SET7/9 promotes H3K4me3 at lncRNA DRAIC promoter to modulate growth and metastasis of glioma

Goal: We geared toward investigating the expression ranges of SET7/9 in glioma and the connection between SET7/9 and LncRNA DRAIC. Additional, we explored the connection between SET7/9 and glioma cell metastasis and temper.

Sufferers and strategies: The expression ranges of DRAIC and miR-18a-3p in gastric most cancers cells have been measured by quantitative polymerase chain response (qPCR). The binding website of the promoter of DRAIC by H3K4me3 was confirmed by ChIP-Actual-time PCR. The direct goal of DRAIC and miR-18a-3p in gastric most cancers cells was measured by a Luciferase reporter assay. Cell proliferation was detected by Cell counting package-8 (CCK8), and cell invasion and migration have been measured by transwell assays.

Outcomes: In contrast with adjoining non-cancerous regular tissues, SET7/9 and DRAIC have been each downregulated and miR-18a-3p was upregulated in glioma cells. In the meantime, silencing of SET7/9 enhanced cell proliferation, migration, and invasion in U251 cells. H3K4me3 protein can bind to DRAIC promoter immediately. Inhibition of SET7/9 and downregulation of DRAIC in U251 cells reversed the impact of SET7/9 silencing on the expansion and metastasis of glioma cells. In U251 cells, SET7/9 and DRAIC overexpression inhibited cell proliferation, migration and invasion. As well as, miR-18a-3p interacts with DRAIC by means of direct binding. The inhibition of DRAIC promoted the expansion and metastasis of U251 cells, whereas the co-transfection of si- DRAIC and miR-18a-3p additional promoted the expansion and metastasis of U251 cells. Overexpression of DRAIC inhibited the expansion and metastasis of cells, utterly reversing the co-transfection of Lnc-DRAIC and miR-18a-3p.

Conclusions: On this analysis, we found that the expression of SET7/9 was low in glioma cells and SET7/9-mediated H3K4me3 enrichment on the DRAIC promoter regulated the expansion and metastasis of glioma cells by concentrating on miR-18a-3p. It doubtlessly supplies a brand new therapeutic marker concentrating on glioma.

MicroRNA (miR)-335-5P has the power to manage chondrogenic differentiation and promote chondrogenesis in mouse mesenchymal stem cells. Additionally it is abnormally elevated in human osteoarthritic chondrocytes. Nevertheless, the organic perform of miR-335-5P in osteoarthritis (OA) isn’t effectively understood. The current examine investigated the mechanism of miR-335-5P within the pathogenesis of OA. To analyze the impact of miR-335-5P on the pathogenesis of OA in vitro, a miR-335-5P mimic and inhibitor have been transfected into chondrocytes.

Cell Counting package-Eight assay and circulate cytometry have been used to observe the results of miR-335-5P on chondrocyte apoptosis and the expression of cartilage-specific genes, equivalent to aggrecan, collagen II, matrix metalloproteinase 13 and collagen X, have been detected by reverse transcription-quantitative PCR and western blot evaluation. Furthermore, the present examine assessed whether or not HMG-box transcription issue 1 (HBP1) is a novel goal of miR-335-5P with twin luciferase reporter assays. Lastly, a rescue experiment was used to show the regulation between miR-335-5P and HBP1.

The outcomes revealed that HBP1 was a novel goal of miR-335-5P, and that miR-335-5P mediated the apoptosis of chondrocytes and modifications in cartilage-specific genes by way of concentrating on HBP1. Total, the current examine revealed that miR-335-5P mediated the event of OA by concentrating on the HBP1 gene and selling chondrocyte apoptosis. These knowledge advised that miR-335-5P could also be used to develop novel early-stage diagnostic and therapeutic methods for OA.

IgE Dilution Buffer
3009	Chondrex	1 mg/ml x 10 ml	EUR 96.54
Description: IgE Dilution Buffer

Library Dilution Buffer
abx098898-5ml	Abbexa	5 ml	EUR 309.6

Library Dilution Buffer
NQ106	Vazyme	50 ml	EUR 188.4

Topoisomerase II Dilution Buffer
TG4090	TopoGen	1ml	EUR 187.2

Topoisomerase I Dilution Buffer
TG4095	TopoGen	1ml	EUR 187.2

ChonBlock Western Blot Buffer 1 (PBS)
9069P	Chondrex	10 ml	EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)
9069T	Chondrex	10 ml	EUR 232.26
Description: ChonBlock Western Blot Buffer 1 (TBS)

Dilution buffer R (10x); ELISPOT
CT348	U-CyTech	10 ml	EUR 93.6

Dilution buffer T (10x); ELISPOT
CT358	U-CyTech	8 ml	EUR 93.6

Antibody Dilution Buffer for ICC and IHC
SF40010	Neuromics	10 ml	EUR 146.4

Dilution Buffer [1x PBS pH 7.3]
0209B	AthenaES	100 ml	EUR 92.4

Dilution buffer B (10x); ELISPOT (PVDF)
CT364	U-CyTech	8 ml	EUR 93.6

4x Detection Buffer
79389	BPS Bioscience	10 ml	EUR 195
Description: The 4x Detection Buffer is optimized for use with the PRMT5 Homogeneous Assay Kit (BPS Bioscience #52052).

ChonBlock Western Blot Buffer 2 for Goat (PBS)
90691P	Chondrex	10 ml	EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (PBS)

ChonBlock Western Blot Buffer 2 for Goat (TBS)
90691T	Chondrex	10 ml	EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Goat (TBS)

ChonBlock Western Blot Buffer 2 for Rabbit (PBS)
90692P	Chondrex	10 ml	EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (PBS)

ChonBlock Western Blot Buffer 2 for Rabbit (TBS)
90692T	Chondrex	10 ml	EUR 145.26
Description: ChonBlock Western Blot Buffer 2 for Rabbit (TBS)

West-Ezier Antibody Dilution Buffer, ready-to-use
W3872-050	GenDepot	500ml	EUR 198

4x DMT Detection Buffer 1
52301	BPS Bioscience	10 ml	EUR 210
Description: The 4x Demethyltransferase Detection Buffer 1 is optimized for use with the following BPS Bioscience JMJD2 assay kits: ; • JMJD2A Homogeneous Assay Kit, #50413; • JMJD2B Homogeneous Assay Kit, #50414; • JMJD2C Homogeneous Assay Kit, #50415; • JMJD2E Homogeneous Assay Kit, #50417; • JMJD2C Chemiluminescent Assay Kit, #50405; • LSD1 Homogeneous Assay Kit, #50108.

3x Homogeneous BRD Detection Buffer 1
33002	BPS Bioscience	10 ml	EUR 195
Description: The 3x BRD Detection Buffer 1 is optimized for use with the following BPS Bioscience assay kits:_x000D_• BRD2 (BD2) Inhibitor Screening Assay Kit #32522 384 reactions • BRD3 (BD2) Inhibitor Screening Assay Kit #32523 384 reactions • BRD3 (BD1) Inhibitor Screening Assay Kit #32513 384 reactions • BRD4 (BD1) Inhibitor Screening Assay Kit #32514 384 reactions • BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions_x000D_NOTE: This product was formerly known as 3x BRD Detection Buffer.

3x BRD Homogeneous Detection Buffer 2
33006	BPS Bioscience	10 ml	EUR 195
Description: The 3x BRD Homogeneous Detection Buffer 2 is optimized for use with the following BPS Bioscience assay kits: • ATAD2A Inhibitor Screening Assay Kit #32601 384 reactions • ATAD2B Inhibitor Screening Assay Kit #32605 384 reactions • BRD1 Inhibitor Screening Assay Kit #32521 384 reactions • BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions • BRD9 (BD1) Inhibitor Screening Assay Kit #32519 384 reactions • BRPF3 Inhibitor Screening Assay Kit #32608 384 reactions • CECR2 Inhibitor Screening Assay Kit #32611 384 reactions • CREBBP Inhibitor Screening Assay Kit #32607 384 reactions • SMARCA2 Inhibitor Screening Assay Kit #32610 384 reactions • SMARCA4/BRG1 Inhibitor Screening Assay Kit #32609 384 reactions • TAF1 (BD1/BD2) Inhibitor Screening Assay Kit #32604 384 reactions • TAF1L (BD2) Inhibitor Screening Assay Kit #32602 384 reactions • TAF1L (BD1/BD2) Inhibitor Screening Assay Kit #32603 384 reactions • TRIM24 Inhibitor Screening Assay Kit #32606 384 reactions_x000D_NOTE: This product was formerly named 3x ATAD2A Detection Buffer.

3x BRD Homogeneous Detection Buffer 3
33015	BPS Bioscience	10 ml	EUR 195
Description: The 3x BRD Homogeneous Detection Buffer 3 is optimized for use with the following BPS Bioscience assay kits:_x000D_• BAZ2B Inhibitor Screening Assay Kit #32600 384 reactions_x000D_NOTE: This product was formerly known as BAZ2B Detection Buffer.

HCl Dilution Matrix (10g/l HCl)
HADM1	Scientific Laboratory Supplies	1L	EUR 107.16

HCl Dilution Matrix 10g/L HCl
HADM1A	Scientific Laboratory Supplies	2ML	EUR 98.04

Kinesis Blood Dilution Vial; 25ml Bulk
CHR1062	Scientific Laboratory Supplies	PK1000	EUR 159.13

Dilution Membrane Protein Folding Screen Kit
MPS01-20	ProFoldin	kit	EUR 117.02
Description: This product includes 0.1 ml of Reagent A, 0.1 ml of Reagent B, 1.1 ml of Reagent C, 0.35 ml of 20 Solutions 1-20.

TnI (Detection) Antibody
abx018057-100ug	Abbexa	100 ug	EUR 410.4

CK19 (Detection) Antibody
abx019246-1mg	Abbexa	1 mg	EUR 794.4

PCT (Detection) Antibody
20-abx019248	Abbexa	EUR 469.20 EUR 744.00	0.5 mg 1 mg

Antibody diluent buffer
AR1106-2	BosterBio	12ml	EUR 109.2

Anti-ST2 Detection Antibody
A2099-100	Biovision	100 µg	EUR 435.6

PCT (Detection) Antibody (Biotin)
abx019264-1mg	Abbexa	1 mg	EUR 1078.8

Albumin (Capture / Detection) Antibody
abx019293-1mg	Abbexa	1 mg	EUR 661.2

Albumin (Capture / Detection) Antibody
abx019294-1mg	Abbexa	1 mg	EUR 661.2

Fetal Fibronectin (Detection) Antibody
abx019300-1mg	Abbexa	1 mg	EUR 744

Fetal Fibronectin (Detection) Antibody
abx019302-1mg	Abbexa	1 mg	EUR 744

SCCA1 (Capture / Detection) Antibody
abx019308-1mg	Abbexa	1 mg	EUR 794.4

SCCA1 (Capture / Detection) Antibody
abx019309-1mg	Abbexa	1 mg	EUR 794.4

WB / Antibody Stripping Buffer
20-abx090656	Abbexa	EUR 226.80 EUR 276.00	100 ml 200 ml

WB / Antibody Stripping Buffer
abx090671-200ml	Abbexa	200 ml	EUR 226.8

Tricine buffer
20-abx082551	Abbexa	EUR 260.40 EUR 226.80	100 g 25 g

Wash Buffer
abx098952-20ml	Abbexa	20 ml	EUR 109.2

Coating Buffer
abx098970-1vial	Abbexa	1 vial	EUR 184.8

Blocking Buffer
abx098972-1vial	Abbexa	1 vial	EUR 184.8

Lysis Buffer
abx098984-LysisBuffer120ml	Abbexa	Lysis Buffer 1 (20 ml)	EUR 184.8

Lysis Buffer
abx098984-LysisBuffer3100ml	Abbexa	Lysis Buffer 3 (100ml)	EUR 276

Lysis Buffer
abx098984-LysisBuffer420ml	Abbexa	Lysis Buffer 4 (20 ml)	EUR 184.8

Binding Buffer
abx290019-50ml	Abbexa	50 ml	EUR 276

Wash Buffer
abx293002-30ml	Abbexa	30 ml	EUR 126

Binding Buffer
BB10X-50ML	ImmunoStep	50 ML	EUR 136.44

ADA buffer
AD0003	Bio Basic	25g	EUR 77.75

Wash Buffer
KF17356	Neuromics	500 ml	EUR 214.8

RIPA Buffer
GR103019	Genorise Scientific	50 mL	EUR 70.8

Stripping Buffer
GR103020	Genorise Scientific	500 mL	EUR 106.8

Anode Buffer
GR103036	Genorise Scientific	1 L	EUR 106.8

Cathode Buffer
GR103037	Genorise Scientific	1 L	EUR 130.8

10xTaq Buffer
PCRB60	Bio Basic	4x1.5ml, 6ml	EUR 70.44

PBS Buffer
RM00012	Abclonal	2L	EUR 122.4

Phosphate Buffer
PB1	Scientific Laboratory Supplies	1L	EUR 94.62

Wash Buffer
1210-200	Biovision	each	EUR 235.2

RIPA Buffer
2114-100	Biovision	each	EUR 189.6

RIPA Buffer
2114-500	Biovision	each	EUR 555.6

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-783-CM	CORNING	1 L /pk	EUR 75.6
Description: Specialty Media; Islet products

Dilution Preparative Membrane Protein Folding Kit (Condition #1)
MPP01	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S1.

Dilution Preparative Membrane Protein Folding Kit (Condition #2)
MPP02	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S2

Dilution Preparative Membrane Protein Folding Kit (Condition #3)
MPP03	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S3

Dilution Preparative Membrane Protein Folding Kit (Condition #4)
MPP04	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S4

Dilution Preparative Membrane Protein Folding Kit (Condition #5)
MPP05	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S5

Dilution Preparative Membrane Protein Folding Kit (Condition #6)
MPP06	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S6

Dilution Preparative Membrane Protein Folding Kit (Condition #7)
MPP07	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S7

Dilution Preparative Membrane Protein Folding Kit (Condition #8)
MPP08	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S8

Dilution Preparative Membrane Protein Folding Kit (Condition #9)
MPP09	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S9

Dilution Preparative Membrane Protein Folding Kit (Condition #10)
MPP10	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S10.

Dilution Preparative Membrane Protein Folding Kit (Condition #11)
MPP11	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S11

Dilution Preparative Membrane Protein Folding Kit (Condition #12)
MPP12	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S12

Dilution Preparative Membrane Protein Folding Kit (Condition #13)
MPP13	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S13

Dilution Preparative Membrane Protein Folding Kit (Condition #14)
MPP14	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S14

Dilution Preparative Membrane Protein Folding Kit (Condition #15)
MPP15	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S15

Dilution Preparative Membrane Protein Folding Kit (Condition #16)
MPP16	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S16

Dilution Preparative Membrane Protein Folding Kit (Condition #17)
MPP17	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S17

Dilution Preparative Membrane Protein Folding Kit (Condition #18)
MPP18	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S18

Dilution Preparative Membrane Protein Folding Kit (Condition #19)
MPP19	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent A, 7.2 ml of Reagent C, 25 ml of 20 Solution S19

Dilution Preparative Membrane Protein Folding Kit (Condition #20)
MPP20	ProFoldin	kit	EUR 242.54
Description: This product includes 0.4 ml of Reagent B, 7.2 ml of Reagent C, 25 ml of 20 Solution S20

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers
5730	Scientific Laboratory Supplies	EACH	EUR 864.12

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-050	GenDepot	500ml	EUR 96

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-100	GenDepot	2X500ml	EUR 124.8

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-101	GenDepot	1L	EUR 114

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-200	GenDepot	4X500ml	EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-201	GenDepot	2X1L	EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-401	GenDepot	4X1L	EUR 198

TT Buffer (Tris-Tricine buffer) Primix powder
TD8133	Bio Basic	1PK, 10L	EUR 91.32

Anti-CK-MB Detection Antibody
A2089-100	Biovision	100 µg	EUR 417.6

Anti-D-Dimer Detection Antibody
A2090-100	Biovision	100 µg	EUR 405.6

Anti-D-Dimer Detection Antibody
A2091-100	Biovision	100 µg	EUR 405.6

Anti-Lp-PLA2 Detection Antibody
A2095-100	Biovision	100 µg	EUR 435.6

Alpha-fetoprotein, AFP (Detection) Antibody
abx019292-1mg	Abbexa	1 mg	EUR 627.6

Senescence Detection Kit
55R-1370	Fitzgerald	250 tests	EUR 854.4
Description: Senescence Detection Kit for use in the research laboratory

HMGB1 Detection Kit
6010	Chondrex	1 kit	EUR 903.9
Description: HMGB1 Detection Kit

SEB Detection Kit
6030	Chondrex	1 kit	EUR 663.78
Description: SEB Detection Kit

OVA Detection ELISA
6032	Chondrex	1 kit	EUR 663.78
Description: OVA Detection ELISA

NGAL (Detection Ab)
abx019242-100ug	Abbexa	100 ug	EUR 777.6

Detection Reagent A
abx296004-120ul	Abbexa	120 ul	EUR 385.2

Autophagy Detection Kit
abx299014-50tests	Abbexa	50 tests	EUR 627.6

Autophagy Detection Kit
abx299015-200tests	Abbexa	200 tests	EUR 1045.2

Apoptosis Detection Kit
ANXVKB-100T	ImmunoStep	100 test	EUR 557.64

Apoptosis Detection Kit
ANXVKCFB-100T	ImmunoStep	100 test	EUR 495.24

Apoptosis Detection Kit
ANXVKCFB7-100T	ImmunoStep	100 test	EUR 495.24

Apoptosis Detection Kit
ANXVKDY-100T	ImmunoStep	100 test	EUR 467.16

Apoptosis Detection Kit
ANXVKF-100T	ImmunoStep	100 test	EUR 395.4

Apoptosis Detection Kit
ANXVKF7-100T	ImmunoStep	100 test	EUR 395.4

Apoptosis Detection Kit
ANXVKPE-100T	ImmunoStep	100 test	EUR 415.68

GenomeCoV19 Detection Kit
G628	ABM	100 Rxns	EUR 384
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

GenomeCoV19 Detection Kit
G628.v2	ABM	100 Rxns	EUR 384
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

Formaldehyde Detection Kit
K001-F1	Arbor Assays	2x96 well plate	EUR 510

Senescence Detection Kit
K2030-250	ApexBio	250 Staining	EUR 552

IRAK Detection Set
PSI-1802	ProSci	1 Set	EUR 884.4
Description: Members of the IRAK (IL-1R-associated kinase)/Pelle family play a major role in IL-1R/TLR mediated inflammatory responses and in innate immunity. IRAK and IRAK-2 regulate the activity of a signaling cascade that mediates the activation of NF-κB and MAP kinase. IRAK-4 interacts with and phosphorylates IRAK, while IRAK-M is thought to inhibit the recruitment and activation of IRAK-4 and IRAK. The importance of the IRAK family in inflammation and immunity is illustrated by the fact that animals lacking IRAK-4 are impaired in their responses to viral and bacterial challenges and are completely resistant to LPS challenge.;;For images please see PDF data sheet

TLR Detection Set
PSI-1806	ProSci	1 Set	EUR 1627.8
Description: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include MyD88, TIRAP, TIRP, TOLLIP, and TRIF. These molecules interact with and activate the IL-1R-associated kinase (IRAK) family, which then activates TNF receptor associated factor (TRAF)-6, and ultimately leads to the activation of NF-κB. While most TLRs utilize more than one adaptor, certain adaptor molecules are essential for individual TLR signaling, e.g., TLR4 signaling is dependent on TIRP expression.;;For images please see PDF data sheet

ORAI Detection Set
PSI-1819	ProSci	1 Set	EUR 1627.8
Description: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. The ORAI family is a recently identified set of proteins that are essential components of these CRAC channels. A missense mutation in the ORAI1 protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with the stromal interaction molecule 1 (STIM1), the store-activated endoplasmic reticulum Ca++ sensor. Like ORAI1, ORAI2 also functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, although at a lesser efficacy than ORAI1. Although ORAI3 can also function as Ca++ plasma membrane channel, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. STIM1, in its function as a Ca++ sensor and an activator of CRAC channels, migrates to the plasma membrane from endoplasmic reticulum-like sites which act as cellular Ca++ stores. A related molecule, STIM2, inhibits the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting these two proteins may play a coordinated role in controlling Ca++ entry. The ORAI antibodies are predicted to have no cross-reactivity to the other ORAI proteins. Similarly, the STIM antibodies will not cross-react with the other STIM protein.;;For images please see PDF data sheet

Grik Detection Set
PSI-1824	ProSci	1 Set	EUR 1627.8
Description: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and Grik5/KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Grik2, also known as glutamate receptor 6, may be associated with autosomal recessive mental retardation and possibly other neurological disorders such as schizophrenia. Numerous isoforms of Grik2 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow. Grik3, also known as glutamate receptor 7, has recently been shown to be an essential subunit of presynaptic kainate autoreceptors at hippocampal mossy fiber synapses as grik3-null mice show significantly reduced short- and long-term synaptic potentiation. Grik4 codes for the KA1 subunit of kainate-type ionotropic gluatamate receptors; mutations in this gene show significant association with both schizophrenia and bipolar disorder. Grik5, also known as kainate-preferring glutamate receptor subunit KA2, does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface. ;;For images please see PDF data sheet

SAPAP Detection Set
PSI-1826	ProSci	1 Set	EUR 752.1
Description: The members of the SAP90/PSD-95-associated protein (SAPAP) family (also known as the DLGAP family) specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1, -2 and -4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). SAPAP3 protein is targeted to dendrites. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. Multiple isoforms of the SAPAP proteins are known to exist.;;For images please see PDF data sheet

Junctophilin Detection Set
PSI-1828	ProSci	1 Set	EUR 752.1
Description: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1). JPH2-null mice died of embryonic cardiac arrest and human patients with mutations in the JPH2 gene showed hypertrophic cardiomyopathy, demonstrating the importance of this protein. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. Multiple isoforms of the JPH proteins are known to exist.;;For images please see PDF data sheet

Matrillin Detection Set
PSI-1830	ProSci	1 Set	EUR 752.1
Description: Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). MATN1 and MATN3 are expressed specifically in cartilage and are among the most up-regulated ECM proteins during chondrogenesis. MATN1 is composed of two Willebrand Factor A (vWFA) domains separated by one EGF-like domain, whereas MATN3 is composed of a single N-terminal vWFA domain followed by four epidermal growth factor (EGF) repeats and a coiled-coil domain. MATN1 or MATN3 may play a role in modulating chondrogenesis during the chondrocyte differentiation process. Mutations of MATN1 have been associated with variety of inherited chondrodysplasias, while aberrant expression and processing of MATN3 are hallmarks of conventional cartilaginous neoplasms. The MATN1 promoter region has also been shown to be associated with both susceptibility and disease progression in Adolescent idiopathic scoliosis. Other studies indicate that MATN2 is a permissive substrate for axonal growth and cell migration, and it is required for successful nerve regeneration, while MATN4 could serve as an odontoblast differentiation marker, e.g. in odontoblast stem cell research.;;For images please see PDF data sheet

Ubiquitin Detection Kit
SKT-131-20	Stressmarq	20 assays	EUR 594
Description: Purification Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples. in Cell Lysates, Tissue samples from all species

Glutathione Detection Kit
SKT-202-96	Stressmarq	1 plate of 96 wells	EUR 513.6
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species

PDL1 Detection Set
SD8500	ProSci	1 Set	EUR 355.2
Description: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.

PD1 Detection Set
SD8600	ProSci	1 Set	EUR 537.9
Description: PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

LAG3 Detection Set
SD8700	ProSci	1 Set	EUR 569.4
Description: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

TIGIT Detection Set
SD8800	ProSci	1 Set	EUR 569.4
Description: TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Ecl Detection Reagents
RPN2105	Scientific Laboratory Supplies	EACH	EUR 611.04

Ecl Detection Reagents
RPN3004	Scientific Laboratory Supplies	EACH	EUR 489.06

Senescence Detection Kit
K320-250	Biovision	each	EUR 561.6

Mouse IgG Isotype control; Concentrate Minimum 1:50 dilution
NG903C	Innovex	1 ml	EUR 373.2

Calcium calibration buffer
59100	Biotium	1KIT	EUR 261.6
Description: Minimum order quantity: 1 unit of 1KIT

TBE Buffer, 5X
41006	Biotium	4L	EUR 133.2
Description: Minimum order quantity: 1 unit of 4L

IMAC phosphate buffer
09-1010-10	Medicago	10 tablets/ blisterpack	EUR 94.8

IMAC phosphate buffer
09-1010-50	Medicago	50 tablets	EUR 139.2

IMAC elution buffer
09-1012-10	Medicago	10 tablets/ blisterpack	EUR 102

IMAC elution buffer
09-1012-50	Medicago	50 tablets	EUR 201.6

Wash Buffer (25X)
abx092104-30ml	Abbexa	30 ml	EUR 126

DNA Loading Buffer
abx098143-5ml	Abbexa	5 ml	EUR 210

2xRNA Loading Buffer
abx098243-1ml	Abbexa	1 ml	EUR 477.6

Standard Diluent Buffer
abx098951-20ml	Abbexa	20 ml	EUR 109.2

Sample Diluent Buffer
abx296001-20ml	Abbexa	20 ml	EUR 109.2

Diluent Buffer A
abx296005-10ml	Abbexa	10 ml	EUR 109.2

Hepes Buffer 99.5%
AK1069-0250	Akron Biotech	250g	Ask for price

Hepes Buffer 99.5%
AK1069-0500	Akron Biotech	500g	Ask for price

Hepes Buffer 99.5%
AK1069-1000	Akron Biotech	1kg	Ask for price

5X RIPA Buffer
AKR-191	Cell Biolabs	20mL	EUR 164.4
Description: RIPA Buffer extracts proteins from cultured mammalian cells for various downstream applications.

10x Taq Buffer
BA00151	Bioatlas	1.8ml	EUR 66
Description: High purity buffer for various PCR applications.

NH4 Buffer, 10x
BIO-37025	Bioline	3 x 1.2ml	Ask for price

Citrate Buffer Powder
AR0024	BosterBio	2000mL/pack	EUR 63.6

Cell Lysis Buffer
AR0103	BosterBio	100mL (The kit contains sufficient lysis buffer for 200 cell pellet fractions containing 5x 106 cells each)	EUR 145.2

RIPA Lysis Buffer
AR0105	BosterBio	50 mL	EUR 90