August 26, 2020
Evaluation of RealStar Reverse Transcription-Polymerase Chain Reaction Kits
Analysis of RealStar Reverse Transcription-Polymerase Chain Response Kits for Filovirus Detection within the Laboratory and Area.
Prognosis of Ebola virus (EBOV) illness (EVD) requires laboratory testing.
The RealStar Filovirus Display screen reverse transcription-polymerase chain response (RT-PCR) equipment and the derived RealStar Zaire Ebolavirus RT-PCR equipment had been validated utilizing in vitro transcripts, supernatant of contaminated cell cultures, and medical specimens from sufferers with EVD.
The Filovirus Display screen equipment detected EBOV, Sudan virus, Taï Forest virus, Bundibugyo virus, Reston virus, and Marburg virus and differentiated between the genera Ebolavirus and Marburgvirus The quantity of filovirus RNA that might be detected with a likelihood of 95% ranged from 11 to 67 RNA copies/response on a LightCycler 480 II.
The Zaire Ebolavirus equipment is predicated on the Filovirus Display screen equipment however was optimized for detection of EBOV. It has an improved signal-to-noise ratio at low EBOV RNA concentrations and is considerably extra delicate than the Filovirus equipment. Each kits present considerably decrease analytical sensitivity on a SmartCycler II.
Scientific analysis revealed that the SmartCycler II, in contrast with different real-time PCR platforms, decreases the medical sensitivity of the Filovirus Display screen equipment to diagnose EVD at an early stage.
The Filovirus Display screen equipment detects all human-pathogenic filoviruses with good analytical sensitivity if carried out on an acceptable real-time PCR platform. Excessive analytical sensitivity is vital for early prognosis of EVD.

sebov-detection
Human Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 522 |
Human Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 724 |
Anti-CD163 |
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DB-045-0.1 | DB Biotech | 100 μl | EUR 212 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-0.2 | DB Biotech | 200 μl | EUR 298 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-0.5 | DB Biotech | 500 μl | EUR 384 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-1 | DB Biotech | 1 ml | EUR 613 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-RTU-15 | DB Biotech | 15 ml | EUR 354 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
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DB-045-RTU-7 | DB Biotech | 7 ml | EUR 231 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
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DB045RTU-15 | DB Biotech | 15 ml | EUR 354 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
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DB045RTU-7 | DB Biotech | 7 ml | EUR 231 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Mu-48T | DL Develop | 48T | EUR 508 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Mu-96T | DL Develop | 96T | EUR 661 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Ra-48T | DL Develop | 48T | EUR 508 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Ra-96T | DL Develop | 96T | EUR 661 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 742 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 742 |
Anti-CD163 Antibody |
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PA1874 | BosterBio | 100ug/vial | EUR 294 |
Anti-CD163 Antibody |
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PA1874-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-CD163 Antibody |
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A00812-1 | BosterBio | 100ug/vial | EUR 334 |
Anti-CD163 antibody |
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STJ110681 | St John's Laboratory | 100 µl | EUR 277 |
Description: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
Anti-CD163 antibody |
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STJ160082 | St John's Laboratory | 1 mL C | EUR 941 |
Anti-CD163 antibody |
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STJ16100302 | St John's Laboratory | 100 µg | EUR 720 |
Anti-CD163 antibody |
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STJ16100324 | St John's Laboratory | 1 mL | EUR 979 |
Anti-CD163 antibody |
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STJ180301 | St John's Laboratory | 0.1 ml | EUR 215 |
Anti-CD163 Antibody |
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STJ500423 | St John's Laboratory | 100 µg | EUR 476 |
Anti-CD163 Antibody |
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STJ500424 | St John's Laboratory | 100 µg | EUR 476 |
Anti-CD163 antibody |
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STJ190009 | St John's Laboratory | 200 µl | EUR 197 |
Description: Unconjugated Mouse monoclonal to CD163 (AS1A1) |
Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
Anti-Hu CD163 APC |
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1A-645-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD163 APC |
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1A-645-T100 | ExBio | 100 tests | EUR 240 |
Anti-Hu CD163 PE |
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1P-645-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD163 PE |
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1P-645-T100 | ExBio | 100 tests | EUR 240 |
Anti-CD163/M130 Antibody |
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A00812 | BosterBio | 100ul | EUR 397 |
Description: Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130 (CD163) detection.tested for IF, IHC, WB in Human, Mouse, Rat. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details. |
Anti-Hu CD163 Purified |
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11-645-C025 | ExBio | 0.025 mg | EUR 99 |
Anti-Hu CD163 Purified |
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11-645-C100 | ExBio | 0.1 mg | EUR 158 |
Anti-CD163 Antibody BIOTIN |
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STJ500425 | St John's Laboratory | 100 µg | EUR 586 |
Anti-CD163 Antibody FITC |
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STJ500426 | St John's Laboratory | 100 µg | EUR 586 |
Anti-CD163 Antibody BIOTIN |
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STJ500427 | St John's Laboratory | 100 µg | EUR 586 |
Anti-CD163 Antibody FITC |
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STJ500428 | St John's Laboratory | 100 µg | EUR 586 |
anti- CD163/M130 Antibody |
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FNab09771 | FN Test | 100µg | EUR 548.75 |
Description: Antibody raised against CD163/M130 |
Anti-CD163/M130 antibody |
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PAab09771 | Lifescience Market | 100 ug | EUR 355 |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-48T | GenAsia Biotech | 48T | EUR 289 |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-96T | GenAsia Biotech | 96T | EUR 466 |
human CD163 antigen,CD163 LISA kit |
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201-12-0247 | SunredBio | 96 tests | EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human CD163 antigen(CD163)ISA kit |
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QY-E05066 | Qayee Biotechnology | 96T | EUR 361 |
Mouse Anti Human Cd163 Monoclonal Antibody |
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CABT-47162MH | Creative Diagnostics | 25 µg | EUR 372 |
Mouse Anti Human Cd163 Monoclonal Antibody |
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CABT-47163MH | Creative Diagnostics | 0.2 mg | EUR 881 |
Anti-CD163 Rabbit Monoclonal Antibody |
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M00812 | BosterBio | 100ug/vial | EUR 397 |
Description: Rabbit Monoclonal CD163 Antibody. Validated in IP, WB and tested in Human. |
Anti-Hu CD163 PE-Cy7 |
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T7-645-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD163 PE-Cy7 |
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T7-645-T100 | ExBio | 100 tests | EUR 268 |
Anti-Hu CD163 PerCP-Cy5.5 |
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T9-645-T025 | ExBio | 25 tests | EUR 196 |
Anti-Hu CD163 PerCP-Cy5.5 |
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T9-645-T100 | ExBio | 100 tests | EUR 352 |
Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
Porcine CD163 antigen,CD163 LISA kit |
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GA-E0045PC-48T | GenAsia Biotech | 48T | EUR 364 |
Porcine CD163 antigen,CD163 LISA kit |
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GA-E0045PC-96T | GenAsia Biotech | 96T | EUR 590 |
Canine CD163 antigen(CD163 LISA kit |
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GA-E0062CN-48T | GenAsia Biotech | 48T | EUR 402 |
Canine CD163 antigen(CD163 LISA kit |
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GA-E0062CN-96T | GenAsia Biotech | 96T | EUR 684 |
Mouse Anti Rat Cd163 Monoclonal Antibody |
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DMABT-47170MR | Creative Diagnostics | 0.25 mg | EUR 741 |
Mouse Anti Rat Cd163 Monoclonal Antibody |
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DMABT-47171MR | Creative Diagnostics | 0.1 mg | EUR 559 |
CD163 Antibody |
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20-abx242956 | Abbexa |
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CD163 Antibody |
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37157-100ul | SAB | 100ul | EUR 252 |
CD163 siRNA |
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20-abx910930 | Abbexa |
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CD163 siRNA |
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20-abx910931 | Abbexa |
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Cd163 Antibody |
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1-CSB-PA650452LA01MO | Cusabio |
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Description: A polyclonal antibody against Cd163. Recognizes Cd163 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000 |
CD163 Antibody |
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1-CSB-PA969205 | Cusabio |
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Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100 |
CD163 Antibody |
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1-CSB-PA801238LA01HU | Cusabio |
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Description: A polyclonal antibody against CD163. Recognizes CD163 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200 |
CD163 Antibody |
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49553-100ul | SAB | 100ul | EUR 333 |
CD163 Antibody |
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49553-50ul | SAB | 50ul | EUR 239 |
CD163 Antibody |
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DF8235 | Affbiotech | 200ul | EUR 304 |
Description: CD163 Antibody detects endogenous levels of total CD163. |
CD163 Antibody |
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ABD8235 | Lifescience Market | 100 ug | EUR 438 |
CD163 antibody |
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10R-6496 | Fitzgerald | 100 ug | EUR 203 |
Description: Mouse monoclonal CD163 antibody |
CD163 antibody |
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10R-CD163aRT | Fitzgerald | 250 ug | EUR 716 |
Description: Mouse monoclonal CD163 antibody |
Mouse Anti-Human CD163 monoclonal antibody, clone JID274 |
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CABT-L2938-100uL500uL | Creative Diagnostics | 100 uL, 500 uL | EUR 502 |
Anti-Human CD163 DyLight® 488 conjugated Antibody |
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A00812-Dyl488 | BosterBio | 100ug/vial | EUR 344 |
Anti-Human CD163 DyLight® 550 conjugated Antibody |
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A00812-Dyl550 | BosterBio | 100ug/vial | EUR 344 |
Human CD163 shRNA Plasmid |
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20-abx956176 | Abbexa |
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Human CD163 ELISA Kit |
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LF-EK50890 | Abfrontier | 1×96T | EUR 648 |
Human CD163 ELISA Kit |
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ELA-E1726h | Lifescience Market | 96 Tests | EUR 824 |
Human CellExp? CD163, Human recombinant |
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9226-10 | Biovision | EUR 305 |
Human CellExp? CD163, Human recombinant |
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9226-50 | Biovision | EUR 773 |
Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 |
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M00812-1 | BosterBio | 100uL | EUR 385 |
Description: Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 tested in WB, IHC, reactive to Human |
Mouse Anti Rat Cd163 Monoclonal Antibody,Biotin |
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DMABT-47168MR | Creative Diagnostics | 0.1 mg | EUR 881 |
Mouse Anti Rat Cd163 Monoclonal Antibody,FITC |
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DMABT-47169MR | Creative Diagnostics | 0.1 mg | EUR 881 |
Mouse Anti Rat Cd163 Monoclonal Antibody,RPE |
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DMABT-47172MR | Creative Diagnostics | 100 TEST | EUR 892 |
Mouse Anti Pig Cd163 Monoclonal Antibody,FITC |
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CABT-47164MP | Creative Diagnostics | 0.1 mg | EUR 881 |
Analysis of Ebola Virus Inhibitors for Drug Repurposing.
A systematic display of FDA-approved medicine was carried out to establish compounds with in vitro antiviral actions in opposition to Ebola virus (EBOV). Compounds lively (>50% viral inhibition and <30% mobile toxicity) at a single focus had been examined in dose-response assays to quantitate the antiviral actions in replication and viral entry assays in addition to cytotoxicity within the Vero cell line used to conduct these assays.
On the idea of the accepted human dosing, toxicity/tolerability, and pharmacokinetic knowledge, seven of those in vitro hits from completely different pharmacological lessons (chloroquine (CQ), amiodarone, prochlorperazine, benztropine, azithromycin, chlortetracycline, and clomiphene) had been evaluated for his or her in vivo efficacy at a single dose and had been administered by way of both intraperitoneal (ip) or oral route.
Initially, azithromycin (100 mg/kg, twice day by day, ip), CQ (90 mg/kg, twice day by day, ip), and amiodarone (60 mg/kg, twice day by day, ip) demonstrated vital will increase in survival within the mouse mannequin. After repeat analysis, solely CQ was discovered to reproducibly give vital efficacy within the mouse mannequin with this dosing routine.
Azithromycin and CQ had been additionally examined in a guinea pig mannequin of EBOV an infection over a variety of doses, however not one of the doses elevated survival, and drug-related toxicity was noticed at decrease doses than within the mouse. These outcomes present the advantages and particular challenges related to drug repurposing and spotlight the necessity for cautious analysis of accepted medicine as quickly deployable countermeasures in opposition to future pandemics.