
Resistin, human recombinant protein
P1051-.1 100 µg
EUR 877
Description: Resistance-to-insulin (Resistin), also known as adipocyte-specific secretory factor (ADSF), is a 10 kDa secreted cysteine-rich peptide hormone. Resistin is a tissue-specific cytokine and inhibits insulin?s ability to stimulate glucose uptake.
Resistin, human recombinant protein
P1051-1 1 mg
EUR 6522
Description: Resistance-to-insulin (Resistin), also known as adipocyte-specific secretory factor (ADSF), is a 10 kDa secreted cysteine-rich peptide hormone. Resistin is a tissue-specific cytokine and inhibits insulin?s ability to stimulate glucose uptake.
Resistin Human Recombinant Protein
PROTQ9HD89-1 Regular: 25ug
EUR 317
Description: 9.9 kDa protein containing 93 amino acid residues.
Recombinant Human Resistin Protein
PROTQ9HD89-4 25ug
EUR 317
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5 kDa disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.
Human Resistin ELISA kit
LF-EK50441 1×96T
EUR 659
Human Resistin ELISA Kit
LF-EK70021 1×96T
EUR 521
Human Resistin ELISA Kit
RK00139 96 Tests
EUR 521
Rabbit Anti-Human Resistin/FIZZ3 IgG #2, aff pure
RSTN12-A 100 ug
EUR 482
Polyclonal Resistin Antibody
AMR09728G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Resistin . This antibody is tested and proven to work in the following applications:
Active Resistin (RETN)
4-APA847Hu61
  • EUR 689.57
  • EUR 287.00
  • EUR 2310.88
  • EUR 836.96
  • EUR 1573.92
  • EUR 524.00
  • EUR 5627.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9HD89
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: Inquire
Description: Recombinant Human Active Resistin (RETN) expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
Recombinant Mouse Resistin
CC127-005 5ug
EUR 293
Recombinant Mouse Resistin
CC127-025 25ug
EUR 408
Recombinant Mouse Resistin
CC127-101 1mg
EUR 10310
Eukaryotic Resistin (RETN)
4-EPA847Hu61
  • EUR 745.12
  • EUR 302.00
  • EUR 2519.20
  • EUR 906.40
  • EUR 1712.80
  • EUR 562.00
  • EUR 6148.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9HD89
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 36.8kDa
  • Isoelectric Point: 5.7
Description: Recombinant Human Resistin expressed in: 293F cell
Resistin Blocking Peptide
20-abx064268
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
Resistin (RETN) Antibody
20-abx129969
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Resistin (RETN) Antibody
20-abx131708
  • EUR 328.00
  • EUR 829.00
  • EUR 439.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-7 working days.
Resistin (RETN) Antibody
abx122576-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Resistin (RETN) Antibody
20-abx104852
  • EUR 411.00
  • EUR 133.00
  • EUR 1163.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Resistin (RETN) Antibody
20-abx100288
  • EUR 300.00
  • EUR 133.00
  • EUR 787.00
  • EUR 411.00
  • EUR 258.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Prolonged-term wildlife mortality surveillance in northern Congo: a model for the detection of Ebola virus sickness epizootics.

Ebolavirus (EBOV) has introduced on sickness outbreaks taking 1000’s of lives, costing billions of {{dollars}} in administration efforts and threatening good ape populations. EBOV ecology is simply not completely understood nonetheless contaminated wildlife and consumption of animal carcasses have been linked to human outbreaks, significantly inside the Congo Basin.
Partnering with the Congolese Ministry of Nicely being, we carried out wildlife mortality surveillance and educational outreach inside the northern Republic of Congo (RoC). Designed for EBOV detection and to alert public effectively being authorities, we established a low-cost wildlife mortality reporting group overlaying 50 000 km2.
Concurrently, we delivered educational outreach promoting behavioural change to over 6600 people in rural northern RoC. We achieved specimen assortment by teaching endeavor staff on a safe sampling protocol and equipping geographically distributed bases with sampling kits.
We established in-country diagnostics for EBOV testing, decreasing diagnostic turnaround time to a few days and demonstrated the absence of EBOV in 58 carcasses. Central Africa stays a high-risk EBOV space, nonetheless RoC, dwelling to an important remaining populations of good apes, has not had an epidemic since 2005.
This effort continues to function as an untested early warning system in RoC, the place people and good apes have died from earlier Ebola virus sickness outbreaks. This article is part of the theme scenario ‘Dynamic and integrative approaches to understanding pathogen spillover’.