Soluble Collagen Assay Kit |
MET-5016 |
Cell Biolabs |
96 assays |
EUR 644.4 |
Description: Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve. |
Collagen Assay Laboratories manufactures the sircol-based assay soluble collagen reagents distributed by Genprice. The Sircol-Based Assay Soluble Collagen reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact collagen assay. Other Sircol-Based products are available in stock. Specificity: Sircol-Based Category: Assay Group: Soluble Collagen
Total Collagen Assay Kit (Colorimetric) |
Biovision |
each |
EUR 744 |
Total Collagen Microplate Assay Kit |
DL Develop |
100 Assays |
EUR 420 |
Description: Detection and Quantification of Total Collagen Content. |
PDE4B Cell-Based Activity Assay Kit |
BPS Bioscience |
500 rxns. |
EUR 610 |
Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4B has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP. Inhibition of PDE4B activity by its inhibitors leads to an elevated intracellular level of cAMP. The PDE4B gene encodes at least 4 different isoforms, and has been linked to inflammation in monocytes by its regulation of the toll-receptor pathway. It is also highly expressed in the central nervous system, and has been targeted as a potential treatment for autism, depression, schizophrenia, and other conditions._x000D_The PDE4B cell-based activity assay kit is designed for screening inhibitors of PDE4B1 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4B1 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4B inhibitor to inhibit PDE4B1 activity, cAMP level is restored, resulting in higher luciferase activity._x000D_The kit includes CRE luciferase reporter (premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency), PDE4B1 expression vector, and forskolin. |
Human IDO1 Cell-Based Assay Kit |
BPS Bioscience |
100 rxns. |
EUR 595 |
Description: The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells. |
Human TDO Cell-Based Assay Kit |
BPS Bioscience |
100 rxns. |
EUR 565 |
Description: The human TDO Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human TDO in cultured cells. |
PDE4D Cell-Based Activity Assay Kit |
BPS Bioscience |
500 rxns. |
EUR 645 |
Description: The PDE4D cell-based activity assay is designed for screening inhibitors of PDE4D7 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. _x000D_CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. |
LDL Uptake Assay Kit (Cell-Based) |
Biovision |
each |
EUR 816 |
Soluble Collagen information
Total Collagen Assay Kit (Colorimetric) |
K218-100 |
Biovision |
each |
EUR 744 |
total collagen assay kit 2x 96-assays |
QZBtotcol2 |
Quickzyme |
2 plates 192 assays |
EUR 736.8 |
sensitive tissue collagen assay kit 96-assays |
QZBtiscol1 |
Quickzyme |
1 plate 96 assays |
EUR 493.2 |
Sirius Red Total Collagen Detection Assay PLATE Kit |
9062P |
Chondrex |
80 samples |
EUR 319.26 |
Description: Sirius Red Total Collagen Detection Assay PLATE Kit |
Total Collagen Assay Kit (Perchlorate-Free) |
K406-100 |
Biovision |
each |
EUR 744 |
Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric) |
K490-100 |
Biovision |
each |
EUR 516 |
sensitive tissue collagen assay kit 2x 96-assays |
QZBtiscol2 |
Quickzyme |
2 plates 192 assays |
EUR 910.8 |
Rapid Collagenase Assay Kit with Type II Collagen Substrate |
3002 |
Chondrex |
1 kit x2 plates |
EUR 872.58 |
Description: Rapid Collagenase Assay Kit with Type II Collagen Substrate |
total collagen assay kit 5x 96-assays bulk pack |
QZBtotcol5 |
Quickzyme |
5 plates 480 assays |
EUR 1435.2 |
Soluble Sugar Microplate Assay Kit |
DLSM0171 |
DL Develop |
100 Assays |
EUR 105 |
Description: Detection and Quantification of Soluble Sugar Content. |