sebov-detection

A Survey of Virus Recombination Uncovers Canonical Choices of Artificial Chimeras Generated All through Deep Sequencing Library Preparation.

Chimeric reads shall be generated by in vitro recombination all through the preparation of high-throughput sequencing libraries. Our attempt to detect natural recombination between the genomes of dengue virus (DENV; +ssRNA genome) and its mosquito host using the Illumina Nextera sequencing library preparation bundle revealed that almost all, if not all, detected host-virus chimeras had been artificial.
Actually, these chimeras weren’t further frequent than with administration RNA from one different species (a pillbug), which was certainly not in contact with DENV RNA earlier to the library preparation.
The proportion of chimera varieties merely mirrored these of the three species amongst sequencing reads. Chimeras had been constantly characterised by the presence of 1-20 bp microhomology between recombining fragments. Inside-species chimeras largely involved fragments in reverse orientations and located decrease than 100 bp from each other inside the parental genome.
We found associated choices in revealed datasets using two totally different viruses: Ebola virus (EBOV; -ssRNA genome) and a herpesvirus (dsDNA genome), every produced with the Illumina Nextera protocol. These canonical choices advocate that artificial chimeras are generated by intra-molecular template switching of the DNA polymerase all through the PCR step of the Nextera protocol.
Lastly, a printed Illumina dataset using the Flock House virus (FHV; +ssRNA genome) generated with a protocol stopping artificial recombination revealed the presence of 1-10 bp microhomology motifs in FHV-FHV chimeras, nonetheless only some recombining fragments had been in reverse orientations.
Our analysis uncovered sequence choices characterizing recombination breakpoints in short-read sequencing datasets, which shall be helpful to guage the presence and extent of artificial recombination.
sebov-detection

sebov-detection

Human Resistin ELISA kit

55R-2127 96 tests
EUR 919.2
Description: ELISA Kit for detection of Resistin in the research laboratory

Human Resistin ELISA kit

55R-E50 96 wells
EUR 1413.6
Description: ELISA kit for the detection of Human Resistin in the research laboratory

human Resistin ELISA KIT

CN-04165H1 96T
EUR 530.4

human Resistin ELISA KIT

CN-04165H2 48T
EUR 351.6

Human Resistin ELISA KIT

CSB-E06884h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Resistin ELISA KIT

1-CSB-E06884h
  • EUR 722.40
  • EUR 5085.60
  • EUR 2707.20
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Resistin ELISA kit

E01R0351-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01R0351-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

E01R0351-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Resistin ELISA kit

LF-EK50441 1×96T
EUR 790.8

Human Resistin ELISA Kit

LF-EK70021 1×96T
EUR 625.2

Human Resistin ELISA Kit

EHR0358 96Tests
EUR 625.2

Human Resistin ELISA Kit

RK00139 96 Tests
EUR 625.2

Resistin, human recombinant

4559-100 each
EUR 874.8

Resistin, human recombinant

4559-1000 each
EUR 6127.2

Resistin, human recombinant

4559-25 each
EUR 307.2

Resistin

GT15031 100 ug
EUR 662.4

Resistin

RA19059 100 ul
EUR 451.2

Human Resistin (RETN) Protein

20-abx068871
  • EUR 777.60
  • EUR 326.40
  • EUR 2331.60
  • EUR 910.80
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Resistin (RETN) Protein

20-abx651562
  • EUR 1228.80
  • EUR 427.20
  • EUR 4051.20
  • EUR 1479.60
  • EUR 844.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Resistin (RETN) Protein

abx670217-25ug 25 ug
EUR 661.2

Resistin antibody

10R-R122A 500 ug
EUR 327.6
Description: Mouse monoclonal Resistin antibody

Resistin antibody

20R-1870 100 ug
EUR 807.6
Description: Rabbit polyclonal Resistin antibody

Resistin antibody

20R-1872 100 ug
EUR 807.6
Description: Rabbit polyclonal Resistin antibody

Resistin antibody

70R-RG001 100 ug
EUR 418.8
Description: Affinity purified Goat polyclonal Resistin antibody

Resistin antibody

70R-RG003 50 ug
EUR 327.6
Description: Affinity purified Goat polyclonal Resistin antibody

Resistin antibody

70R-RG005 50 ug
EUR 327.6
Description: Affinity purified Goat polyclonal Resistin antibody

Resistin antibody

70R-RR018 100 ug
EUR 360
Description: Affinity purified Rabbit polyclonal Resistin antibody

Resistin Antibody

5997-100 each
EUR 379.2

Resistin Antibody

5997-30T each
EUR 175.2

Resistin Antibody

5999-100 each
EUR 379.2

Resistin Antibody

5999-30T each
EUR 175.2

Resistin Antibody

5561-100 each
EUR 379.2

Resistin Antibody

5561-30T each
EUR 175.2

Resistin antibody

70R-12369 100 ug
EUR 483.6
Description: Rabbit polyclonal Resistin antibody

Resistin antibody

70R-12461 100 ug
EUR 483.6
Description: Rabbit polyclonal Resistin antibody

Resistin antibody

70R-12463 100 ug
EUR 483.6
Description: Rabbit polyclonal Resistin antibody

Resistin antibody

70R-14012 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal Resistin antibody

Resistin antibody

70R-51393 100 ul
EUR 292.8
Description: Purified Polyclonal Resistin antibody

Resistin Antibody

R30545 100 ug
EUR 356.15
Description: Resistin, also known as adipose tissue-specific secretory factor(ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein(XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus.

Resistin Antibody

R32326 100 ug
EUR 356.15
Description: Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus.

Human Resistin (RETN) CLIA Kit

abx195057-96tests 96 tests
EUR 990

Human Resistin (RETN) CLIA Kit

20-abx492128
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

ELISA kit for Human Resistin

KET6029-48T 48T
EUR 242.4
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Resistin

KET6029-5platesof96wells 5 plates of 96 wells
EUR 1292.4
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Resistin

KET6029-96T 96T
EUR 346.8
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Resistin

EK2351 96 tests
EUR 542.4
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Resistin in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Resistin (RETN) ELISA Kit

abx252068-96tests 96 tests
EUR 764.4

Human Resistin (RETN) ELISA Kit

abx050199-96tests 96 tests
EUR 801.6

Human Resistin (RETN) ELISA Kit

abx051478-96tests 96 tests
EUR 801.6

Human Resistin (RETN) ELISA Kit

abx575492-96tests 96 tests
EUR 764.4

Human Resistin, RETN ELISA Kit

DEIA139 15 Plates
EUR 2889.6
Description: Human RETN ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant RETN in a sandwich ELISA format within the range of 16-2,000 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay RETN in approximately 1,500 ELISA plate wells.

Human Resistin, RETN ELISA Kit

DEIA221 96T
EUR 1020
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human RETN in serum, plasma and other biological fluids.

Human Resistin (RETN) ELISA Kit

DLR-RETN-Hu-48T 48T
EUR 529.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Resistin (RETN) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Resistin (RETN) ELISA Kit

DLR-RETN-Hu-96T 96T
EUR 684
Description: A sandwich quantitative ELISA assay kit for detection of Human Resistin (RETN) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Resistin (RETN) ELISA Kit

20-abx152952
  • EUR 6768.00
  • EUR 3610.80
  • EUR 848.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Resistin(RETN)ELISA Kit

GA-E0355HM-48T 48T
EUR 346.8

Human Resistin(RETN)ELISA Kit

GA-E0355HM-96T 96T
EUR 559.2

Human Resistin, RETN ELISA KIT

ELI-02884h 96 Tests
EUR 988.8

Human Resistin (RETN) ELISA Kit

RD-RETN-Hu-48Tests 48 Tests
EUR 523.2

Human Resistin (RETN) ELISA Kit

RD-RETN-Hu-96Tests 96 Tests
EUR 721.2

Human Resistin (RETN) ELISA Kit

RDR-RETN-Hu-48Tests 48 Tests
EUR 546

Human Resistin (RETN) ELISA Kit

RDR-RETN-Hu-96Tests 96 Tests
EUR 754.8

Human Resistin (RETN) ELISA Kit

QY-E05704 96T
EUR 433.2

Human Resistin(RETN)ELISA Kit

QY-E03808 96T
EUR 472.8

Human Resistin (RETN) ELISA Kit

SEA847Hu-10x96wellstestplate 10x96-wells test plate
EUR 4091.29
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Resistin (RETN) ELISA Kit

SEA847Hu-1x48wellstestplate 1x48-wells test plate
EUR 442.1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Resistin (RETN) ELISA Kit

SEA847Hu-1x96wellstestplate 1x96-wells test plate
EUR 580.15
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Resistin (RETN) ELISA Kit

SEA847Hu-5x96wellstestplate 5x96-wells test plate
EUR 2250.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Resistin (RETN) ELISA Kit

4-SEA847Hu
  • EUR 4152.00
  • EUR 2191.20
  • EUR 580.80
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Resistin (RETN) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

human Resistin(Retn) ELISA KIT

YLA0514HU-48T 48T
EUR 435

human Resistin(Retn) ELISA KIT

YLA0514HU-96T 96T
EUR 562.5

Human Resistin (RETN) ELISA Kit

RDR-RETN-Hu-48T 48T
EUR 389.55
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Resistin (RETN) ELISA Kit

RDR-RETN-Hu-96T 96T
EUR 556.5
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Resistin (RETN) ELISA Kit

RD-RETN-Hu-48T 48T
EUR 371
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Resistin (RETN) ELISA Kit

RD-RETN-Hu-96T 96T
EUR 530
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Resistin Antibody (Biotin Conjugate)

32138-05121 150 ug
EUR 442.8

Human Resistin ELISA kit (4X96T)

LF-EK50442 4×96T
EUR 2690.4

Recombinant Human Resistin Mutant

7-01525 2µg Ask for price

Recombinant Human Resistin Mutant

7-01526 10µg Ask for price

Recombinant Human Resistin Mutant

7-01527 1mg Ask for price

Human RETN/ Resistin ELISA Kit

E2142Hu 1 Kit
EUR 644.4

Human RETN(Resistin) ELISA Kit

EH0269 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Resistin (RETN) Monoclonal Antibody (Human)

4-MAA847Hu22
  • EUR 271.20
  • EUR 2616.00
  • EUR 660.00
  • EUR 336.00
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Resistin (RETN)

Resistin (RETN) Polyclonal Antibody (Human)

4-PAA847Hu01
  • EUR 260.40
  • EUR 2457.60
  • EUR 624.00
  • EUR 321.60
  • EUR 241.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Resistin (RETN)

Resistin (RETN) Polyclonal Antibody (Human)

4-PAA847Hu06
  • EUR 260.40
  • EUR 2457.60
  • EUR 624.00
  • EUR 321.60
  • EUR 241.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Resistin (RETN)

Recombinant Human Resistin Protein

PROTQ9HD89-4 25ug
EUR 380.4
Description: Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family; Resistin, RELM-α and RELM-β share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake, and postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5 kDa disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.

Resistin (human) Serum ELISA Kit

K4919-100 each
EUR 1149.6

rHu Resistin

AK9086-0005 5µg Ask for price

rHu Resistin

AK9086-0025 25µg Ask for price

rHu Resistin

AK9086-0100 100µg Ask for price

rHu Resistin

AK9086-1000 1mg Ask for price

Human Resistin-like beta (RETNLB)

1-CSB-YP860653HU
  • EUR 516.00
  • EUR 280.80
  • EUR 1809.60
  • EUR 770.40
  • EUR 1210.80
  • EUR 349.20
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Resistin-like beta(RETNLB) expressed in Yeast

Human Resistin-like beta (RETNLB)

1-CSB-EP860653HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Resistin-like beta(RETNLB) expressed in E.coli

Human Resistin AssayMax ELISA Kit

ER1001-1 96 Well Plate
EUR 572.4

Human Resistin PicoKine ELISA Kit

EK0581 96 wells
EUR 583.2
Description: For quantitative detection of human Resistin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Prolonged-term wildlife mortality surveillance in northern Congo: a model for the detection of Ebola virus sickness epizootics.

Ebolavirus (EBOV) has introduced on sickness outbreaks taking 1000’s of lives, costing billions of {{dollars}} in administration efforts and threatening good ape populations. EBOV ecology is simply not completely understood nonetheless contaminated wildlife and consumption of animal carcasses have been linked to human outbreaks, significantly inside the Congo Basin.
Partnering with the Congolese Ministry of Nicely being, we carried out wildlife mortality surveillance and educational outreach inside the northern Republic of Congo (RoC). Designed for EBOV detection and to alert public effectively being authorities, we established a low-cost wildlife mortality reporting group overlaying 50 000 km2.
Concurrently, we delivered educational outreach promoting behavioural change to over 6600 people in rural northern RoC. We achieved specimen assortment by teaching endeavor staff on a safe sampling protocol and equipping geographically distributed bases with sampling kits.
We established in-country diagnostics for EBOV testing, decreasing diagnostic turnaround time to a few days and demonstrated the absence of EBOV in 58 carcasses. Central Africa stays a high-risk EBOV space, nonetheless RoC, dwelling to an important remaining populations of good apes, has not had an epidemic since 2005.
This effort continues to function as an untested early warning system in RoC, the place people and good apes have died from earlier Ebola virus sickness outbreaks. This article is part of the theme scenario ‘Dynamic and integrative approaches to understanding pathogen spillover’.