EXODNA EXTRACTION KITS
Exosomes are small endosome-derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome. The release occurs constitutively or by induction, both under normal and pathological conditions, in a dynamic, regulated, and functional way. relevant way. Both the quantity and the molecular composition of the exosomes released depend on the state of the stem cell. Exosomes have been isolated from various cell lines (hematopoietic cells, tumor lines, primary cultures, and virus-infected cells) as well as from biological fluids in particular blood (for example, serum and plasma of cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva, etc.).
Exosomes have pleiotropic physiological and pathological functions and an emerging role in various pathological conditions such as cancer, infectious and neurodegenerative diseases. The ExoPureTM DNA Extraction Kit is for the extraction of exosomal DNA from purified exosomes isolated using Exosome Isolation Kits (cat. K1237-K1241). Typically, 100 ~ 300 ng of exosomal DNA can be purified from exosomes isolated from 200 μL of blood serum or 5 ml of cells media. The extracted DNA can be used directly for PCR and sequencing. It is not necessary to precipitate, concentrate or desalinate. II. Request:
• Easy to use
• The extracted DNA can be used directly for PCR and sequencing
• No need to precipitate, concentrate or desalt the extracted DNA
III. Example type:
• Cell media, serum, plasma, urine, biofluids