SET7/9 promotes H3K4me3 at lncRNA DRAIC promoter to modulate growth and metastasis of glioma
Goal: We geared toward investigating the expression ranges of SET7/9 in glioma and the connection between SET7/9 and LncRNA DRAIC. Additional, we explored the connection between SET7/9 and glioma cell metastasis and temper.
Sufferers and strategies: The expression ranges of DRAIC and miR-18a-3p in gastric most cancers cells have been measured by quantitative polymerase chain response (qPCR). The binding website of the promoter of DRAIC by H3K4me3 was confirmed by ChIP-Actual-time PCR. The direct goal of DRAIC and miR-18a-3p in gastric most cancers cells was measured by a Luciferase reporter assay. Cell proliferation was detected by Cell counting package-8 (CCK8), and cell invasion and migration have been measured by transwell assays.
Outcomes: In contrast with adjoining non-cancerous regular tissues, SET7/9 and DRAIC have been each downregulated and miR-18a-3p was upregulated in glioma cells. In the meantime, silencing of SET7/9 enhanced cell proliferation, migration, and invasion in U251 cells. H3K4me3 protein can bind to DRAIC promoter immediately. Inhibition of SET7/9 and downregulation of DRAIC in U251 cells reversed the impact of SET7/9 silencing on the expansion and metastasis of glioma cells. In U251 cells, SET7/9 and DRAIC overexpression inhibited cell proliferation, migration and invasion. As well as, miR-18a-3p interacts with DRAIC by means of direct binding. The inhibition of DRAIC promoted the expansion and metastasis of U251 cells, whereas the co-transfection of si- DRAIC and miR-18a-3p additional promoted the expansion and metastasis of U251 cells. Overexpression of DRAIC inhibited the expansion and metastasis of cells, utterly reversing the co-transfection of Lnc-DRAIC and miR-18a-3p.
Conclusions: On this analysis, we found that the expression of SET7/9 was low in glioma cells and SET7/9-mediated H3K4me3 enrichment on the DRAIC promoter regulated the expansion and metastasis of glioma cells by concentrating on miR-18a-3p. It doubtlessly supplies a brand new therapeutic marker concentrating on glioma.

miR-335-5P contributes to human osteoarthritis by concentrating on HBP1

MicroRNA (miR)-335-5P has the power to manage chondrogenic differentiation and promote chondrogenesis in mouse mesenchymal stem cells. Additionally it is abnormally elevated in human osteoarthritic chondrocytes. Nevertheless, the organic perform of miR-335-5P in osteoarthritis (OA) isn’t effectively understood. The current examine investigated the mechanism of miR-335-5P within the pathogenesis of OA. To analyze the impact of miR-335-5P on the pathogenesis of OA in vitro, a miR-335-5P mimic and inhibitor have been transfected into chondrocytes.
Cell Counting package-Eight assay and circulate cytometry have been used to observe the results of miR-335-5P on chondrocyte apoptosis and the expression of cartilage-specific genes, equivalent to aggrecan, collagen II, matrix metalloproteinase 13 and collagen X, have been detected by reverse transcription-quantitative PCR and western blot evaluation. Furthermore, the present examine assessed whether or not HMG-box transcription issue 1 (HBP1) is a novel goal of miR-335-5P with twin luciferase reporter assays. Lastly, a rescue experiment was used to show the regulation between miR-335-5P and HBP1.
The outcomes revealed that HBP1 was a novel goal of miR-335-5P, and that miR-335-5P mediated the apoptosis of chondrocytes and modifications in cartilage-specific genes by way of concentrating on HBP1. Total, the current examine revealed that miR-335-5P mediated the event of OA by concentrating on the HBP1 gene and selling chondrocyte apoptosis. These knowledge advised that miR-335-5P could also be used to develop novel early-stage diagnostic and therapeutic methods for OA.
 SET7/9 promotes H3K4me3 at lncRNA DRAIC promoter to modulate growth and metastasis of glioma

SET7/9 promotes H3K4me3 at lncRNA DRAIC promoter to modulate growth and metastasis of glioma

Lengthy non‑coding RNA XIST promotes cell proliferation of pancreatic most cancers by means of miR‑137 and Notch1 pathway

Goal: Lengthy non-coding ribonucleic acids X-inactive particular transcript (lncRNA XIST) is one lncRNAs which concerned in a number of human cancers. Nevertheless, the capabilities and potential molecular regulatory mechanisms of XIST/microRNA-137 (miR‑137) in pancreatic most cancers (PC) nonetheless have to discover.
Sufferers and strategies: PC tissues and cell strains have been analyzed for XIST, miR-137 and Notch1 expressions by means of quantitative real-time polymerase chain response (qRT-PCR) and Western blot. Nude mouse xenograft tumor assay was used to detect XIST results on pancreatic tumorigenesis in vivo. Cell Counting Equipment (CCK-8) assay was carried out to detect PC cell proliferation. Twin-Luciferase reporter assay, qRT-PCR, RNA immunoprecipitation (RIP) and Western blot assays have been utilized to validate the goal relationship of XIST, miR‑137 and Notch1.
Outcomes: Outcomes demonstrated that XIST expression was elevated in PC tissues and cells. XIST knockdown inhibited PC cell proliferation in vitro and likewise repressed the tumor progress in vivo. XIST immediately interacted with miR-137 and negatively regulated its expression. Notch1 was recognized as a goal gene of miR-137 and XIST acted as a aggressive endogenous RNA (ceRNA) to positively regulate Notch1 expression by suppressing miR-137. As well as, we detected miR-137 was negatively correlated with XIST and Notch1 respectively, and a constructive correlation between Notch1 and XIST expression in PC tissues. Moreover, Notch1 overexpression might offset the suppressing impact of XIST knockdown or miR-137 overexpression on cell proliferation. Due to this fact, XIST might play an vital function in selling cell proliferation by means of miR‑137 and Notch1 pathway in PC.

IgE Dilution Buffer

3009 1 mg/ml x 10 ml
EUR 96.54
Description: IgE Dilution Buffer

Library Dilution Buffer

abx098898-5ml 5 ml
EUR 309.6

Library Dilution Buffer

NQ106 50 ml
EUR 188.4

Topoisomerase II Dilution Buffer

TG4090 1ml
EUR 187.2

Topoisomerase I Dilution Buffer

TG4095 1ml
EUR 187.2

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (TBS)

Dilution buffer R (10x); ELISPOT

CT348 10 ml
EUR 93.6

Dilution buffer T (10x); ELISPOT

CT358 8 ml
EUR 93.6

Antibody Dilution Buffer for ICC and IHC

SF40010 10 ml
EUR 146.4

Dilution Buffer [1x PBS pH 7.3]

0209B 100 ml
EUR 92.4

Dilution buffer B (10x); ELISPOT (PVDF)

CT364 8 ml
EUR 93.6

ChonBlock Western Blot Buffer 2 for Goat (PBS)

90691P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)

90691T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

90692P 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

90692T 10 ml
EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

West-Ezier Antibody Dilution Buffer, ready-to-use

W3872-050 500ml
EUR 198

TnI (Detection) Antibody

abx018057-100ug 100 ug
EUR 410.4

CK19 (Detection) Antibody

abx019246-1mg 1 mg
EUR 794.4

PCT (Detection) Antibody

20-abx019248
  • EUR 469.20
  • EUR 744.00
  • 0.5 mg
  • 1 mg

Antibody diluent buffer

AR1106-2 12ml
EUR 109.2

HCl Dilution Matrix (10g/l HCl)

HADM1 1L
EUR 107.16

HCl Dilution Matrix 10g/L HCl

HADM1A 2ML
EUR 98.04

Kinesis Blood Dilution Vial; 25ml Bulk

CHR1062 PK1000
EUR 159.13

Dilution Membrane Protein Folding Screen Kit

MPS01-20 kit
EUR 117.02
Description: This product includes 0.1 ml of Reagent A, 0.1 ml of Reagent B, 1.1 ml of Reagent C, 0.35 ml of 20 Solutions 1-20.

Anti-ST2 Detection Antibody

A2099-100 100 µg
EUR 435.6

PCT (Detection) Antibody (Biotin)

abx019264-1mg 1 mg
EUR 1078.8

Albumin (Capture / Detection) Antibody

abx019293-1mg 1 mg
EUR 661.2

Albumin (Capture / Detection) Antibody

abx019294-1mg 1 mg
EUR 661.2

Fetal Fibronectin (Detection) Antibody

abx019300-1mg 1 mg
EUR 744

Fetal Fibronectin (Detection) Antibody

abx019302-1mg 1 mg
EUR 744

SCCA1 (Capture / Detection) Antibody

abx019308-1mg 1 mg
EUR 794.4

SCCA1 (Capture / Detection) Antibody

abx019309-1mg 1 mg
EUR 794.4

WB / Antibody Stripping Buffer

20-abx090656
  • EUR 226.80
  • EUR 276.00
  • 100 ml
  • 200 ml

WB / Antibody Stripping Buffer

abx090671-200ml 200 ml
EUR 226.8

Wash Buffer

1210-200
EUR 235.2

RIPA Buffer

2114-100
EUR 189.6

RIPA Buffer

2114-500
EUR 555.6

Tricine buffer

20-abx082551
  • EUR 260.40
  • EUR 226.80
  • 100 g
  • 25 g

Wash Buffer

abx098952-20ml 20 ml
EUR 109.2

Coating Buffer

abx098970-1vial 1 vial
EUR 184.8

Blocking Buffer

abx098972-1vial 1 vial
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer120ml Lysis Buffer 1 (20 ml)
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer3100ml Lysis Buffer 3 (100ml)
EUR 276

Lysis Buffer

abx098984-LysisBuffer420ml Lysis Buffer 4 (20 ml)
EUR 184.8

Binding Buffer

abx290019-50ml 50 ml
EUR 276

Wash Buffer

abx293002-30ml 30 ml
EUR 126

Binding Buffer

BB10X-50ML 50 ML
EUR 136.44

ADA buffer

AD0003 25g
EUR 77.75

Wash Buffer

KF17356 500 ml
EUR 214.8

RIPA Buffer

GR103019 50 mL
EUR 70.8

Stripping Buffer

GR103020 500 mL
EUR 106.8

Anode Buffer

GR103036 1 L
EUR 106.8

Cathode Buffer

GR103037 1 L
EUR 130.8

10xTaq Buffer

PCRB60 4x1.5ml, 6ml
EUR 70.44

PBS Buffer

RM00012 2L
EUR 122.4

Phosphate Buffer

PB1 1L
EUR 94.62

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers

5730 EACH
EUR 864.12

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-050 500ml
EUR 96

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-100 2X500ml
EUR 124.8

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-101 1L
EUR 114

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-200 4X500ml
EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-201 2X1L
EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-401 4X1L
EUR 198

TT Buffer (Tris-Tricine buffer) Primix powder

TD8133 1PK, 10L
EUR 91.32

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN

99-783-CM 1 L /pk
EUR 75.6
Description: Specialty Media; Islet products

Dilution Preparative Membrane Protein Folding Kit (Condition #1)

MPP01 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S1.

Dilution Preparative Membrane Protein Folding Kit (Condition #2)

MPP02 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S2

Dilution Preparative Membrane Protein Folding Kit (Condition #3)

MPP03 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S3

Dilution Preparative Membrane Protein Folding Kit (Condition #4)

MPP04 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S4

Dilution Preparative Membrane Protein Folding Kit (Condition #5)

MPP05 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S5

Dilution Preparative Membrane Protein Folding Kit (Condition #6)

MPP06 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S6

Dilution Preparative Membrane Protein Folding Kit (Condition #7)

MPP07 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S7

Dilution Preparative Membrane Protein Folding Kit (Condition #8)

MPP08 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S8

Dilution Preparative Membrane Protein Folding Kit (Condition #9)

MPP09 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S9

Dilution Preparative Membrane Protein Folding Kit (Condition #10)

MPP10 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S10.

Dilution Preparative Membrane Protein Folding Kit (Condition #11)

MPP11 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S11

Dilution Preparative Membrane Protein Folding Kit (Condition #12)

MPP12 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S12

Dilution Preparative Membrane Protein Folding Kit (Condition #13)

MPP13 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S13

Dilution Preparative Membrane Protein Folding Kit (Condition #14)

MPP14 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S14

Dilution Preparative Membrane Protein Folding Kit (Condition #15)

MPP15 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S15

Dilution Preparative Membrane Protein Folding Kit (Condition #16)

MPP16 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S16

Dilution Preparative Membrane Protein Folding Kit (Condition #17)

MPP17 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S17

Dilution Preparative Membrane Protein Folding Kit (Condition #18)

MPP18 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S18

Dilution Preparative Membrane Protein Folding Kit (Condition #19)

MPP19 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S19

Dilution Preparative Membrane Protein Folding Kit (Condition #20)

MPP20 kit
EUR 242.54
Description: This product includes 0.4 ml of Reagent B, 7.2 ml of Reagent C, 25 ml of 20 Solution S20

Anti-CK-MB Detection Antibody

A2089-100 100 µg
EUR 417.6

Anti-D-Dimer Detection Antibody

A2090-100 100 µg
EUR 405.6

Anti-D-Dimer Detection Antibody

A2091-100 100 µg
EUR 405.6

Anti-Lp-PLA2 Detection Antibody

A2095-100 100 µg
EUR 435.6

Alpha-fetoprotein, AFP (Detection) Antibody

abx019292-1mg 1 mg
EUR 627.6

Senescence Detection Kit

55R-1370 250 tests
EUR 854.4
Description: Senescence Detection Kit for use in the research laboratory

HMGB1 Detection Kit

6010 1 kit
EUR 903.9
Description: HMGB1 Detection Kit

SEB Detection Kit

6030 1 kit
EUR 663.78
Description: SEB Detection Kit

OVA Detection ELISA

6032 1 kit
EUR 663.78
Description: OVA Detection ELISA

NGAL (Detection Ab)

abx019242-100ug 100 ug
EUR 777.6

Detection Reagent A

abx296004-120ul 120 ul
EUR 385.2

Autophagy Detection Kit

abx299014-50tests 50 tests
EUR 627.6

Autophagy Detection Kit

abx299015-200tests 200 tests
EUR 1045.2

Apoptosis Detection Kit

ANXVKB-100T 100 test
EUR 557.64

Apoptosis Detection Kit

ANXVKCFB-100T 100 test
EUR 495.24

Apoptosis Detection Kit

ANXVKCFB7-100T 100 test
EUR 495.24

Apoptosis Detection Kit

ANXVKDY-100T 100 test
EUR 467.16

Apoptosis Detection Kit

ANXVKF-100T 100 test
EUR 395.4

Apoptosis Detection Kit

ANXVKF7-100T 100 test
EUR 395.4

Apoptosis Detection Kit

ANXVKPE-100T 100 test
EUR 415.68

GenomeCoV19 Detection Kit

G628 100 Rxns
EUR 384
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

GenomeCoV19 Detection Kit

G628.v2 100 Rxns
EUR 384
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

Formaldehyde Detection Kit

K001-F1 2x96 well plate
EUR 510

Senescence Detection Kit

K2030-250 250 Staining
EUR 552

Senescence Detection Kit

K320-250
EUR 561.6

IRAK Detection Set

PSI-1802 1 Set
EUR 884.4
Description: Members of the IRAK (IL-1R-associated kinase)/Pelle family play a major role in IL-1R/TLR mediated inflammatory responses and in innate immunity. IRAK and IRAK-2 regulate the activity of a signaling cascade that mediates the activation of NF-κB and MAP kinase. IRAK-4 interacts with and phosphorylates IRAK, while IRAK-M is thought to inhibit the recruitment and activation of IRAK-4 and IRAK. The importance of the IRAK family in inflammation and immunity is illustrated by the fact that animals lacking IRAK-4 are impaired in their responses to viral and bacterial challenges and are completely resistant to LPS challenge.;;For images please see PDF data sheet

TLR Detection Set

PSI-1806 1 Set
EUR 1627.8
Description: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include MyD88, TIRAP, TIRP, TOLLIP, and TRIF. These molecules interact with and activate the IL-1R-associated kinase (IRAK) family, which then activates TNF receptor associated factor (TRAF)-6, and ultimately leads to the activation of NF-κB. While most TLRs utilize more than one adaptor, certain adaptor molecules are essential for individual TLR signaling, e.g., TLR4 signaling is dependent on TIRP expression.;;For images please see PDF data sheet

ORAI Detection Set

PSI-1819 1 Set
EUR 1627.8
Description: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. The ORAI family is a recently identified set of proteins that are essential components of these CRAC channels. A missense mutation in the ORAI1 protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with the stromal interaction molecule 1 (STIM1), the store-activated endoplasmic reticulum Ca++ sensor. Like ORAI1, ORAI2 also functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, although at a lesser efficacy than ORAI1. Although ORAI3 can also function as Ca++ plasma membrane channel, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. STIM1, in its function as a Ca++ sensor and an activator of CRAC channels, migrates to the plasma membrane from endoplasmic reticulum-like sites which act as cellular Ca++ stores. A related molecule, STIM2, inhibits the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting these two proteins may play a coordinated role in controlling Ca++ entry. The ORAI antibodies are predicted to have no cross-reactivity to the other ORAI proteins. Similarly, the STIM antibodies will not cross-react with the other STIM protein.;;For images please see PDF data sheet

Grik Detection Set

PSI-1824 1 Set
EUR 1627.8
Description: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and Grik5/KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Grik2, also known as glutamate receptor 6, may be associated with autosomal recessive mental retardation and possibly other neurological disorders such as schizophrenia. Numerous isoforms of Grik2 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow. Grik3, also known as glutamate receptor 7, has recently been shown to be an essential subunit of presynaptic kainate autoreceptors at hippocampal mossy fiber synapses as grik3-null mice show significantly reduced short- and long-term synaptic potentiation. Grik4 codes for the KA1 subunit of kainate-type ionotropic gluatamate receptors; mutations in this gene show significant association with both schizophrenia and bipolar disorder. Grik5, also known as kainate-preferring glutamate receptor subunit KA2, does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface. ;;For images please see PDF data sheet

SAPAP Detection Set

PSI-1826 1 Set
EUR 752.1
Description: The members of the SAP90/PSD-95-associated protein (SAPAP) family (also known as the DLGAP family) specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1, -2 and -4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). SAPAP3 protein is targeted to dendrites. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. Multiple isoforms of the SAPAP proteins are known to exist.;;For images please see PDF data sheet

Junctophilin Detection Set

PSI-1828 1 Set
EUR 752.1
Description: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1). JPH2-null mice died of embryonic cardiac arrest and human patients with mutations in the JPH2 gene showed hypertrophic cardiomyopathy, demonstrating the importance of this protein. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. Multiple isoforms of the JPH proteins are known to exist.;;For images please see PDF data sheet

Matrillin Detection Set

PSI-1830 1 Set
EUR 752.1
Description: Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). MATN1 and MATN3 are expressed specifically in cartilage and are among the most up-regulated ECM proteins during chondrogenesis. MATN1 is composed of two Willebrand Factor A (vWFA) domains separated by one EGF-like domain, whereas MATN3 is composed of a single N-terminal vWFA domain followed by four epidermal growth factor (EGF) repeats and a coiled-coil domain. MATN1 or MATN3 may play a role in modulating chondrogenesis during the chondrocyte differentiation process. Mutations of MATN1 have been associated with variety of inherited chondrodysplasias, while aberrant expression and processing of MATN3 are hallmarks of conventional cartilaginous neoplasms. The MATN1 promoter region has also been shown to be associated with both susceptibility and disease progression in Adolescent idiopathic scoliosis. Other studies indicate that MATN2 is a permissive substrate for axonal growth and cell migration, and it is required for successful nerve regeneration, while MATN4 could serve as an odontoblast differentiation marker, e.g. in odontoblast stem cell research.;;For images please see PDF data sheet

Ubiquitin Detection Kit

SKT-131-20 20 assays
EUR 594
Description: Purification Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples. in Cell Lysates, Tissue samples from all species

Glutathione Detection Kit

SKT-202-96 1 plate of 96 wells
EUR 513.6
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species

PDL1 Detection Set

SD8500 1 Set
EUR 355.2
Description: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.

PD1 Detection Set

SD8600 1 Set
EUR 537.9
Description: PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

LAG3 Detection Set

SD8700 1 Set
EUR 569.4
Description: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

TIGIT Detection Set

SD8800 1 Set
EUR 569.4
Description: TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Ecl Detection Reagents

RPN2105 EACH
EUR 611.04

Ecl Detection Reagents

RPN3004 EACH
EUR 489.06

Calcium calibration buffer

59100 1KIT
EUR 261.6
Description: Minimum order quantity: 1 unit of 1KIT

TBE Buffer, 5X

41006 4L
EUR 133.2
Description: Minimum order quantity: 1 unit of 4L

Propidium Iodide Buffer

40048 2ML
EUR 141.6
Description: Minimum order quantity: 1 unit of 2ML

AEC Substrate Buffer

2123-50
EUR 164.4

Cell Lysis Buffer

1067-100
EUR 196.8

Cell Lysis Buffer

1067-400
EUR 352.8

2X Reaction Buffer

1068-20
EUR 222

2X Reaction Buffer

1068-80
EUR 352.8

IMAC phosphate buffer

09-1010-10 10 tablets/ blisterpack
EUR 94.8

IMAC phosphate buffer

09-1010-50 50 tablets
EUR 139.2

IMAC elution buffer

09-1012-10 10 tablets/ blisterpack
EUR 102

IMAC elution buffer

09-1012-50 50 tablets
EUR 201.6

Wash Buffer (25X)

abx092104-30ml 30 ml
EUR 126

DNA Loading Buffer

abx098143-5ml 5 ml
EUR 210

2xRNA Loading Buffer

abx098243-1ml 1 ml
EUR 477.6

Standard Diluent Buffer

abx098951-20ml 20 ml
EUR 109.2

Sample Diluent Buffer

abx296001-20ml 20 ml
EUR 109.2

Diluent Buffer A

abx296005-10ml 10 ml
EUR 109.2

Hepes Buffer 99.5%

AK1069-0250 250g Ask for price

Hepes Buffer 99.5%

AK1069-0500 500g Ask for price

Hepes Buffer 99.5%

AK1069-1000 1kg Ask for price

5X RIPA Buffer

AKR-191 20mL
EUR 164.4
Description: RIPA Buffer extracts proteins from cultured mammalian cells for various downstream applications.

10x Taq Buffer

BA00151 1.8ml
EUR 66
Description: High purity buffer for various PCR applications.

NH4 Buffer, 10x

BIO-37025 3 x 1.2ml Ask for price

Citrate Buffer Powder

AR0024 2000mL/pack
EUR 63.6

Cell Lysis Buffer

AR0103 100mL (The kit contains sufficient lysis buffer for 200 cell pellet fractions containing 5x 106 cells each)
EUR 145.2

RIPA Lysis Buffer

AR0105 50 mL
EUR 90
Conclusions: To sum up, these outcomes proposed that XIST functioned as an endogenous sponge in selling PC cell proliferation by means of competing for miR-137 to manage Notch1 expression, and will present extra therapeutic targets for the sufferers with PC.