December 23, 2020
CCAT2 contributes to progression and treatment resistance of thyroid carcinoma
Goal: The intention of this research is to uncover the correlations of the expression of colon most cancers related transcript 2 (CCAT2) within the medical papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC) specimens with the prognosis and chemoresistance of sufferers.
Sufferers and strategies: The expression degree of CCAT2 within the PTC and ATC specimens was decided utilizing Actual-Time quantitative Polymerase Chain Response (RT-qPCR), and the correlations of CCAT2 expression with the medical options of sufferers had been detected through χ2 check. Apart from, survival evaluation was carried out to confirm the relation between CCAT2 expression and sufferers’ survival. After knockdown or overexpression of CCAT2, the adjustments within the proliferation skill of human thyroid carcinoma cells had been examined through Cell Counting package-8 (CCK-8) assay, and the half maximal inhibitory focus (IC50) values of doxorubicin and cisplatin had been measured by methyl thiazolyl tetrazolium (MTT) assay.
Outcomes: In line with the χ2-test outcomes, the expression of CCAT2 was notably correlated with the capsular invasion and lymph node metastasis of PTC, and the capsular invasion, tumor dimension, and lymph node metastasis of ATC. It was found via the survival evaluation that the expression of CCAT2 was notably related to the poor prognosis of ATC sufferers. After knockdown of CCAT2, each the proliferation skill and the IC50 values of doxorubicin and cisplatin considerably declined in human thyroid carcinoma cells. The other situations had been discovered after CCAT2 was overexpressed in human thyroid carcinoma cells.
Conclusions: CCAT2 potentiates the proliferation skill and chemoresistance of cells, promotes the development of thyroid carcinoma, and hinders the prognosis of ATC.
Isolation and Characterization of Coronavirus and Rotavirus Related to Calves in Central A part of Oromia, Ethiopia
Background: Coronavirus and rotavirus are mostly related etiologies for calves’ diarrhoea, leading to lack of productiveness and financial system of farmers. Nonetheless, varied sides of diarrheal illness attributable to coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross-sectional research was carried out with the intention of isolation and molecular characterization of coronavirus and rotavirus from calves within the central a part of Oromia (Bishoftu, Sebata, Holeta, and Addis Ababa), Ethiopia, from November 2018 to Could 2019. The 4 research areas had been purposively chosen and faecal samples had been collected by easy random sampling for analysis of coronavirus and rotavirus an infection by utilizing the antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) package. As well as, this research was carried out to have perception in prevalence and related danger components of coronavirus and rotavirus an infection in calves.
End result: Throughout the research, 83 diarrheic and 162 nondiarrheic faecal samples collected from calves lower than Four weeks of age had been screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 pattern (1.2%) was constructive for coronavirus antigen and 6 samples (7.2%) had been discovered to be constructive for rotavirus antigen by Ag-ELISA. All of the nondiarrheic samples had been unfavorable for each coronavirus and rotavirus Ag.
The general prevalence of coronavirus and rotavirus an infection in calves was estimated at 0.4% (1/245) and a couple of.45% (6/245), respectively. All samples (7) of ELISA check constructive of each coronavirus and rotavirus had been propagated in Madin-Darby bovine kidney (MDBK) cells. After three subsequent passages, progressive cytopathic impact (CPE), i.e., rounding, detachment, and the destruction of monolayer cell of 5 samples (1 pattern of coronavirus and Four samples of rotavirus) (71.4%) had been noticed. On the molecular stage, reverse transcriptase polymerase chain response (RT-PCR) method was used to find out the presence of coronavirus and rotavirus nucleic acid by utilizing particular primers. The 5 samples that had been coronavirus and rotavirus antigen constructive by ELISA and develop CPE on cell tradition had been additionally constructive on RT-PCR method. The prevalence of an infection peaked at 1st and 2nd weeks of age in male calves.
Conclusion: Diarrheal illness attributable to coronavirus and rotavirus has an incredible well being drawback in calves that interrupts manufacturing advantages with diminished weight acquire and elevated mortality and its potential for zoonotic unfold. So, the current findings present coronavirus and rotavirus an infection in calves in Ethiopia that must be addressed by practising early colostrum feeding in new child calves, utilizing vaccine, or bettering livestock administration.

CCAT2 contributes to progression and treatment resistance of thyroid carcinoma
MiR-204-5p promotes lipid synthesis in mammary epithelial cells by concentrating on SIRT1
Aims: Understanding the molecular mechanisms of lipid synthesis within the mammary gland is essential for regulating the extent and composition of lipids in milk. This research aimed to analyze the purposeful and molecular mechanisms of miR-204-5p in mammary epithelial cells to offer a theoretical foundation for milk lipid synthesis.
Strategies: Actual-time quantitative PCR was carried out to detect the transcriptional ranges of miR-204-5p and associated mRNA abundance in mammary epithelial cells. Western blotting was carried out to find out protein expression. Cell proliferation was assessed by Cell Counting Equipment-8. A dual-luciferase reporter assay was carried out to confirm the concentrating on relationship between miR-204-5p and SIRT1. siRNA and overexpression plasmids had been transfected into mouse HC11 mammary epithelial cells.
Acetamide, pure |
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GL1221-250 | Glentham Life Sciences | 250 | EUR 15.9 |
Acetamide, pure |
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GL1221-5 | Glentham Life Sciences | 5 | EUR 126.5 |
DNA Pure Kit |
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FYG601-100P | Yeastern Biotech | 100 Preps | Ask for price |
DNA Pure Kit |
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FYG601-300P | Yeastern Biotech | 300 Preps | Ask for price |
RNA Pure Kit |
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FYG602-100P | Yeastern Biotech | 100 Preps | Ask for price |
RNA Pure Kit |
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FYG602-50P | Yeastern Biotech | 50 Preps | Ask for price |
CHAPS, Ultra Pure |
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22024-100G | Biotium | 100G | EUR 700.8 |
Description: Minimum order quantity: 1 unit of 100G |
CHAPS, Ultra Pure |
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22024-10G | Biotium | 10G | EUR 163.2 |
Description: Minimum order quantity: 1 unit of 10G |
CHAPS, Ultra Pure |
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C0660-005 | GenDepot | 5g | EUR 198 |
CHAPS, Ultra Pure |
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C0660-010 | GenDepot | 10g | EUR 303.6 |
Curcumin, pure, 98% |
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GP7022-10MG | Glentham Life Sciences | 10 mg | EUR 123.6 |
Curcumin, pure, 98% |
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GP7022-250MG | Glentham Life Sciences | 250 mg | EUR 468 |
Curcumin, pure, 98% |
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GP7022-50MG | Glentham Life Sciences | 50 mg | EUR 192 |
Curcumin, pure, 98% |
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GP7022-10 | Glentham Life Sciences | 10 | EUR 72.2 |
Curcumin, pure, 98% |
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GP7022-250 | Glentham Life Sciences | 250 | EUR 356.8 |
Curcumin, pure, 98% |
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GP7022-50 | Glentham Life Sciences | 50 | EUR 129.2 |
CHAPSO, ultra Pure |
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C0661-001 | GenDepot | 1g | EUR 198 |
Agarose Ultra Pure |
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BA01101 | Bioatlas | 100g | EUR 147.6 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Agarose Ultra Pure |
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BA01102 | Bioatlas | 500g | EUR 399.6 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
SDS, 99% Ultra Pure |
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S0800-100 | GenDepot | 1Kg | EUR 375.6 |
SDS, 99% Ultra Pure |
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S0800-250 | GenDepot | 2.5 Kg | EUR 643.2 |
SDS, 99% Ultra Pure |
|||
S0800-500 | GenDepot | 5 Kg | EUR 1146 |
DTT, Ultra Pure, 15g |
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R7634-15g | ACTGene | each | EUR 228 |
DTT, Ultra Pure, 50g |
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R7634-50g | ACTGene | each | EUR 531.6 |
PAP protein (> 95% pure) |
|||
30C-CP1016 | Fitzgerald | 1 mg | EUR 360 |
Description: Purified native Human PAP protein (> 95% pure) |
PAP protein (> 40% pure) |
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30C-CP1016P | Fitzgerald | 1 mg | EUR 159.6 |
Description: Purified native Human PAP protein (> 40% pure) |
PSA protein (> 95% pure) |
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30C-CP1017 | Fitzgerald | 1 mg | EUR 548.4 |
Description: Purified native Human PSA protein (active enzyme) |
PSA protein (> 40% pure) |
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30C-CP1017P | Fitzgerald | 1 mg | EUR 289.2 |
Description: Purified native Human PSA protein |
PSA protein (> 98% pure) |
|||
30C-CP1017U | Fitzgerald | 1 mg | EUR 678 |
Description: Purified native Human PSA protein |
RBP protein (> 40% pure) |
|||
30C-CP1024P | Fitzgerald | 1 mg | EUR 100.8 |
Description: Purified native Human RBP protein (> 40% pure) |
TBG protein (> 95% pure) |
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30C-CP1025 | Fitzgerald | 1 mg | EUR 211.2 |
Description: Purified native Human TBG protein (> 95% pure) |
TBG protein (> 40% pure) |
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30C-CP1025P | Fitzgerald | 1 mg | EUR 164.4 |
Description: Purified native Human TBG protein (> 40% pure) |
TSH protein (>50% pure) |
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30C-CP1026P | Fitzgerald | 1 mg | EUR 1066.8 |
Description: Purified native Human TSH protein (>50% pure) |
PSA protein (> 95% pure) |
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30C-CP1099 | Fitzgerald | 100 ug | EUR 361.2 |
Description: Purified native Human PSA protein |
PSA protein (> 95% pure) |
|||
30C-CP1105 | Fitzgerald | 100 ug | EUR 516 |
Description: Purified native Human PSA protein |
PSA protein (> 95% pure) |
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30C-CP1118 | Fitzgerald | 100 ug | EUR 268.8 |
Description: Purified native Human PSA protein |
RBP protein (> 95% pure) |
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30R-AR022 | Fitzgerald | 100 ug | EUR 159.6 |
Description: Highly purified native Human RBP protein |
RBP protein (95% pure) |
|||
30R-AR022L | Fitzgerald | 1 mg | EUR 360 |
Description: Highly purified native Human RBP protein |
TSH protein (> 50% pure) |
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30R-AT009 | Fitzgerald | 1 mg | EUR 1066.8 |
Description: Purified native Human TSH protein (> 50% pure) |
CRP protein (>80% pure) |
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30-AC05PP | Fitzgerald | 5 mg | EUR 262.8 |
Description: Partially purified native Human CRP protein |
CRP protein (>95% pure) |
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30-AC05S | Fitzgerald | 5 mg | EUR 332.4 |
Description: Purified native Human CRP protein |
PSA protein (> 75% pure) |
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30-AP16S | Fitzgerald | 100 ug | EUR 181.2 |
Description: Purified native Human PSA protein |
TBG protein (> 98% pure) |
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30-AT30 | Fitzgerald | 1 mg | EUR 480 |
Description: Purified native Human TBG protein |
TBG protein (> 98% pure) |
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30-AT30S | Fitzgerald | 1 mg | EUR 363.6 |
Description: Purified native Human TBG protein |
TBG protein (> 80% pure) |
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30-AT35 | Fitzgerald | 1 mg | EUR 289.2 |
Description: Purified native Human TBG protein |
PAP protein (> 30% pure) |
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30-1026 | Fitzgerald | 100 units | EUR 188.4 |
Description: Purified native Human PAP protein |
PAP protein (> 98% pure) |
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30-1027 | Fitzgerald | 1 kU | EUR 510 |
Description: Purified native Human PAP protein |
PSA protein (> 98% pure) |
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30-1205 | Fitzgerald | 100 ug | EUR 316.8 |
Description: Purified native Human Prostate Specific Antigen protein |
TBG protein (> 70% pure) |
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30-1213 | Fitzgerald | 1 mg | EUR 326.4 |
Description: Purified native Human TBG protein |
TBG protein (> 98% pure) |
|||
30-1214 | Fitzgerald | 1 mg | EUR 823.2 |
Description: Purified native Human TBG protein |
EDTA, Ultra Pure, 1kg |
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R9410-1kg | ACTGene | each | EUR 228 |
Stearic acid, pure, 98% |
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GK7312-100G | Glentham Life Sciences | 100 g | EUR 160.8 |
Stearic acid, pure, 98% |
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GK7312-10G | Glentham Life Sciences | 10 g | EUR 62.4 |
Stearic acid, pure, 98% |
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GK7312-25G | Glentham Life Sciences | 25 g | EUR 79.2 |
Stearic acid, pure, 98% |
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GK7312-50G | Glentham Life Sciences | 50 g | EUR 103.2 |
Tris Base, Ultra Pure |
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T9200-100 | GenDepot | 1kg | EUR 200.4 |
Tris Base , Ultra Pure |
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T9200-500 | GenDepot | 5kg | EUR 619.2 |
Stearic acid, pure, 98% |
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GK7312-10 | Glentham Life Sciences | 10 | EUR 22.1 |
Stearic acid, pure, 98% |
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GK7312-100 | Glentham Life Sciences | 100 | EUR 102.7 |
Stearic acid, pure, 98% |
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GK7312-25 | Glentham Life Sciences | 25 | EUR 35.5 |
Stearic acid, pure, 98% |
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GK7312-50 | Glentham Life Sciences | 50 | EUR 55.4 |
Tris - Ultra Pure Grade |
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TB0194 | Bio Basic | 500g | EUR 89.23 |
Geneticin (G418, (>98% pure)) |
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ABT-800-01 | Alpha Diagnostics | 1 g | EUR 196.8 |
Geneticin (G418, (>98% pure)) |
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ABT-800-05 | Alpha Diagnostics | 5 g | EUR 489.6 |
Aprotinin, bovine (pure >98%) |
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RP-1532 | Alpha Diagnostics | 100 mg | EUR 343.2 |
Thioflavin T, Ultra Pure |
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T1002-001 | GenDepot | 1g | EUR 343.2 |
Human Growth Hormone (pure) |
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30R-AG020 | Fitzgerald | 1 mg | EUR 663.6 |
Description: Purified recombinant Human Human Growth Hormone (pure) |
Prolactin protein > 95% pure |
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30-1199 | Fitzgerald | 100 ug | EUR 2755.2 |
Description: Purified native Human Prolactin protein |
Prolactin protein > 50% pure |
|||
30-1200 | Fitzgerald | 100 ug | EUR 1182 |
Description: Purified native Human Prolactin protein |
Pure Plasmid Miniprep Kit |
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W0500-200 | 101Bio | - | Ask for price |
Pure Plasmid Miniprep Kit |
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W0500-50 | 101Bio | - | Ask for price |
Pure Plasmid Maxiprep Kit |
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W0503-10 | 101Bio | - | Ask for price |
Thiostatin Rat protein, pure |
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TSTN11-N-25 | Alpha Diagnostics | 25 ug | EUR 416.4 |
Fluoroorotic Acid, Ultra Pure |
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A2531-10000 | ApexBio | 10 g | EUR 510 |
Description: useful in the selection of orotodine-5'-phosphate decarboxylase mutants of S. cerevisiaestore at -20oC |
Fluoroorotic Acid, Ultra Pure |
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A2531-5000 | ApexBio | 5 g | EUR 309.6 |
Description: useful in the selection of orotodine-5'-phosphate decarboxylase mutants of S. cerevisiaestore at -20oC |
Locust bean gum, pure, 2400 - 2800 cps |
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GC0918-1KG | Glentham Life Sciences | 1 kg | EUR 160.8 |
Locust bean gum, pure, 2400 - 2800 cps |
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GC0918-1 | Glentham Life Sciences | 1 | EUR 102.7 |
High Pure dNTPs (2.5 mM) |
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20-abx098043 | Abbexa |
|
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High Pure dNTPs (10 mM) |
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20-abx098044 | Abbexa |
|
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Urea, Ultra Pure Grade, 99.5% |
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U0568-100 | GenDepot | 1kg | EUR 193.2 |
Rat Amylin, 8-37 aa, pure |
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AMYL18-P5 | Alpha Diagnostics | 500 ug | EUR 489.6 |
D-Dimer protein (> 98% pure) |
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30-1021 | Fitzgerald | 100 ug | EUR 807.6 |
Description: Purified native Human D-Dimer protein |
hCG protein (Intact) (> 98% pure) |
|||
30-AC83 | Fitzgerald | 100 ug | EUR 214.8 |
Description: Purified native Human hCG protein (Intact) (> 98% pure) |
Feline Cat IgG-Ultra Pure |
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31-1006 | Fitzgerald | 100 ug | EUR 100.8 |
Desderman pure Liquid 500 mL |
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CLE3234 | Scientific Laboratory Supplies | PK20 | EUR 338.15 |
Desderman pure Gel 1 litre |
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CLE3244 | Scientific Laboratory Supplies | PK10 | EUR 235.01 |
Human Growth Hormone (> 95% pure) |
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30C-CP1042 | Fitzgerald | 1 mg | EUR 742.8 |
Description: Purified native Human Human Growth Hormone (> 95% pure) |
Human Growth Hormone (> 40% pure) |
|||
30C-CP1042P | Fitzgerald | 1 mg | EUR 289.2 |
Description: Purified native Human Human Growth Hormone (> 40% pure) |
Human Growth Hormone (> 98% pure) |
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30-AH05 | Fitzgerald | 100 ug | EUR 360 |
Description: Purified native Human Human Growth Hormone (> 98% pure) |
Human Growth Hormone (> 98% pure) |
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30-AH05S | Fitzgerald | 100 ug | EUR 468 |
Description: Purified native Human Human Growth Hormone (> 98% pure) |
Human Growth Hormone (> 60% pure) |
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30-AH06 | Fitzgerald | 100 ug | EUR 158.4 |
Description: Purified native Human Human Growth Hormone (> 60% pure) |
Pure Plasmid 96 Miniprep Kit |
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W0506-496 | 101Bio | - | Ask for price |
Sheep Anti-Chicken IgY, pure |
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60319-SAF-BLK | Alpha Diagnostics | 1g; bulk | Ask for price |
4 hydroxynonenal (HNE), >98% pure |
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HNE51-1 | Alpha Diagnostics | 1 mg | EUR 242.4 |
4 hydroxynonenal (HNE), >98% pure |
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HNE51-5 | Alpha Diagnostics | 5 mg | EUR 781.2 |
Baceterial Plasmid dsDNA, >95% pure |
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PDNA15-N-100 | Alpha Diagnostics | 100 ug | EUR 489.6 |
Mouse IgG (H + L) Ultra Pure |
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31R-1041 | Fitzgerald | 1 mg | EUR 165.6 |
Description: Highly purified Mouse IgG (H + L) for use as a control or blocking reagent |
DL-Dithiothreitol/DTT - pure grade |
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ADC-P-083 | Creative Biolabs | unit | Ask for price |
Arium Extended Connection Set Pure |
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WAT4348 | Scientific Laboratory Supplies | EACH | EUR 184.8 |
CA 15-3 High Pure Protein |
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abx061025-10kU | Abbexa | 10 kU | EUR 2314.8 |
Victoria Pure Blue BO (C.I. 42595) |
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GT0164-5 | Glentham Life Sciences | 5 | EUR 55.4 |
Human Angiotensin I, pure (bioactive) |
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AT55-P-5 | Alpha Diagnostics | 5 mg | EUR 634.8 |
Human Angiotensin II, pure (bioactive) |
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AT65-P-5 | Alpha Diagnostics | 5 mg | EUR 343.2 |
ROX NHS ester, pure 6- isomer |
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A8106-25 | ApexBio | 25 mg | EUR 630 |
Description: ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel. |
ROX NHS ester, pure 6- isomer |
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A8106-5 | ApexBio | 5 mg | EUR 351.6 |
Description: ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel. |
Human Angiotensin III, pure (bioactive) |
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AT75-P-5 | Alpha Diagnostics | 5 mg | EUR 343.2 |
InLab Pure Pro-ISM (with ISM) |
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PHE0468 | Scientific Laboratory Supplies | EACH | EUR 577.2 |
Beta 2 Microglobulin (B2M) Partially Pure (40-90%) |
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B2M17-N-1 | Alpha Diagnostics | 1 mg | EUR 343.2 |
CRP protein (>95% pure) (Azide free) |
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30-AC05AF | Fitzgerald | 5 mg | EUR 332.4 |
Description: Purified native Human C-reactive protein with no preservative added |
Goat Anti-Mouse IgG, aff pure |
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40319 | Alpha Diagnostics | 1 mg | EUR 242.4 |
Mouse alpha Macroglobulin protein, pure |
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AMGL15-N-25 | Alpha Diagnostics | 25 ug | EUR 270 |
Hydrochloric Acid 25% v/v pure |
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H25VVJ | Scientific Laboratory Supplies | 2.5L | EUR 106.8 |
Apex Low Melt Agarose Ultra Pure |
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20-103 | Genesee Scientific | 25g/Unit | EUR 375 |
Apex Low Melt Agarose Ultra Pure |
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20-104 | Genesee Scientific | 100g/Unit | EUR 687 |
High Pure Viral Nucleic Acid Kit |
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5114403001 | Scientific Laboratory Supplies | EACH | EUR 353.82 |
Cell wall, sermi pure (B. Anthracis) |
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CWBA-5 | Alpha Diagnostics | 5 mg | EUR 634.8 |
Apex General Purpose Agarose Ultra Pure |
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20-102GP | Genesee Scientific | 500g/Unit | EUR 579 |
Goat Anti-Chlamydia IgG, aff pure |
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CLM13-A | Alpha Diagnostics | 0.5 mg | EUR 489.6 |
Lactoferrin, Apo, Human milk (>98% pure) |
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LTF16-N-100 | Alpha Diagnostics | 100 ug | EUR 270 |
Rabbit Anti-Ketamin IgG aff pure |
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KTM12-A | Alpha Diagnostics | 100ug | EUR 489.6 |
Rabbit Anti-Glycine IgG, aff pure |
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GLYC52-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Aspartate IgG, aff pure |
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ASP51-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-mPEG IgG, Affinity Pure |
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PEG11-A | Alpha Diagnostics | 100 ug | EUR 562.8 |
Rabbit Anti-Oxycodone IgG aff pure |
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OXYC12-A | Alpha Diagnostics | 1 mg | EUR 489.6 |
Rabbit Anti-Glutamate IgG, aff pure |
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GLM23-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Glutamine IgG, aff pure |
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GLN55-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Histamine IgG, aff pure |
|||
HIST12-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
beta 2 Microglobulin protein (> 95% pure) |
|||
30C-CP1022 | Fitzgerald | 1 mg | EUR 220.8 |
Description: Purified native Human beta 2 Microglobulin protein |
beta 2 Microglobulin protein (> 98% pure) |
|||
30C-CP1022U | Fitzgerald | 1 mg | EUR 283.2 |
Description: Purified native Human beta 2 Microglobulin protein |
beta 2 Microglobulin protein (> 95% pure) |
|||
30R-AM001 | Fitzgerald | 10 mg | EUR 807.6 |
Description: Purified native Human beta 2 Microglobulin protein |
4-hydroxyperoxy 2-nonenal, >98% pure |
|||
HNE52-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
Rabbit Anti-Salmonella IgG, aff pure |
|||
SLM11-A | Alpha Diagnostics | 0.5 ml | EUR 343.2 |
Troponin-I Pig Cardiac, pure protein |
|||
TNIC28-N-25 | Alpha Diagnostics | 25 ug | EUR 270 |
Troponin-I Rat Cardiac, pure protein |
|||
TNIC32-N-25 | Alpha Diagnostics | 25 ug | EUR 315.6 |
Troponin-I, Dog Cardiac, pure protein |
|||
TNIC33-N-25 | Alpha Diagnostics | 25 ug | EUR 270 |
Goat Anti-Rat C3 IgG, aff pure |
|||
C312-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Goat Anti-Dog C3 IgG, aff pure |
|||
C313-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Saponin (Quillaja bark) pure, (sapogenin ?10%) |
|||
AV-4031-10 | Alpha Diagnostics | 10 g | EUR 270 |
Troponin-C, Human Cardiac, pure protein |
|||
TNCC75-N-25 | Alpha Diagnostics | 25 ug | EUR 270 |
Troponin-I Mouse Cardiac, pure protein |
|||
TNIC27-N-25 | Alpha Diagnostics | 25 ug | EUR 315.6 |
Troponin-T, Human Cardiac, pure protein |
|||
TNTC65-N-25 | Alpha Diagnostics | 25 ug | EUR 270 |
Arium Smart Station Pure Water - Benchtop |
|||
WAT4344 | Scientific Laboratory Supplies | EACH | EUR 2035.2 |
Goat Anti-Mouse C3 IgG, aff pure |
|||
C311-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rat/Canine Angiotensin I, pure (bioactive) |
|||
AT56-P-1 | Alpha Diagnostics | 1 mg | EUR 343.2 |
Sheep Anti-Benzodiazepine IgG aff pure |
|||
BNZE13-A | Alpha Diagnostics | 1 mg | EUR 343.2 |
Rabbit Anti-Dinitrophenyl (DNP) aff pure |
|||
DNP13-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Rabbit Anti-Mouse Haptoglobin aff pure |
|||
HGLB11-A | Alpha Diagnostics | 100 ug | EUR 578.4 |
Outcomes: The abundance of miR-204-5p was a lot increased in lactating mouse mammary glands than in different tissues, which indicated that miR-204-5p could also be concerned in regulating milk manufacturing. MiR-204-5p affected the expression of β-casein and milk lipid synthesis in HC11 mouse mammary epithelial cells however didn’t affect the proliferation of HC11 cells. Overexpression of miR-204-5p significantly elevated the variety of Oil Pink O+ cells, triglyceride accumulation and the expression of markers related to lipid synthesis, together with FASN and PPARγ, whereas inhibition of miR-204-5p had the other impact. miR-204-5p promotes lipid synthesis by negatively regulating SIRT1. Overexpression of SIRT1 can repress the promotion of miR-204-5p on lipid synthesis.