August 26, 2020
Evaluation of RealStar Reverse Transcription-Polymerase Chain Reaction Kits
Analysis of RealStar Reverse Transcription-Polymerase Chain Response Kits for Filovirus Detection within the Laboratory and Area.
Prognosis of Ebola virus (EBOV) illness (EVD) requires laboratory testing.
The RealStar Filovirus Display screen reverse transcription-polymerase chain response (RT-PCR) equipment and the derived RealStar Zaire Ebolavirus RT-PCR equipment had been validated utilizing in vitro transcripts, supernatant of contaminated cell cultures, and medical specimens from sufferers with EVD.
The Filovirus Display screen equipment detected EBOV, Sudan virus, Taï Forest virus, Bundibugyo virus, Reston virus, and Marburg virus and differentiated between the genera Ebolavirus and Marburgvirus The quantity of filovirus RNA that might be detected with a likelihood of 95% ranged from 11 to 67 RNA copies/response on a LightCycler 480 II.
The Zaire Ebolavirus equipment is predicated on the Filovirus Display screen equipment however was optimized for detection of EBOV. It has an improved signal-to-noise ratio at low EBOV RNA concentrations and is considerably extra delicate than the Filovirus equipment. Each kits present considerably decrease analytical sensitivity on a SmartCycler II.
Scientific analysis revealed that the SmartCycler II, in contrast with different real-time PCR platforms, decreases the medical sensitivity of the Filovirus Display screen equipment to diagnose EVD at an early stage.
The Filovirus Display screen equipment detects all human-pathogenic filoviruses with good analytical sensitivity if carried out on an acceptable real-time PCR platform. Excessive analytical sensitivity is vital for early prognosis of EVD.

sebov-detection
Human Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 626.4 |
Human Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 868.8 |
Anti-CD163 |
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DB-045-0.1 | DB Biotech | 100 μl | EUR 254.4 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-0.2 | DB Biotech | 200 μl | EUR 357.6 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-0.5 | DB Biotech | 500 μl | EUR 460.8 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-1 | DB Biotech | 1 ml | EUR 735.6 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
Anti-CD163 |
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DB-045-RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
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DB-045-RTU-7 | DB Biotech | 7 ml | EUR 277.2 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
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DB045RTU-15 | DB Biotech | 15 ml | EUR 424.8 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Anti-CD163 |
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DB045RTU-7 | DB Biotech | 7 ml | EUR 277.2 |
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Mu-48T | DL Develop | 48T | EUR 609.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Mu-96T | DL Develop | 96T | EUR 793.2 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation (CD163) in samples from serum, plasma or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Ra-48T | DL Develop | 48T | EUR 609.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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DLR-CD163-Ra-96T | DL Develop | 96T | EUR 793.2 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cluster Of Differentiation (CD163) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 613.2 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 850.8 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 613.2 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RD-CD163-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 850.8 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 640.8 |
Mouse Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 890.4 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 640.8 |
Rat Cluster Of Differentiation (CD163) ELISA Kit |
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RDR-CD163-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 890.4 |
Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
Anti-CD163 Antibody |
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A00812-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-CD163 Antibody |
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PA1874 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CD163 Antibody |
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PA1874-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-CD163 antibody |
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STJ110681 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
Anti-CD163 Antibody |
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STJ500423 | St John's Laboratory | 100 µg | EUR 571.2 |
Anti-CD163 Antibody |
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STJ500424 | St John's Laboratory | 100 µg | EUR 571.2 |
Anti-CD163 antibody |
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STJ160082 | St John's Laboratory | 1 mL C | EUR 1129.2 |
Anti-CD163 antibody |
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STJ16100302 | St John's Laboratory | 100 µg | EUR 864 |
Anti-CD163 antibody |
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STJ16100324 | St John's Laboratory | 1 mL | EUR 1174.8 |
Anti-CD163 antibody |
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STJ180301 | St John's Laboratory | 0.1 ml | EUR 258 |
Anti-CD163 antibody |
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STJ190009 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Mouse monoclonal to CD163 (AS1A1) |
Anti-Hu CD163 Purified |
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11-645-C025 | ExBio | 0.025 mg | EUR 118.8 |
Anti-Hu CD163 Purified |
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11-645-C100 | ExBio | 0.1 mg | EUR 189.6 |
Anti-CD163/M130 Antibody |
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A00812 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit IgG polyclonal antibody for Scavenger receptor cysteine-rich type 1 protein M130 (CD163) detection.tested for IF, IHC, WB in Human, Mouse, Rat. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details. |
Anti-Hu CD163 APC |
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1A-645-T025 | ExBio | 25 tests | EUR 168 |
Anti-Hu CD163 APC |
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1A-645-T100 | ExBio | 100 tests | EUR 288 |
Anti-Hu CD163 PE |
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1P-645-T025 | ExBio | 25 tests | EUR 168 |
Anti-Hu CD163 PE |
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1P-645-T100 | ExBio | 100 tests | EUR 288 |
Anti-CD163/M130 antibody |
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PAab09771 | Lifescience Market | 100 ug | EUR 426 |
anti- CD163/M130 Antibody |
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FNab09771 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against CD163/M130 |
Anti-CD163 Antibody BIOTIN |
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STJ500425 | St John's Laboratory | 100 µg | EUR 703.2 |
Anti-CD163 Antibody FITC |
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STJ500426 | St John's Laboratory | 100 µg | EUR 703.2 |
Anti-CD163 Antibody BIOTIN |
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STJ500427 | St John's Laboratory | 100 µg | EUR 703.2 |
Anti-CD163 Antibody FITC |
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STJ500428 | St John's Laboratory | 100 µg | EUR 703.2 |
human CD163 antigen,CD163 LISA kit |
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201-12-0247 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human CD163 antigen(CD163)ISA kit |
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GA-E0263HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human CD163 antigen(CD163)ISA kit |
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QY-E05066 | Qayee Biotechnology | 96T | EUR 433.2 |
human CD163 antigen,CD163 ELISA kit |
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YLA0098HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
human CD163 antigen,CD163 ELISA kit |
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YLA0098HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Mouse Anti Human Cd163 Monoclonal Antibody |
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CABT-47162MH | Creative Diagnostics | 25 µg | EUR 446.4 |
Mouse Anti Human Cd163 Monoclonal Antibody |
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CABT-47163MH | Creative Diagnostics | 0.2 mg | EUR 1057.2 |
Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
Anti-CD163 Rabbit Monoclonal Antibody |
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M00812 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal CD163 Antibody. Validated in IP, WB and tested in Human. |
Anti-Hu CD163 PE-Cy7 |
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T7-645-T025 | ExBio | 25 tests | EUR 184.8 |
Anti-Hu CD163 PE-Cy7 |
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T7-645-T100 | ExBio | 100 tests | EUR 321.6 |
Anti-Hu CD163 PerCP-Cy5.5 |
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T9-645-T025 | ExBio | 25 tests | EUR 235.2 |
Anti-Hu CD163 PerCP-Cy5.5 |
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T9-645-T100 | ExBio | 100 tests | EUR 422.4 |
Purified Anti-human CD163 Antibody *GHI/61* |
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11630000 | AAT Bioquest | 100 ug | EUR 365 |
APC Anti-human CD163 Antibody *GHI/61* |
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116301B0 | AAT Bioquest | 25 tests | EUR 383 |
APC Anti-human CD163 Antibody *GHI/61* |
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116301B1 | AAT Bioquest | 100 tests | EUR 995 |
APC Anti-human CD163 Antibody *GHI/61* |
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116301B2 | AAT Bioquest | 500 tests | EUR 2984 |
FITC Anti-human CD163 Antibody *GHI/61* |
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116301G0 | AAT Bioquest | 100 tests | EUR 547 |
FITC Anti-human CD163 Antibody *GHI/61* |
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116301G1 | AAT Bioquest | 500 tests | EUR 2051 |
TRITC Anti-human CD163 Antibody *GHI/61* |
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116301H0 | AAT Bioquest | 100 tests | EUR 547 |
TRITC Anti-human CD163 Antibody *GHI/61* |
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116301H1 | AAT Bioquest | 500 tests | EUR 2051 |
PacBlue Anti-human CD163 Antibody *GHI/61* |
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116301I0 | AAT Bioquest | 100 tests | EUR 547 |
PacBlue Anti-human CD163 Antibody *GHI/61* |
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116301I1 | AAT Bioquest | 500 tests | EUR 2051 |
PacOrange Anti-human CD163 Antibody *GHI/61* |
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116301J0 | AAT Bioquest | 100 tests | EUR 547 |
PacOrange Anti-human CD163 Antibody *GHI/61* |
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116301J1 | AAT Bioquest | 500 tests | EUR 2051 |
PE Anti-human CD163 Antibody *GHI/61* |
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116301K0 | AAT Bioquest | 25 tests | EUR 383 |
PE Anti-human CD163 Antibody *GHI/61* |
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116301K1 | AAT Bioquest | 100 tests | EUR 995 |
PE Anti-human CD163 Antibody *GHI/61* |
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116301K2 | AAT Bioquest | 500 tests | EUR 2984 |
PerCP Anti-human CD163 Antibody *GHI/61* |
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116301S0 | AAT Bioquest | 25 tests | EUR 383 |
PerCP Anti-human CD163 Antibody *GHI/61* |
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116301S1 | AAT Bioquest | 100 tests | EUR 995 |
PerCP Anti-human CD163 Antibody *GHI/61* |
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116301S2 | AAT Bioquest | 500 tests | EUR 2984 |
Porcine CD163 antigen,CD163 LISA kit |
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GA-E0045PC-48T | GenAsia Biotech | 48T | EUR 436.8 |
Porcine CD163 antigen,CD163 LISA kit |
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GA-E0045PC-96T | GenAsia Biotech | 96T | EUR 708 |
Canine CD163 antigen(CD163 LISA kit |
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GA-E0062CN-48T | GenAsia Biotech | 48T | EUR 482.4 |
Canine CD163 antigen(CD163 LISA kit |
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GA-E0062CN-96T | GenAsia Biotech | 96T | EUR 820.8 |
Porcine CD163 antigen,CD163 LISA kit |
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YLA0139PO-48T | Shanghai YL Biotech | 48T | EUR 502.5 |
Porcine CD163 antigen,CD163 LISA kit |
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YLA0139PO-96T | Shanghai YL Biotech | 96T | EUR 618.75 |
CD163 Antibody |
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49553-100ul | SAB | 100ul | EUR 399.6 |
CD163 Antibody |
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49553-50ul | SAB | 50ul | EUR 286.8 |
CD163 Antibody |
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37157-100ul | SAB | 100ul | EUR 302.4 |
CD163 antibody |
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10R-6496 | Fitzgerald | 100 ug | EUR 243.6 |
Description: Mouse monoclonal CD163 antibody |
CD163 antibody |
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10R-CD163aRT | Fitzgerald | 250 ug | EUR 859.2 |
Description: Mouse monoclonal CD163 antibody |
CD163 Antibody |
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ABD8235 | Lifescience Market | 100 ug | EUR 525.6 |
Analysis of Ebola Virus Inhibitors for Drug Repurposing.
A systematic display of FDA-approved medicine was carried out to establish compounds with in vitro antiviral actions in opposition to Ebola virus (EBOV). Compounds lively (>50% viral inhibition and <30% mobile toxicity) at a single focus had been examined in dose-response assays to quantitate the antiviral actions in replication and viral entry assays in addition to cytotoxicity within the Vero cell line used to conduct these assays.
On the idea of the accepted human dosing, toxicity/tolerability, and pharmacokinetic knowledge, seven of those in vitro hits from completely different pharmacological lessons (chloroquine (CQ), amiodarone, prochlorperazine, benztropine, azithromycin, chlortetracycline, and clomiphene) had been evaluated for his or her in vivo efficacy at a single dose and had been administered by way of both intraperitoneal (ip) or oral route.
Initially, azithromycin (100 mg/kg, twice day by day, ip), CQ (90 mg/kg, twice day by day, ip), and amiodarone (60 mg/kg, twice day by day, ip) demonstrated vital will increase in survival within the mouse mannequin. After repeat analysis, solely CQ was discovered to reproducibly give vital efficacy within the mouse mannequin with this dosing routine.
Azithromycin and CQ had been additionally examined in a guinea pig mannequin of EBOV an infection over a variety of doses, however not one of the doses elevated survival, and drug-related toxicity was noticed at decrease doses than within the mouse. These outcomes present the advantages and particular challenges related to drug repurposing and spotlight the necessity for cautious analysis of accepted medicine as quickly deployable countermeasures in opposition to future pandemics.