August 26, 2020
Evaluation of RealStar Reverse Transcription-Polymerase Chain Reaction Kits
Analysis of RealStar Reverse Transcription-Polymerase Chain Response Kits for Filovirus Detection within the Laboratory and Area.
Prognosis of Ebola virus (EBOV) illness (EVD) requires laboratory testing.
The RealStar Filovirus Display screen reverse transcription-polymerase chain response (RT-PCR) equipment and the derived RealStar Zaire Ebolavirus RT-PCR equipment had been validated utilizing in vitro transcripts, supernatant of contaminated cell cultures, and medical specimens from sufferers with EVD.
The Filovirus Display screen equipment detected EBOV, Sudan virus, Taï Forest virus, Bundibugyo virus, Reston virus, and Marburg virus and differentiated between the genera Ebolavirus and Marburgvirus The quantity of filovirus RNA that might be detected with a likelihood of 95% ranged from 11 to 67 RNA copies/response on a LightCycler 480 II.
The Zaire Ebolavirus equipment is predicated on the Filovirus Display screen equipment however was optimized for detection of EBOV. It has an improved signal-to-noise ratio at low EBOV RNA concentrations and is considerably extra delicate than the Filovirus equipment. Each kits present considerably decrease analytical sensitivity on a SmartCycler II.
Scientific analysis revealed that the SmartCycler II, in contrast with different real-time PCR platforms, decreases the medical sensitivity of the Filovirus Display screen equipment to diagnose EVD at an early stage.
The Filovirus Display screen equipment detects all human-pathogenic filoviruses with good analytical sensitivity if carried out on an acceptable real-time PCR platform. Excessive analytical sensitivity is vital for early prognosis of EVD.
human anti-human CD163 mAb |
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E4A09C49 | EnoGene | 50ug | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
Mouse Anti Human CD163: RPE |
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MBS225636-100Tests | MyBiosource | 100Tests | EUR 520 |
Mouse Anti Human CD163: RPE |
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MBS225636-5x100Tests | MyBiosource | 5x100Tests | EUR 2160 |
MOUSE ANTI HUMAN CD163: FITC |
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MBS225948-01mg | MyBiosource | 0.1mg | EUR 435 |
MOUSE ANTI HUMAN CD163: FITC |
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MBS225948-5x01mg | MyBiosource | 5x0.1mg | EUR 1785 |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-48Tests | GenAsia Biotech | 48 Tests | Ask for price |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-96Tests | GenAsia Biotech | 96 Tests | EUR 388 |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human CD163 antigen(CD163)ISA kit |
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QY-E05066 | Qayee Biotechnology | 96T | EUR 433.2 |
human CD163 antigen,CD163 LISA kit |
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201-12-0247 | Shanghai Sunred Biological Technology | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
human CD163 antigen,CD163 ELISA kit |
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YLA0098HU-48T | Shanghai YL Biotech | 48T | Ask for price |
human CD163 antigen,CD163 ELISA kit |
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YLA0098HU-96T | Shanghai YL Biotech | 96T | Ask for price |
Human CD163 antigen,CD163 ELISA KIT |
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E0246Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | EUR 4122 |
Human CD163 antigen,CD163 ELISA KIT |
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E0246Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | EUR 300 |
Human CD163 antigen,CD163 ELISA KIT |
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E0246Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | EUR 2061 |
Human CD163 antigen,CD163 ELISA KIT |
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E0246Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human CD163 Antigen (CD163) ELISA Kit |
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MBS9353075-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human CD163 Antigen (CD163) ELISA Kit |
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MBS9353075-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human CD163 Antigen (CD163) ELISA Kit |
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MBS9353075-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human CD163 Antigen (CD163) ELISA Kit |
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MBS9353075-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Human CD163 antigen, CD163 ELISA Kit |
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MBS165165-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human CD163 antigen, CD163 ELISA Kit |
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MBS165165-48StripWells | MyBiosource | 48-Strip-Wells | EUR 285 |
Human CD163 antigen, CD163 ELISA Kit |
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MBS165165-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human CD163 antigen, CD163 ELISA Kit |
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MBS165165-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Human CD163 Antigen, CD163 GENLISA ELISA |
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KBH0246 | Krishgen | 1 x 96 wells | EUR 286 |
Mouse Anti Human Cd163 Monoclonal Antibody |
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CABT-47162MH | Creative Diagnostics | 25 µg | EUR 446.4 |
Mouse Anti Human Cd163 Monoclonal Antibody |
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CABT-47163MH | Creative Diagnostics | 0.2 mg | EUR 1057.2 |
Mouse Anti Human CD163, APC Conjugated mAb |
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MBS9431893-100Tests | MyBiosource | 100Tests | EUR 520 |
Mouse Anti Human CD163, APC Conjugated mAb |
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MBS9431893-25Tests | MyBiosource | 25Tests | EUR 275 |
Mouse Anti Human CD163, APC Conjugated mAb |
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MBS9431893-5x100Tests | MyBiosource | 5x100Tests | EUR 2195 |
CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 2 |
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SC308167 | Origene Technologies GmbH | 10 µg | Ask for price |
CD163 (untagged)-Human CD163 molecule (CD163), transcript variant 1 |
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SC117495 | Origene Technologies GmbH | 10 µg | Ask for price |
PE anti-human CD163 |
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E16FHP163-025 | EnoGene | 25 tests | EUR 433.33 |
Description: Available in various conjugation types. |
PE anti-human CD163 |
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E16FHP163-100 | EnoGene | 100 tests | EUR 953.33 |
Description: Available in various conjugation types. |
Anti-Human CD163 PE |
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MBS696987-100Tests | MyBiosource | 100Tests | EUR 415 |
Anti-Human CD163 PE |
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MBS696987-25Tests | MyBiosource | 25Tests | EUR 225 |
Anti-Human CD163 PE |
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MBS696987-5x100Tests | MyBiosource | 5x100Tests | EUR 1820 |
Anti-Human CD163 Antibody |
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101-M291 | ReliaTech | 100 µg | EUR 399 |
Description: CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes. |
APC anti-human CD163 |
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E16FHA163-025 | EnoGene | 25 tests | EUR 520 |
Description: Available in various conjugation types. |
APC anti-human CD163 |
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E16FHA163-100 | EnoGene | 100 tests | EUR 1191.67 |
Description: Available in various conjugation types. |
Anti-Human CD163 APC |
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MBS696989-100Tests | MyBiosource | 100Tests | EUR 480 |
Anti-Human CD163 APC |
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MBS696989-25Tests | MyBiosource | 25Tests | EUR 245 |
Anti-Human CD163 APC |
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MBS696989-5x100Tests | MyBiosource | 5x100Tests | EUR 2115 |
APC anti-human CD163 |
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MBS9459445-100Tests | MyBiosource | 100Tests | EUR 655 |
APC anti-human CD163 |
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MBS9459445-25Tests | MyBiosource | 25Tests | EUR 355 |
APC anti-human CD163 |
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MBS9459445-5x100Tests | MyBiosource | 5x100Tests | EUR 2790 |
FITC anti-human CD163 |
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E16FHF163-025 | EnoGene | 25 tests | EUR 520 |
Description: Available in various conjugation types. |
FITC anti-human CD163 |
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E16FHF163-100 | EnoGene | 100 tests | EUR 1191.67 |
Description: Available in various conjugation types. |
Biotin anti-human CD163 |
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E16FHB163-025U | EnoGene | 25 μg | EUR 346.67 |
Description: Available in various conjugation types. |
Biotin anti-human CD163 |
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E16FHB163-100U | EnoGene | 100 μg | EUR 780 |
Description: Available in various conjugation types. |
Purified anti-human CD163 |
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E16FHU163-100U | EnoGene | 100 μg | EUR 433.33 |
Description: Available in various conjugation types. |
CD163 (GFP-tagged) - Human CD163 molecule (CD163), transcript variant 2 |
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RG212754 | Origene Technologies GmbH | 10 µg | Ask for price |
CD163 (GFP-tagged) - Human CD163 molecule (CD163), transcript variant 1 |
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RG208557 | Origene Technologies GmbH | 10 µg | Ask for price |
Rabbit Anti Human CD163 Monoclonal Clone ABCC-3 |
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IRBAHUCD163ABCC3C100UL | Innovative research | each | EUR 496 |
Description: Rabbit Anti Human CD163 Monoclonal Clone ABCC-3 |
Recombinant Human CD163/CD163 Protein, His Tag |
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E40KMH346 | EnoGene | 20ug | EUR 495 |
CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 1 |
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RC208557 | Origene Technologies GmbH | 10 µg | Ask for price |
CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 2 |
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RC212754 | Origene Technologies GmbH | 10 µg | Ask for price |
Human CD163 Protein |
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E40KMPH6242 | EnoGene | 20ug | EUR 495 |
Recombinant Human CD163 |
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MBS147285-0001mg | MyBiosource | 0.001mg | EUR 240 |
Recombinant Human CD163 |
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MBS147285-0005mg | MyBiosource | 0.005mg | EUR 310 |
Recombinant Human CD163 |
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MBS147285-005mg | MyBiosource | 0.05mg | EUR 1360 |
Recombinant Human CD163 |
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MBS147285-5x005mg | MyBiosource | 5x0.05mg | EUR 5800 |
CD163 siRNA (Human) |
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MBS8229683-15nmol | MyBiosource | 15nmol | EUR 405 |
CD163 siRNA (Human) |
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MBS8229683-30nmol | MyBiosource | 30nmol | EUR 565 |
CD163 siRNA (Human) |
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MBS8229683-5x30nmol | MyBiosource | 5x30nmol | EUR 2450 |
Anti-Human CD163 Antibody (M130) |
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MBS1564482-01mg | MyBiosource | 0.1mg | EUR 340 |
Anti-Human CD163 Antibody (M130) |
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MBS1564482-5x01mg | MyBiosource | 5x0.1mg | EUR 1235 |
Anti-Human CD163 Antibody (AB101) |
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MBS1570008-01mg | MyBiosource | 0.1mg | EUR 405 |
Anti-Human CD163 Antibody (AB101) |
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MBS1570008-5x01mg | MyBiosource | 5x0.1mg | EUR 1520 |
PE-Cy7 anti-human CD163 |
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E16FHN163-025 | EnoGene | 25 tests | EUR 650 |
Description: Available in various conjugation types. |
PE-Cy7 anti-human CD163 |
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E16FHN163-100 | EnoGene | 100 tests | EUR 1408.33 |
Description: Available in various conjugation types. |
APC-Cy7 anti-human CD163 |
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E16FHO163-025 | EnoGene | 25 tests | EUR 650 |
Description: Available in various conjugation types. |
APC-Cy7 anti-human CD163 |
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E16FHO163-100 | EnoGene | 100 tests | EUR 1408.33 |
Description: Available in various conjugation types. |
PerCP-Cy5.5 anti-human CD163 |
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E16FHS163-025 | EnoGene | 25 tests | EUR 541.67 |
Description: Available in various conjugation types. |
PerCP-Cy5.5 anti-human CD163 |
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E16FHS163-100 | EnoGene | 100 tests | EUR 1278.33 |
Description: Available in various conjugation types. |
Anti-Human CD163 PE-Cyanine7 |
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MBS696988-100Tests | MyBiosource | 100Tests | EUR 505 |
Anti-Human CD163 PE-Cyanine7 |
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MBS696988-25Tests | MyBiosource | 25Tests | EUR 270 |
Anti-Human CD163 PE-Cyanine7 |
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MBS696988-5x100Tests | MyBiosource | 5x100Tests | EUR 2220 |
Mouse Monoclonal anti-human CD163 |
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hAP-0161 | Angio Proteomie | 100ug | EUR 250 |
Mouse Monoclonal anti-human CD163 |
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hAP-0161A | Angio Proteomie | 50ug | EUR 400 |
Human, Rat, Mouse CD163 antibody |
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P0405 | Omnimabs | 100ul | EUR 150 |
Human CD163, Fc Tag |
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E24PHA282 | EnoGene | 50 μg | EUR 250 |
Human CD163, His Tag |
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E24PHA281 | EnoGene | 50 μg | EUR 250 |
Human CD163 ELISA Kit |
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EK5296 | SAB | 96 tests | EUR 599 |
Human CD163 ELISA Kit |
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ELI-05603h | Nova Lifetech | 96tests | EUR 696 |
Human CD163 ELISA Kit |
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ELA-E1726h | Nova Lifetech | 96tests | EUR 696 |
Human CD163 ELISA Kit |
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GWB-SKR207 | GenWay Biotech | 96 Tests | Ask for price |
Human CD163 ELISA Kit |
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LF-EK50890 | Abfrontier | 1×96T | EUR 777.6 |
Human CD163 ELISA Kit |
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MBS824474-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Human CD163 ELISA Kit |
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MBS824474-96Tests | MyBiosource | 96Tests | EUR 645 |
Human CD163 ELISA Kit |
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MBS3800092-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human CD163 ELISA Kit |
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MBS3800092-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Analysis of Ebola Virus Inhibitors for Drug Repurposing.
A systematic display of FDA-approved medicine was carried out to establish compounds with in vitro antiviral actions in opposition to Ebola virus (EBOV). Compounds lively (>50% viral inhibition and <30% mobile toxicity) at a single focus had been examined in dose-response assays to quantitate the antiviral actions in replication and viral entry assays in addition to cytotoxicity within the Vero cell line used to conduct these assays.
On the idea of the accepted human dosing, toxicity/tolerability, and pharmacokinetic knowledge, seven of those in vitro hits from completely different pharmacological lessons (chloroquine (CQ), amiodarone, prochlorperazine, benztropine, azithromycin, chlortetracycline, and clomiphene) had been evaluated for his or her in vivo efficacy at a single dose and had been administered by way of both intraperitoneal (ip) or oral route.
Initially, azithromycin (100 mg/kg, twice day by day, ip), CQ (90 mg/kg, twice day by day, ip), and amiodarone (60 mg/kg, twice day by day, ip) demonstrated vital will increase in survival within the mouse mannequin. After repeat analysis, solely CQ was discovered to reproducibly give vital efficacy within the mouse mannequin with this dosing routine.
Azithromycin and CQ had been additionally examined in a guinea pig mannequin of EBOV an infection over a variety of doses, however not one of the doses elevated survival, and drug-related toxicity was noticed at decrease doses than within the mouse. These outcomes present the advantages and particular challenges related to drug repurposing and spotlight the necessity for cautious analysis of accepted medicine as quickly deployable countermeasures in opposition to future pandemics.