December 23, 2020
CCAT2 contributes to progression and treatment resistance of thyroid carcinoma
Goal: The intention of this research is to uncover the correlations of the expression of colon most cancers related transcript 2 (CCAT2) within the medical papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC) specimens with the prognosis and chemoresistance of sufferers.
Sufferers and strategies: The expression degree of CCAT2 within the PTC and ATC specimens was decided utilizing Actual-Time quantitative Polymerase Chain Response (RT-qPCR), and the correlations of CCAT2 expression with the medical options of sufferers had been detected through χ2 check. Apart from, survival evaluation was carried out to confirm the relation between CCAT2 expression and sufferers’ survival. After knockdown or overexpression of CCAT2, the adjustments within the proliferation skill of human thyroid carcinoma cells had been examined through Cell Counting package-8 (CCK-8) assay, and the half maximal inhibitory focus (IC50) values of doxorubicin and cisplatin had been measured by methyl thiazolyl tetrazolium (MTT) assay.
Outcomes: In line with the χ2-test outcomes, the expression of CCAT2 was notably correlated with the capsular invasion and lymph node metastasis of PTC, and the capsular invasion, tumor dimension, and lymph node metastasis of ATC. It was found via the survival evaluation that the expression of CCAT2 was notably related to the poor prognosis of ATC sufferers. After knockdown of CCAT2, each the proliferation skill and the IC50 values of doxorubicin and cisplatin considerably declined in human thyroid carcinoma cells. The other situations had been discovered after CCAT2 was overexpressed in human thyroid carcinoma cells.
Conclusions: CCAT2 potentiates the proliferation skill and chemoresistance of cells, promotes the development of thyroid carcinoma, and hinders the prognosis of ATC.
Isolation and Characterization of Coronavirus and Rotavirus Related to Calves in Central A part of Oromia, Ethiopia
Background: Coronavirus and rotavirus are mostly related etiologies for calves’ diarrhoea, leading to lack of productiveness and financial system of farmers. Nonetheless, varied sides of diarrheal illness attributable to coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross-sectional research was carried out with the intention of isolation and molecular characterization of coronavirus and rotavirus from calves within the central a part of Oromia (Bishoftu, Sebata, Holeta, and Addis Ababa), Ethiopia, from November 2018 to Could 2019. The 4 research areas had been purposively chosen and faecal samples had been collected by easy random sampling for analysis of coronavirus and rotavirus an infection by utilizing the antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) package. As well as, this research was carried out to have perception in prevalence and related danger components of coronavirus and rotavirus an infection in calves.
End result: Throughout the research, 83 diarrheic and 162 nondiarrheic faecal samples collected from calves lower than Four weeks of age had been screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 pattern (1.2%) was constructive for coronavirus antigen and 6 samples (7.2%) had been discovered to be constructive for rotavirus antigen by Ag-ELISA. All of the nondiarrheic samples had been unfavorable for each coronavirus and rotavirus Ag.
The general prevalence of coronavirus and rotavirus an infection in calves was estimated at 0.4% (1/245) and a couple of.45% (6/245), respectively. All samples (7) of ELISA check constructive of each coronavirus and rotavirus had been propagated in Madin-Darby bovine kidney (MDBK) cells. After three subsequent passages, progressive cytopathic impact (CPE), i.e., rounding, detachment, and the destruction of monolayer cell of 5 samples (1 pattern of coronavirus and Four samples of rotavirus) (71.4%) had been noticed. On the molecular stage, reverse transcriptase polymerase chain response (RT-PCR) method was used to find out the presence of coronavirus and rotavirus nucleic acid by utilizing particular primers. The 5 samples that had been coronavirus and rotavirus antigen constructive by ELISA and develop CPE on cell tradition had been additionally constructive on RT-PCR method. The prevalence of an infection peaked at 1st and 2nd weeks of age in male calves.
Conclusion: Diarrheal illness attributable to coronavirus and rotavirus has an incredible well being drawback in calves that interrupts manufacturing advantages with diminished weight acquire and elevated mortality and its potential for zoonotic unfold. So, the current findings present coronavirus and rotavirus an infection in calves in Ethiopia that must be addressed by practising early colostrum feeding in new child calves, utilizing vaccine, or bettering livestock administration.

CCAT2 contributes to progression and treatment resistance of thyroid carcinoma
MiR-204-5p promotes lipid synthesis in mammary epithelial cells by concentrating on SIRT1
Aims: Understanding the molecular mechanisms of lipid synthesis within the mammary gland is essential for regulating the extent and composition of lipids in milk. This research aimed to analyze the purposeful and molecular mechanisms of miR-204-5p in mammary epithelial cells to offer a theoretical foundation for milk lipid synthesis.
Strategies: Actual-time quantitative PCR was carried out to detect the transcriptional ranges of miR-204-5p and associated mRNA abundance in mammary epithelial cells. Western blotting was carried out to find out protein expression. Cell proliferation was assessed by Cell Counting Equipment-8. A dual-luciferase reporter assay was carried out to confirm the concentrating on relationship between miR-204-5p and SIRT1. siRNA and overexpression plasmids had been transfected into mouse HC11 mammary epithelial cells.
Oxyma Pure |
|||
abx095068-200l | Abbexa | 200 µl | EUR 525 |
HiMaG Pure |
|||
PGE0015-1KG | EWC Diagnostics | 1 unit | EUR 5.15 |
Description: HiMaG Pure |
HiMaG Pure |
|||
PGE0015-500G | EWC Diagnostics | 1 unit | EUR 3.07 |
Description: HiMaG Pure |
HiCaN Pure |
|||
PGE0016-1KG | EWC Diagnostics | 1 unit | EUR 6.19 |
Description: HiCaN Pure |
HiCaN Pure |
|||
PGE0016-500G | EWC Diagnostics | 1 unit | EUR 3.18 |
Description: HiCaN Pure |
HiPoT Pure |
|||
PGE0017-1KG | EWC Diagnostics | 1 unit | EUR 12.37 |
Description: HiPoT Pure |
HiPoT Pure |
|||
PGE0017-500G | EWC Diagnostics | 1 unit | EUR 8.1 |
Description: HiPoT Pure |
HiBoR Pure |
|||
PGE0018-500G | EWC Diagnostics | 1 unit | EUR 7.88 |
Description: HiBoR Pure |
Pectin pure |
|||
45057 | Sisco Laboratories | 100 Gms | EUR 5.61 |
Description: Part A |
Sucrose pure |
|||
84973 | Sisco Laboratories | 500 Gms | EUR 1.98 |
Description: Part A |
purE Antibody |
|||
CSB-PA360356XA01ENV-02mg | Cusabio | 0.2mg | Ask for price |
Description: Recombinant Escherichia coli (strain K12) purE protein |
purE Antibody |
|||
CSB-PA360356XA01ENV-10mg | Cusabio | 10mg | Ask for price |
Description: Recombinant Escherichia coli (strain K12) purE protein |
purE Antibody |
|||
CSB-PA365392XA01EOD-02mg | Cusabio | 0.2mg | Ask for price |
Description: Recombinant Escherichia coli O157:H7 purE protein |
purE Antibody |
|||
CSB-PA365392XA01EOD-10mg | Cusabio | 10mg | Ask for price |
Description: Recombinant Escherichia coli O157:H7 purE protein |
Aluminon, Pure |
|||
RM283-100G | EWC Diagnostics | 1 unit | EUR 141.11 |
Description: Aluminon, Pure |
Aluminon, Pure |
|||
RM283-25G | EWC Diagnostics | 1 unit | EUR 40.36 |
Description: Aluminon, Pure |
Bentonite pure |
|||
92118 | Sisco Laboratories | 500 Gms | EUR 1.51 |
Description: Part A |
Acetamide, pure |
|||
GL1221 | Glentham Life Sciences | 250g | EUR 115.8 |
Acetamide, pure |
|||
GL1221-1 | Glentham Life Sciences | 1 | EUR 39.4 |
Acetamide, pure |
|||
GL1221-1KG | Glentham Life Sciences | 1 kg | EUR 84 |
Acetamide, pure |
|||
GL1221-250 | Glentham Life Sciences | 250 | EUR 15.9 |
Acetamide, pure |
|||
GL1221-250G | Glentham Life Sciences | 250 g | EUR 55.2 |
Acetamide, pure |
|||
GL1221-5 | Glentham Life Sciences | 5 | EUR 126.5 |
Acetamide, pure |
|||
GL1221-5KG | Glentham Life Sciences | 5 kg | EUR 189.6 |
Plasminogen Pure |
|||
GWB-248041 | GenWay Biotech | 1 mg | Ask for price |
FSH 98% Pure |
|||
GWB-DA26DC | GenWay Biotech | 0.1 mg | Ask for price |
hCG 98% pure |
|||
GWB-BEBACF | GenWay Biotech | 0.1 mg | Ask for price |
PSA 98% Pure |
|||
GWB-BFDD4C | GenWay Biotech | 1 mg | Ask for price |
hCG >98% pure |
|||
MBS318122-1mg | MyBiosource | 1mg | EUR 1585 |
hCG >98% pure |
|||
MBS318122-5x1mg | MyBiosource | 5x1mg | EUR 6960 |
PSA >98% Pure |
|||
MBS318336-1mg | MyBiosource | 1mg | EUR 2015 |
PSA >98% Pure |
|||
MBS318336-5x1mg | MyBiosource | 5x1mg | EUR 8905 |
FSH 98% Pure |
|||
MBS318382-01mg | MyBiosource | 0.1mg | EUR 3400 |
FSH 98% Pure |
|||
MBS318382-5x01mg | MyBiosource | 5x0.1mg | EUR 15130 |
hCG >=98% Pure |
|||
MBS319482-01mg | MyBiosource | 0.1mg | EUR 290 |
hCG >=98% Pure |
|||
MBS319482-5x01mg | MyBiosource | 5x0.1mg | EUR 1135 |
DNA Pure Kit |
|||
FYG601-100P | Yeastern Biotech | 100 Preps | Ask for price |
DNA Pure Kit |
|||
FYG601-300P | Yeastern Biotech | 300 Preps | Ask for price |
RNA Pure Kit |
|||
FYG602-100P | Yeastern Biotech | 100 Preps | Ask for price |
RNA Pure Kit |
|||
FYG602-50P | Yeastern Biotech | 50 Preps | Ask for price |
Agar PT, Pure |
|||
RM7695-1KG | EWC Diagnostics | 1 unit | EUR 150.38 |
Description: Agar PT, Pure |
Agar PT, Pure |
|||
RM7695-500G | EWC Diagnostics | 1 unit | EUR 84 |
Description: Agar PT, Pure |
Bees Wax pure |
|||
24031 | Sisco Laboratories | 500 Gms | EUR 5.47 |
Description: Part A |
Xylene pure, 99% |
|||
54717 | Sisco Laboratories | 500 ml | EUR 2.46 |
Description: Part A |
Citral pure, 95% |
|||
29965 | Sisco Laboratories | 100 ml | EUR 4.35 |
Description: Part B |
Almond Oil pure |
|||
45262 | Sisco Laboratories | 500 ml | EUR 46.59 |
Description: Part A |
Pinacol pure, 98% |
|||
99487 | Sisco Laboratories | 25 Gms | EUR 13.35 |
Description: Part B |
Camphor pure, 96% |
|||
64813 | Sisco Laboratories | 100 Gms | EUR 3.83 |
Description: Part A |
Toluene pure, 99% |
|||
47556 | Sisco Laboratories | 500 ml | EUR 1.95 |
Description: Part A |
Cuelure pure, 95% |
|||
12295 | Sisco Laboratories | 25 Gms | EUR 5.63 |
Description: Part B |
Glycine pure, 99% |
|||
25853 | Sisco Laboratories | 500 Gms | EUR 3.97 |
Description: Part A |
Castor Oil pure |
|||
16145 | Sisco Laboratories | 500 ml | EUR 4.65 |
Description: Part A |
C1q highly pure |
|||
GWB-F38918 | GenWay Biotech | 1 mg | Ask for price |
Calamine pure, 98% |
|||
44509 | Sisco Laboratories | 500 Gms | EUR 4.45 |
Description: Part A |
Starch Corn pure |
|||
58254 | Sisco Laboratories | 500 Gms | EUR 1.51 |
Description: Part A |
Ajmaline pure, 98% |
|||
52821 | Sisco Laboratories | 50 Mg | EUR 60 |
Description: Part B |
Methanol pure, 99% |
|||
59029 | Sisco Laboratories | 500 ml | EUR 1.71 |
Description: Part A |
Anthrone pure, 95% |
|||
59553 | Sisco Laboratories | 25 Gms | EUR 4.5 |
Description: Part B |
Thiourea pure, 98% |
|||
88196 | Sisco Laboratories | 500 Gms | EUR 6.84 |
Description: Part A |
Inositol pure, 99% |
|||
98211 | Sisco Laboratories | 25 Gms | EUR 2.7 |
Description: Part B |
Vanillin pure, 99% |
|||
98842 | Sisco Laboratories | 25 Gms | EUR 2.19 |
Description: Part A |
Hexamine pure, 99% |
|||
81451 | Sisco Laboratories | 500 Gms | EUR 2.6 |
Description: Part A |
Iodoform pure, 99% |
|||
36388 | Sisco Laboratories | 25 Gms | EUR 10.95 |
Description: Part A |
Coumarin pure, 98% |
|||
42074 | Sisco Laboratories | 100 Gms | EUR 4.5 |
Description: Part B |
CHAPS, Ultra Pure |
|||
22024-100G | Biotium | 100G | EUR 667 |
Description: N/A |
CHAPS, Ultra Pure |
|||
22024-100G-1 | Biotium | EA | EUR 667 |
CHAPS, Ultra Pure |
|||
22024-10G | Biotium | 10G | EUR 108 |
Description: N/A |
CHAPS, Ultra Pure |
|||
22024-10G-1 | Biotium | EA | EUR 108 |
Naringin pure, 97% |
|||
27734 | Sisco Laboratories | 5 Gms | EUR 8.25 |
Description: Part B |
Arachis Oil pure |
|||
24766 | Sisco Laboratories | 250 ml | EUR 8.76 |
Description: Part A |
Carbinol pure, 99% |
|||
15343 | Sisco Laboratories | 500 ml | EUR 1.71 |
Description: Part A |
Pectic Acid pure |
|||
13684 | Sisco Laboratories | 100 Gms | EUR 27.75 |
Description: Part B |
CHAPS, Ultra Pure |
|||
C0660-005 | GenDepot | 5g | EUR 90 |
CHAPS, Ultra Pure |
|||
C0660-010 | GenDepot | 10g | EUR 160 |
Water, Ultra Pure |
|||
IBS-BW006 | iNtRON Biotechnology Inc | 1L | EUR 21 |
Water, Ultra pure |
|||
IBS-BW006-2 | iNtRON Biotechnology Inc | 250 mL | EUR 8.4 |
Water, Ultra Pure |
|||
IBS-BW006a | iNtRON Biotechnology Inc | 500 mL | EUR 14 |
Water, Ultra pure |
|||
IBS-BW010 | iNtRON Biotechnology Inc | 1 mL x 100 | EUR 105 |
Curcumin, pure, 98% |
|||
GP7022 | Glentham Life Sciences | 10mg | EUR 326.74 |
Curcumin, pure, 98% |
|||
GP7022-10 | Glentham Life Sciences | 10 | EUR 72.2 |
Curcumin, pure, 98% |
|||
GP7022-10MG | Glentham Life Sciences | 10 mg | EUR 123.6 |
Curcumin, pure, 98% |
|||
GP7022-250 | Glentham Life Sciences | 250 | EUR 356.8 |
Curcumin, pure, 98% |
|||
GP7022-250MG | Glentham Life Sciences | 250 mg | EUR 468 |
Curcumin, pure, 98% |
|||
GP7022-50 | Glentham Life Sciences | 50 | EUR 129.2 |
Curcumin, pure, 98% |
|||
GP7022-50MG | Glentham Life Sciences | 50 mg | EUR 192 |
Magon, Extra pure |
|||
RM846-1G | EWC Diagnostics | 1 unit | EUR 89.26 |
Description: Magon, Extra pure |
Carbazole pure, 95% |
|||
54338 | Sisco Laboratories | 100 Gms | EUR 15 |
Description: Part B |
Phthalide pure, 98% |
|||
45501 | Sisco Laboratories | 100 Gms | EUR 3.98 |
Description: Part B |
Harmaline pure, 98% |
|||
52293 | Sisco Laboratories | 1 Gms | EUR 36.45 |
Description: Part B |
Formamide pure, 98.5% |
|||
53072 | Sisco Laboratories | 500 ml | EUR 3.01 |
Description: Part A |
Benzamide pure, 98% |
|||
87688 | Sisco Laboratories | 100 Gms | EUR 2.05 |
Description: Part A |
Dithizone pure, 70% |
|||
97980 | Sisco Laboratories | 5 Gms | EUR 6.15 |
Description: Part B |
Acetamide pure, 98% |
|||
95237 | Sisco Laboratories | 500 Gms | EUR 5.13 |
Description: Part A |
Veratrole pure, 98% |
|||
96011 | Sisco Laboratories | 100 ml | EUR 4.95 |
Description: Part B |
Quinoline pure, 97% |
|||
32648 | Sisco Laboratories | 500 ml | EUR 13.8 |
Description: Part B |
Imidazole pure, 99% |
|||
65712 | Sisco Laboratories | 100 Gms | EUR 2.53 |
Description: Part A |
Ethyl Oleate pure |
|||
70945 | Sisco Laboratories | 500 ml | EUR 4.88 |
Description: Part B |
Triacetin pure, 99% |
|||
73222 | Sisco Laboratories | 500 ml | EUR 5.54 |
Description: Part A |
CHAPSO, Ultra Pure |
|||
C0661-001 | GenDepot | 1g | EUR 195 |
BSA, affinity pure |
|||
RP-4034-30 | ImmunoBioscience | 2.5 mg | EUR 79.65 |
Lanosterol pure, ~60% |
|||
56839 | Sisco Laboratories | 1 Gms | EUR 8.7 |
Description: Part B |
Oleylamine pure, 95% |
|||
87519 | Sisco Laboratories | 250 ml | EUR 2.67 |
Description: Part A |
Picrotoxin pure, 98% |
|||
93418 | Sisco Laboratories | 1 Gms | EUR 15.83 |
Description: Part B |
Naringenin pure, 95% |
|||
95956 | Sisco Laboratories | 1 Gms | EUR 10.58 |
Description: Part B |
Isodecanol pure, 98% |
|||
90189 | Sisco Laboratories | 500 ml | EUR 8.1 |
Description: Part B |
Riboflavin pure, 98% |
|||
34392 | Sisco Laboratories | 10 Gms | EUR 2.4 |
Description: Part B |
Pyridazine pure, 98% |
|||
67172 | Sisco Laboratories | 5 Gms | EUR 20.48 |
Description: Part B |
Allylamine pure, 99% |
|||
69098 | Sisco Laboratories | 25 ml | EUR 6.91 |
Description: Part A |
Potato Starch pure |
|||
69285 | Sisco Laboratories | 500 Gms | EUR 2.46 |
Description: Part A |
Isobutanol pure, 99% |
|||
69412 | Sisco Laboratories | 500 ml | EUR 2.46 |
Description: Part A |
Ichthammol pure, 50% |
|||
64556 | Sisco Laboratories | 500 Gms | EUR 39 |
Description: Part A |
Agarose Ultra Pure |
|||
BA01101 | Bioatlas | 100g | EUR 147.6 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Agarose Ultra Pure |
|||
BA01102 | Bioatlas | 500g | EUR 399.6 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Pyrrolidine pure, 99% |
|||
91402 | Sisco Laboratories | 25 ml | EUR 1.88 |
Description: Part B |
Benzylamine pure, 99% |
|||
38728 | Sisco Laboratories | 500 ml | EUR 6.15 |
Description: Part B |
Benzanilide pure, 98% |
|||
13740 | Sisco Laboratories | 100 Gms | EUR 4.43 |
Description: Part B |
Methylene blue, Pure |
|||
GRM956-100G | EWC Diagnostics | 1 unit | EUR 18.62 |
Description: Methylene blue, Pure |
Methylene blue, Pure |
|||
GRM956-1KG | EWC Diagnostics | 1 unit | EUR 167.54 |
Description: Methylene blue, Pure |
Methylene blue, Pure |
|||
GRM956-25G | EWC Diagnostics | 1 unit | EUR 6.93 |
Description: Methylene blue, Pure |
Nitrobenzene pure, 98% |
|||
55410 | Sisco Laboratories | 500 ml | EUR 2.33 |
Description: Part A |
Paraffin Yellow pure |
|||
60536 | Sisco Laboratories | 500 Gms | EUR 4.24 |
Description: Part A |
Salicylamide pure, 98% |
|||
77386 | Sisco Laboratories | 500 Gms | EUR 13.13 |
Description: Part B |
Benzaldehyde pure, 99% |
|||
37278 | Sisco Laboratories | 500 ml | EUR 3.42 |
Description: Part A |
Silver Vanadate pure |
|||
69717 | Sisco Laboratories | 5 Gms | EUR 27 |
Description: Part B |
Emulsifying Wax pure |
|||
71867 | Sisco Laboratories | 500 Gms | EUR 4.17 |
Description: Part A |
Diethylamine pure, 99% |
|||
31255 | Sisco Laboratories | 500 ml | EUR 2.39 |
Description: Part A |
Hydroquinone pure, 98% |
|||
28449 | Sisco Laboratories | 100 Gms | EUR 3.38 |
Description: Part B |
Avidin, affinity pure |
|||
GP-4078-30 | ImmunoBioscience | 1.0 mg | EUR 88.2 |
Paclobutrazol pure, 95% |
|||
57590 | Sisco Laboratories | 5 Gms | EUR 6.75 |
Description: Part B |
Fluorobenzene pure, 99% |
|||
88879 | Sisco Laboratories | 100 ml | EUR 7.5 |
Description: Part B |
Benzotriazole pure, 97% |
|||
98101 | Sisco Laboratories | 100 Gms | EUR 3.08 |
Description: Part A |
Dicyandiamide pure, 98% |
|||
77833 | Sisco Laboratories | 500 Gms | EUR 5.13 |
Description: Part A |
Dibenzylamine pure, 98% |
|||
23144 | Sisco Laboratories | 100 ml | EUR 6.45 |
Description: Part B |
Propiophenone pure, 99% |
|||
41622 | Sisco Laboratories | 100 ml | EUR 7.35 |
Description: Part B |
Anthraquinone pure, 98% |
|||
78531 | Sisco Laboratories | 25 Gms | EUR 2.33 |
Description: Part A |
Triethylamine pure, 98% |
|||
29306 | Sisco Laboratories | 500 ml | EUR 2.74 |
Description: Part A |
Furfurylamine pure, 98% |
|||
11049 | Sisco Laboratories | 500 ml | EUR 11.1 |
Description: Part B |
Octadecylamine pure, 98% |
|||
51038 | Sisco Laboratories | 250 Gms | EUR 4.5 |
Description: Part B |
Sulphanilamide pure, 99% |
|||
89762 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
Piperonylamine pure, 97% |
|||
31826 | Sisco Laboratories | 5 ml | EUR 52.5 |
Description: Part B |
Menthol Synthetic pure |
|||
64370 | Sisco Laboratories | 100 Gms | EUR 6.5 |
Description: Part A |
Hydrazobenzene pure, 97% |
|||
40935 | Sisco Laboratories | 25 Gms | EUR 4.05 |
Description: Part B |
Podophyllum Resin pure |
|||
41175 | Sisco Laboratories | 25 Gms | EUR 41.67 |
Description: Part A |
Syringaldazine pure, 95% |
|||
47406 | Sisco Laboratories | 1 Gms | EUR 33.75 |
Description: Part B |
Diethanolamine pure, 98% |
|||
71164 | Sisco Laboratories | 500 ml | EUR 2.39 |
Description: Part A |
Hydrocortisone pure, 97% |
|||
13542 | Sisco Laboratories | 1 Gms | EUR 3.3 |
Description: Part B |
Benzhydrylamine pure, 97% |
|||
57730 | Sisco Laboratories | 25 ml | EUR 9 |
Description: Part B |
Outcomes: The abundance of miR-204-5p was a lot increased in lactating mouse mammary glands than in different tissues, which indicated that miR-204-5p could also be concerned in regulating milk manufacturing. MiR-204-5p affected the expression of β-casein and milk lipid synthesis in HC11 mouse mammary epithelial cells however didn’t affect the proliferation of HC11 cells. Overexpression of miR-204-5p significantly elevated the variety of Oil Pink O+ cells, triglyceride accumulation and the expression of markers related to lipid synthesis, together with FASN and PPARγ, whereas inhibition of miR-204-5p had the other impact. miR-204-5p promotes lipid synthesis by negatively regulating SIRT1. Overexpression of SIRT1 can repress the promotion of miR-204-5p on lipid synthesis.