EnDuo, a novel derivative of Endostar, inhibits the migration of colon cancer cells, suppresses matrix metalloproteinase-2/9 expression and impedes AKT/ERK activation

Goal: This research was designed to research the position of microRNA-198 in thyroid most cancers (TCa) development.

Sufferers and strategies: Quantitative real-time polymerase chain response (qRT-PCR) was carried out to look at microRNA-198 and H3F3A ranges in tumor tissue specimens and paracancerous ones collected from 50 sufferers with TCa, and the interaction between microRNA-198 or H3F3A and a few medical indicators or prognosis of TCa sufferers was analyzed as effectively. MicroRNA-198 and H3F3A overexpression fashions had been constructed utilizing lentivirus in TCa cell strains TPC-1 and BHP2-7, and the impacts of microRNA-198 on TCa cell features had been evaluated by utilizing cell counting equipment-8 (CCK-8), plate clone formation, and transwell assays. Lastly, restoration investigations had been carried out to discover the underlying mechanisms in addition to the interplay between microRNA-198 and H3F3A.

Outcomes: QRT-PCR indicated that in tumor tissues of TCa sufferers, microRNA-198 confirmed a remarkably decrease expression than in adjoining regular tissue samples. In contrast with sufferers with excessive expression of microRNA-198, these with microRNA-198 low expression had extra superior tumor stage, bigger tumor measurement, increased lymph node metastasis price, and decrease general survival price. In the meantime, the outcomes of analysis on H3F3A had been simply reverse to the above observations on microRNA-198. In in vitro cell experiments, overexpression of microRNA-198 considerably weakened the proliferation and migration means of thyroid tumor cells.

Annexin V Apoptosis Detection Kit (PE)
abx290015-200test	Abbexa	200 test	EUR 644.4

Annexin V-PE Apoptosis Detection Kit
K128-100	Biovision	each	EUR 601.2

Annexin V-PE Apoptosis Detection Kit
K128-25	Biovision	each	EUR 248.4

Annexin V-PE Apoptosis Detection Kit
K128-400	Biovision	each	EUR 1149.6

Annexin V-PE Apoptosis Detection Kit
K2200-100	ApexBio	100 assays	EUR 619.2

Annexin V-PE Apoptosis Detection Kit
K2200-25	ApexBio	25 assays	EUR 285.6

Annexin V-PE Apoptosis Detection Kit
K2200-400	ApexBio	400 assays	EUR 1336.8

Annexin V Apoptosis Detection Kit (PE) (CY5)
55R-1289	Fitzgerald	25 assays	EUR 416.4
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit Plus (PE)
55R-1304	Fitzgerald	25 assays	EUR 363.6
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V / 7AAD Apoptosis Detection Kit (PE)
abx290009-100test	Abbexa	100 test	EUR 594

Annexin V Apoptosis Detection Kit : PE (OKTA00005)
OKTA00005	Aviva Systems Biology	KIT	EUR 364.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Annexin V-PE-Cy5 Apoptosis Detection Kit
K129-100	Biovision	each	EUR 588

Annexin V-PE-Cy5 Apoptosis Detection Kit
K129-25	Biovision	each	EUR 301.2

Annexin V-PE-Cy5 Apoptosis Detection Kit
K129-400	Biovision	each	EUR 1246.8

Annexin V-PE-Cy5 Apoptosis Detection Kit
K2201-100	ApexBio	100 assays	EUR 668.4

Annexin V-PE-Cy5 Apoptosis Detection Kit
K2201-25	ApexBio	25 assays	EUR 351.6

Annexin V-PE-Cy5 Apoptosis Detection Kit
K2201-400	ApexBio	400 assays	EUR 1503.6

Annexin V Apoptosis Detection Kit (CY3)
55R-1266	Fitzgerald	25 assays	EUR 332.4
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (CY5)
55R-1267	Fitzgerald	25 assays	EUR 332.4
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (FITC)
55R-1265	Fitzgerald	25 assays	EUR 332.4
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (FITC)
abx290002-100test	Abbexa	100 test	EUR 644.4

Annexin V Apoptosis Detection Kit (FITC)
abx290014-200test	Abbexa	200 test	EUR 577.2

Annexin V (PE) / 7-AAD Apoptosis Detection Kit (PE)
abx090609-100tests	Abbexa	100 tests	EUR 718.8

Annexin V Apoptosis Detection Kit (biotin)
55R-1271	Fitzgerald	25 assays	EUR 332.4
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (Biotin)
abx290017-200test	Abbexa	200 test	EUR 744

Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-01	Vazyme	50 rxn	EUR 286.8

Annexin V-PE/7-AAD Apoptosis Detection Kit
A213-02	Vazyme	100 rxn	EUR 369.6

Annexin V EGFP Apoptosis Detection Kit
55R-1268	Fitzgerald	25 assays	EUR 332.4
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V / PI Apoptosis Detection Kit (FITC)
abx290007-100test	Abbexa	100 test	EUR 543.6

Annexin V Apoptosis Detection Kit Plus (CY3)
55R-1303	Fitzgerald	25 assays	EUR 355.2
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit Plus (FITC)
55R-1302	Fitzgerald	25 assays	EUR 355.2
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V / PI Apoptosis Detection Kit (Biotin)
abx290011-100test	Abbexa	100 test	EUR 744

Annexin V Apoptosis Detection Kit (CF-Blue)
abx290018-200test	Abbexa	200 test	EUR 744

Annexin V Apoptosis Detection Kit (Dylight 634)
abx290016-200test	Abbexa	200 test	EUR 644.4

Annexin V (FITC) / PI Apoptosis Detection Kit
abx090601-100tests	Abbexa	100 tests	EUR 552

Annexin V-FITC/PI Apoptosis Detection Kit
A211-01	Vazyme	50 rxn	EUR 259.2

Annexin V-FITC/PI Apoptosis Detection Kit
A211-02	Vazyme	100 rxn	EUR 354

Annexin V Apoptosis Detection Kit : APC (OKTA00002)
OKTA00002	Aviva Systems Biology	KIT	EUR 396
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Annexin V / 7ADD Apoptosis Detection Kit (FITC)
abx290008-100test	Abbexa	100 test	EUR 543.6

Annexin V Apoptosis Detection Kit : FITC (OKTA00003)
OKTA00003	Aviva Systems Biology	KIT	EUR 380.4
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Annexin V / PI Apoptosis Detection Kit (CF-Blue)
abx290012-100test	Abbexa	100 test	EUR 678

Annexin V / PI Apoptosis Detection Kit (Dylight 634)
abx290010-100test	Abbexa	100 test	EUR 644.4

Annexin V (APC) / PI Apoptosis Detection Kit (APC)
abx090605-100tests	Abbexa	100 tests	EUR 777.6

Annexin V (FITC) / 7-AAD Apoptosis Detection Kit
abx090603-100tests	Abbexa	100 tests	EUR 594

Annexin V-FITC / PI Cell Apoptosis Detection Kit
20-abx098246	Abbexa	EUR 292.80 EUR 376.80	25 rxns 50 rxns

Annexin V-EGFP / PI Cell Apoptosis Detection Kit
20-abx098247	Abbexa	EUR 292.80 EUR 376.80	25 rxns 50 rxns

Annexin V / 7AAD Apoptosis Detection Kit (CF-Blue)
abx290013-100test	Abbexa	100 test	EUR 678

Annexin V (APC) / 7-AAD Apoptosis Detection Kit (APC)
abx090607-100tests	Abbexa	100 tests	EUR 811.2

CF594 TUNEL Assay Apoptosis Detection Kit
30064	Biotium	1KIT	EUR 655.2
Description: Minimum order quantity: 1 unit of 1KIT

CF640R TUNEL Assay Apoptosis Detection Kit
30074	Biotium	1KIT	EUR 655.2
Description: Minimum order quantity: 1 unit of 1KIT

CF488A TUNEL Assay Apoptosis Detection Kit
30063	Biotium	1KIT	EUR 655.2
Description: Minimum order quantity: 1 unit of 1KIT

Apoptosis Detection Kit
ANXVKB-100T	ImmunoStep	100 test	EUR 557.64

Apoptosis Detection Kit
ANXVKCFB-100T	ImmunoStep	100 test	EUR 495.24

Apoptosis Detection Kit
ANXVKCFB7-100T	ImmunoStep	100 test	EUR 495.24

Apoptosis Detection Kit
ANXVKDY-100T	ImmunoStep	100 test	EUR 467.16

Apoptosis Detection Kit
ANXVKF-100T	ImmunoStep	100 test	EUR 395.4

Apoptosis Detection Kit
ANXVKF7-100T	ImmunoStep	100 test	EUR 395.4

Apoptosis Detection Kit
ANXVKPE-100T	ImmunoStep	100 test	EUR 415.68

MitoStep + Apoptosis Detection PE
KMAPE-100T	ImmunoStep	100 test	EUR 551.4

MitoStep + Apoptosis Detection Kit
KMAF-100T	ImmunoStep	100 test	EUR 504.6

MitoCapture Apoptosis Detection Kit
55R-1305	Fitzgerald	25 assays	EUR 332.4
Description: Apoptosis Kit for detection of apoptosis in the research laboratory

TUNEL FITC Apoptosis Detection Kit
A111-01	Vazyme	20 rxn	EUR 339.6

TUNEL FITC Apoptosis Detection Kit
A111-02	Vazyme	50 rxn	EUR 490.8

TUNEL FITC Apoptosis Detection Kit
A111-03	Vazyme	100 rxn	EUR 646.8

TUNEL BrightRed Apoptosis Detection Kit
A113-01	Vazyme	20 rxn	EUR 367.2

TUNEL BrightRed Apoptosis Detection Kit
A113-02	Vazyme	50 rxn	EUR 547.2

TUNEL BrightRed Apoptosis Detection Kit
A113-03	Vazyme	100 rxn	EUR 786

TUNEL BrightGreen Apoptosis Detection Kit
A112-01	Vazyme	20 rxn	EUR 367.2

TUNEL BrightGreen Apoptosis Detection Kit
A112-02	Vazyme	50 rxn	EUR 547.2

TUNEL BrightGreen Apoptosis Detection Kit
A112-03	Vazyme	100 rxn	EUR 786

Annexin V-PE Apoptosis Kit Plus
K203-100	Biovision	each	EUR 582

Annexin V-PE Apoptosis Kit Plus
K203-25	Biovision	each	EUR 280.8

Annexin V-PE Apoptosis Kit Plus
K203-400	Biovision	each	EUR 1136.4

Annexin V-PE Apoptosis Kit Plus
K2059-100	ApexBio	100 assays	EUR 619.2

Annexin V-PE Apoptosis Kit Plus
K2059-25	ApexBio	25 assays	EUR 301.2

Annexin V-PE Apoptosis Kit Plus
K2059-400	ApexBio	400 assays	EUR 1270.8

Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and CF594 Annexin V (50 assays)
30067	Biotium	1KIT	EUR 514.8
Description: Minimum order quantity: 1 unit of 1KIT

CaspSCREEN Flow Cytometric Apoptosis Detection Kit
55R-1301	Fitzgerald	25 assays	EUR 435.6
Description: Apoptosis Kit for use in the research laboratory

CaspSCREEN? Flow Cytometric Apoptosis Detection Kit
K200-100	Biovision	each	EUR 627.6

CaspSCREEN? Flow Cytometric Apoptosis Detection Kit
K200-25	Biovision	each	EUR 333.6

Aposcreen Annexin V Apoptosis Kit : PE (OKSB00008)
OKSB00008	Aviva Systems Biology	100 Tests	EUR 615.6
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: The ApoScreen Annexin V Apoptosis Kit employs a fluorescein- labeled annexin V (annexin V- FITC) in concert withpropidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis,cells begin to display PS on the outer cell membrane where it is readily detectable by staining the cells with annexin VFITC.As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readilymove across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lostmembrane integrity through mechanisms including necrosis. When cells are double stained with annexin V- FITC and PI,three different cell populations may be observed (Figure 2): (i) live cells that do not stain with either annexin V- FITC or PI;(ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with annexin V- FITC only. Analysis canbe performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells canbe examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.;Sensitivity:

Mitochondrial Apoptosis Detection Fluorometric Kit
K2097-100	ApexBio	100 assays	EUR 602.4

Mitochondrial Apoptosis Detection Fluorometric Kit
K2097-25	ApexBio	25 assays	EUR 285.6

Annexin V-Cy3 Apoptosis Kit
K102-100	Biovision	each	EUR 601.2

Annexin V-Cy3 Apoptosis Kit
K102-25	Biovision	each	EUR 248.4

Annexin V-Cy3 Apoptosis Kit
K102-400	Biovision	each	EUR 1149.6

Annexin V-Cy5 Apoptosis Kit
K103-100	Biovision	each	EUR 614.4

Annexin V-Cy5 Apoptosis Kit
K103-25	Biovision	each	EUR 261.6

Annexin V-Cy5 Apoptosis Kit
K103-400	Biovision	each	EUR 1227.6

Annexin V-Cy3 Apoptosis Kit
K2004-100	ApexBio	100 assays	EUR 619.2

Annexin V-Cy3 Apoptosis Kit
K2004-25	ApexBio	25 assays	EUR 285.6

Annexin V-Cy3 Apoptosis Kit
K2004-400	ApexBio	400 assays	EUR 1336.8

Annexin V-Cy5 Apoptosis Kit
K2005-100	ApexBio	100 assays	EUR 619.2

Annexin V-Cy5 Apoptosis Kit
K2005-25	ApexBio	25 assays	EUR 285.6

Annexin V-Cy5 Apoptosis Kit
K2005-400	ApexBio	400 assays	EUR 1336.8

In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit
20-abx098881	Abbexa	EUR 678.00 EUR 844.80	25 rxns 50 rxns

Annexin V-EGFP Apoptosis Kit
K104-100	Biovision	each	EUR 601.2

Annexin V-EGFP Apoptosis Kit
K104-25	Biovision	each	EUR 248.4

Annexin V-EGFP Apoptosis Kit
K104-400	Biovision	each	EUR 1149.6

Annexin V-FITC Apoptosis Kit
K101-100	Biovision	each	EUR 614.4

Annexin V-FITC Apoptosis Kit
K101-25	Biovision	each	EUR 268.8

Annexin V-FITC Apoptosis Kit
K101-400	Biovision	each	EUR 1227.6

Annexin V-FITC Apoptosis Kit
K2003-100	ApexBio	100 assays	EUR 619.2

Annexin V-FITC Apoptosis Kit
K2003-25	ApexBio	25 assays	EUR 285.6

Annexin V-FITC Apoptosis Kit
K2003-400	ApexBio	400 assays	EUR 1336.8

Annexin V-EGFP Apoptosis Kit
K2006-100	ApexBio	100 assays	EUR 619.2

Annexin V-EGFP Apoptosis Kit
K2006-25	ApexBio	25 assays	EUR 285.6

Annexin V-EGFP Apoptosis Kit
K2006-400	ApexBio	400 assays	EUR 1336.8

Annexin V-Biotin Apoptosis Kit
K109-100	Biovision	each	EUR 561.6

Annexin V-Biotin Apoptosis Kit
K109-25	Biovision	each	EUR 248.4

Annexin V-Biotin Apoptosis Kit
K109-400	Biovision	each	EUR 1149.6

Annexin V-Biotin Apoptosis Kit
K2009-100	ApexBio	100 assays	EUR 619.2

Annexin V-Biotin Apoptosis Kit
K2009-25	ApexBio	25 assays	EUR 285.6

Annexin V-Biotin Apoptosis Kit
K2009-400	ApexBio	400 assays	EUR 1336.8

Annexin V-Cy3 Apoptosis Kit Plus
K202-100	Biovision	each	EUR 588

Annexin V-Cy3 Apoptosis Kit Plus
K202-25	Biovision	each	EUR 288

Annexin V-Cy3 Apoptosis Kit Plus
K202-400	Biovision	each	EUR 1136.4

Annexin V-Cy3 Apoptosis Kit Plus
K2058-100	ApexBio	100 assays	EUR 619.2

Annexin V-Cy3 Apoptosis Kit Plus
K2058-25	ApexBio	25 assays	EUR 301.2

Annexin V-Cy3 Apoptosis Kit Plus
K2058-400	ApexBio	400 assays	EUR 1270.8

Annexin V-FITC Apoptosis Kit Plus
K201-100	Biovision	each	EUR 601.2

Annexin V-FITC Apoptosis Kit Plus
K201-25	Biovision	each	EUR 288

Annexin V-FITC Apoptosis Kit Plus
K201-400	Biovision	each	EUR 1136.4

Annexin V-FITC Apoptosis Kit Plus
K2057-100	ApexBio	100 assays	EUR 619.2

Annexin V-FITC Apoptosis Kit Plus
K2057-25	ApexBio	25 assays	EUR 301.2

Annexin V-FITC Apoptosis Kit Plus
K2057-400	ApexBio	400 assays	EUR 1270.8

Mitochondrial Apoptosis Detection Fluorometric Kit, MitoCapture
K250-100	Biovision	each	EUR 588

Mitochondrial Apoptosis Detection Fluorometric Kit, MitoCapture
K250-25	Biovision	each	EUR 274.8

Inhibitor of Apoptosis Protein Detection Set
PSI-1815	ProSci	1 Set	EUR 1041.9
Description: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. This process can be interrupted by a family of proteins termed inhibitors of apoptosis (IAP). Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR). These BIRs promote protein-protein interactions with members of the TRAF family of signaling molecules as well as caspases such as caspase-3, -7, and -9, and inhibiting the apoptotic activity of these proteins. Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Other IAPs include the X-linked protein XIAP/ILP-1, the related cIAP protein which exists in two distinct isoforms, Livin (also known as KIAP as it is highly expressed in kidney), Survivin/TIAP, ILP-2, and Bruce, an extremely large IAP with ubiquitin-conjugating enzyme activity. Upregulation of these proteins inhibit the normal apoptotic process and thus may be involved in human diseases such as cancer.;;For images please see PDF data sheet

Aposcreen Annexin V Apoptosis Kit : FITC (OKSB00007)
OKSB00007	Aviva Systems Biology	100 Tests	EUR 615.6
Description: Description of target: ;Species reactivity: ;Application: FC, ICC;Assay info: ;Sensitivity:

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22828-100Tests	AAT Bioquest	100 Tests	EUR 222
Description: Our Cell Meterâ„¢ assay kits are a set of tools for monitoring cell viability.

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*
22829-100Tests	AAT Bioquest	100 Tests	EUR 222
Description: Our Cell Meterâ„¢ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22828	AAT Bioquest	100 Tests	EUR 222

Apart from, Luciferase reporter gene experiment revealed that H3F3A was a selected goal gene for microRNA-198. Furthermore, qRT-PCR indicated that H3F3A and microRNA-198 had been negatively correlated in thyroid carcinoma tissues. As well as, in contrast with NC group, overexpression of H3F3A markedly enhanced the migration and proliferative capability of TCa cells. Lastly, restoration experiment revealed a mutual regulation between microRNA-198 and H3F3A, the 2 of which can collectively take part within the malignant development of TCa.