Sircol-Based Assay Soluble Collagen

Soluble Collagen Assay Kit

MET-5016 96 assays
EUR 644.4
Description: Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve.

Collagen Assay Laboratories manufactures the sircol-based assay soluble collagen reagents distributed by Genprice. The Sircol-Based Assay Soluble Collagen reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact collagen assay. Other Sircol-Based products are available in stock. Specificity: Sircol-Based Category: Assay Group: Soluble Collagen

PDE4D Cell-Based Activity Assay Kit

500 rxns.
EUR 645
Description: The PDE4D cell-based activity assay is designed for screening inhibitors of PDE4D7 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. _x000D_CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity.

Human IDO1 Cell-Based Assay Kit

100 rxns.
EUR 595
Description: The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.

Human TDO Cell-Based Assay Kit

100 rxns.
EUR 565
Description: The human TDO Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human TDO in cultured cells.

PDE4B Cell-Based Activity Assay Kit

500 rxns.
EUR 610
Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4B has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP. Inhibition of PDE4B activity by its inhibitors leads to an elevated intracellular level of cAMP. The PDE4B gene encodes at least 4 different isoforms, and has been linked to inflammation in monocytes by its regulation of the toll-receptor pathway. It is also highly expressed in the central nervous system, and has been targeted as a potential treatment for autism, depression, schizophrenia, and other conditions._x000D_The PDE4B cell-based activity assay kit is designed for screening inhibitors of PDE4B1 in cultured cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4B1 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4B inhibitor to inhibit PDE4B1 activity, cAMP level is restored, resulting in higher luciferase activity._x000D_The kit includes CRE luciferase reporter (premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency), PDE4B1 expression vector, and forskolin.

LDL Uptake Assay Kit (Cell-Based)

each
EUR 816

Total Collagen Assay Kit (Perchlorate-Free)

each
EUR 744

total collagen assay kit 96-assays

1 plate 96 assays
EUR 400.8

Soluble Collagen information

EZCellTM Cell Invasion Assay (Collagen IV), 12-well, 8 µm

K919-12 each
EUR 614.4

CytoSelect 24-well Collagen Cell Invasion Assay, Fluorometric

CBA-111-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

Rat Anti-Rat Type I Collagen IgG Antibody Assay Kit, TMB

1024T 1 kit
EUR 709.02
Description: Rat Anti-Rat Type I Collagen IgG Antibody Assay Kit

Rat Anti-Rat Type II Collagen IgG Antibody Assay Kit, TMB

2044T 1 kit
EUR 790.8
Description: Rat Anti-Rat Type II Collagen IgG Antibody Assay Kit

Rat Anti-Rat Type II Collagen IgG Antibody Assay Kit, (OPD)

2044 1 kit
EUR 790.8
Description: Rat Anti-Rat Type II Collagen IgG Antibody Assay Kit

Rat Anti-Rat Type II Collagen IgG1 Antibody Assay Kit, TMB

20441T 1 kit
EUR 820.38
Description: Rat Anti-Rat Type II Collagen IgG1 Antibody Assay Kit

Rat Anti-Chick Type I Collagen IgG Antibody Assay Kit, TMB

1021T 1 kit
EUR 709.02
Description: Rat Anti-Chick Type I Collagen IgG Antibody Assay Kit

Rat Anti-Human Type I Collagen IgG Antibody Assay Kit, TMB

1025T 1 kit
EUR 773.4
Description: Rat Anti-Human Type I Collagen IgG Antibody Assay Kit

Rat Anti-Human Type II Collagen IgG Antibody Assay Kit, (OPD)

2045 1 kit
EUR 790.8
Description: Rat Anti-Human Type II Collagen IgG Antibody Assay Kit

Rat Anti-Human Type II Collagen IgG Antibody Assay Kit, TMB

2045T 1 kit
EUR 790.8
Description: Rat Anti-Human Type II Collagen IgG Antibody Assay Kit

Rat Anti-Chick Type II Collagen IgG Antibody Assay Kit, (OPD)

2041 1 kit
EUR 726.42
Description: Rat Anti-Chick Type II Collagen IgG Antibody Assay Kit

Rat Anti-Chick Type II Collagen IgG Antibody Assay Kit, TMB

2041T 1 kit
EUR 726.42
Description: Rat Anti-Chick Type II Collagen IgG Antibody Assay Kit

Rat Anti-Rat Type II Collagen IgG2a Antibody Assay Kit, TMB

20442T 1 kit
EUR 820.38
Description: Rat Anti-Rat Type II Collagen IgG2a Antibody Assay Kit

EZCellTM Cell Invasion Assay Kit (Collagen I), 12-well, 8 µm

K917-12 each
EUR 627.6

Collagenase assay, 10-30 samples

80042 Custom service
EUR 150.48
Description: Collagenase assay

Rat Anti-Bovine Type I Collagen IgG Antibody Assay Kit, TMB

1022T 1 kit
EUR 709.02
Description: Rat Anti-Bovine Type I Collagen IgG Antibody Assay Kit