December 23, 2020
CCAT2 contributes to progression and treatment resistance of thyroid carcinoma
Goal: The intention of this research is to uncover the correlations of the expression of colon most cancers related transcript 2 (CCAT2) within the medical papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC) specimens with the prognosis and chemoresistance of sufferers.
Sufferers and strategies: The expression degree of CCAT2 within the PTC and ATC specimens was decided utilizing Actual-Time quantitative Polymerase Chain Response (RT-qPCR), and the correlations of CCAT2 expression with the medical options of sufferers had been detected through χ2 check. Apart from, survival evaluation was carried out to confirm the relation between CCAT2 expression and sufferers’ survival. After knockdown or overexpression of CCAT2, the adjustments within the proliferation skill of human thyroid carcinoma cells had been examined through Cell Counting package-8 (CCK-8) assay, and the half maximal inhibitory focus (IC50) values of doxorubicin and cisplatin had been measured by methyl thiazolyl tetrazolium (MTT) assay.
Outcomes: In line with the χ2-test outcomes, the expression of CCAT2 was notably correlated with the capsular invasion and lymph node metastasis of PTC, and the capsular invasion, tumor dimension, and lymph node metastasis of ATC. It was found via the survival evaluation that the expression of CCAT2 was notably related to the poor prognosis of ATC sufferers. After knockdown of CCAT2, each the proliferation skill and the IC50 values of doxorubicin and cisplatin considerably declined in human thyroid carcinoma cells. The other situations had been discovered after CCAT2 was overexpressed in human thyroid carcinoma cells.
Conclusions: CCAT2 potentiates the proliferation skill and chemoresistance of cells, promotes the development of thyroid carcinoma, and hinders the prognosis of ATC.
Isolation and Characterization of Coronavirus and Rotavirus Related to Calves in Central A part of Oromia, Ethiopia
Background: Coronavirus and rotavirus are mostly related etiologies for calves’ diarrhoea, leading to lack of productiveness and financial system of farmers. Nonetheless, varied sides of diarrheal illness attributable to coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross-sectional research was carried out with the intention of isolation and molecular characterization of coronavirus and rotavirus from calves within the central a part of Oromia (Bishoftu, Sebata, Holeta, and Addis Ababa), Ethiopia, from November 2018 to Could 2019. The 4 research areas had been purposively chosen and faecal samples had been collected by easy random sampling for analysis of coronavirus and rotavirus an infection by utilizing the antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) package. As well as, this research was carried out to have perception in prevalence and related danger components of coronavirus and rotavirus an infection in calves.
End result: Throughout the research, 83 diarrheic and 162 nondiarrheic faecal samples collected from calves lower than Four weeks of age had been screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 pattern (1.2%) was constructive for coronavirus antigen and 6 samples (7.2%) had been discovered to be constructive for rotavirus antigen by Ag-ELISA. All of the nondiarrheic samples had been unfavorable for each coronavirus and rotavirus Ag.
The general prevalence of coronavirus and rotavirus an infection in calves was estimated at 0.4% (1/245) and a couple of.45% (6/245), respectively. All samples (7) of ELISA check constructive of each coronavirus and rotavirus had been propagated in Madin-Darby bovine kidney (MDBK) cells. After three subsequent passages, progressive cytopathic impact (CPE), i.e., rounding, detachment, and the destruction of monolayer cell of 5 samples (1 pattern of coronavirus and Four samples of rotavirus) (71.4%) had been noticed. On the molecular stage, reverse transcriptase polymerase chain response (RT-PCR) method was used to find out the presence of coronavirus and rotavirus nucleic acid by utilizing particular primers. The 5 samples that had been coronavirus and rotavirus antigen constructive by ELISA and develop CPE on cell tradition had been additionally constructive on RT-PCR method. The prevalence of an infection peaked at 1st and 2nd weeks of age in male calves.
Conclusion: Diarrheal illness attributable to coronavirus and rotavirus has an incredible well being drawback in calves that interrupts manufacturing advantages with diminished weight acquire and elevated mortality and its potential for zoonotic unfold. So, the current findings present coronavirus and rotavirus an infection in calves in Ethiopia that must be addressed by practising early colostrum feeding in new child calves, utilizing vaccine, or bettering livestock administration.
MiR-204-5p promotes lipid synthesis in mammary epithelial cells by concentrating on SIRT1
Aims: Understanding the molecular mechanisms of lipid synthesis within the mammary gland is essential for regulating the extent and composition of lipids in milk. This research aimed to analyze the purposeful and molecular mechanisms of miR-204-5p in mammary epithelial cells to offer a theoretical foundation for milk lipid synthesis.
Strategies: Actual-time quantitative PCR was carried out to detect the transcriptional ranges of miR-204-5p and associated mRNA abundance in mammary epithelial cells. Western blotting was carried out to find out protein expression. Cell proliferation was assessed by Cell Counting Equipment-8. A dual-luciferase reporter assay was carried out to confirm the concentrating on relationship between miR-204-5p and SIRT1. siRNA and overexpression plasmids had been transfected into mouse HC11 mammary epithelial cells.
HiCaN Pure |
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PGE0016-1KG | EWC Diagnostics | 1 unit | EUR 6.19 |
Description: HiCaN Pure |
HiCaN Pure |
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PGE0016-500G | EWC Diagnostics | 1 unit | EUR 3.18 |
Description: HiCaN Pure |
HiPoT Pure |
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PGE0017-1KG | EWC Diagnostics | 1 unit | EUR 12.37 |
Description: HiPoT Pure |
HiPoT Pure |
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PGE0017-500G | EWC Diagnostics | 1 unit | EUR 8.1 |
Description: HiPoT Pure |
HiBoR Pure |
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PGE0018-500G | EWC Diagnostics | 1 unit | EUR 7.88 |
Description: HiBoR Pure |
Oxyma Pure |
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abx095068-100l | Abbexa | 100 µl | EUR 250 |
Oxyma Pure |
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abx095068-1ml | Abbexa | 1 ml | EUR 775 |
Oxyma Pure |
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abx095068-200l | Abbexa | 200 µl | EUR 525 |
Aluminon, Pure |
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RM283-100G | EWC Diagnostics | 1 unit | EUR 141.11 |
Description: Aluminon, Pure |
Aluminon, Pure |
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RM283-25G | EWC Diagnostics | 1 unit | EUR 40.36 |
Description: Aluminon, Pure |
Acetamide, pure |
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GL1221-1 | Glentham Life Sciences | 1 | EUR 39.4 |
Acetamide, pure |
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GL1221-1KG | Glentham Life Sciences | 1 kg | EUR 84 |
Acetamide, pure |
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GL1221-250 | Glentham Life Sciences | 250 | EUR 15.9 |
Acetamide, pure |
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GL1221-250G | Glentham Life Sciences | 250 g | EUR 55.2 |
Acetamide, pure |
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GL1221-5 | Glentham Life Sciences | 5 | EUR 126.5 |
Acetamide, pure |
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GL1221-5KG | Glentham Life Sciences | 5 kg | EUR 189.6 |
Plasminogen Pure |
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GWB-248041 | GenWay Biotech | 1 mg | Ask for price |
FSH 98% Pure |
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GWB-DA26DC | GenWay Biotech | 0.1 mg | Ask for price |
hCG 98% pure |
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GWB-BEBACF | GenWay Biotech | 0.1 mg | Ask for price |
PSA 98% Pure |
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GWB-BFDD4C | GenWay Biotech | 1 mg | Ask for price |
DNA Pure Kit |
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FYG601-100P | Yeastern Biotech | 100 Preps | Ask for price |
DNA Pure Kit |
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FYG601-300P | Yeastern Biotech | 300 Preps | Ask for price |
RNA Pure Kit |
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FYG602-100P | Yeastern Biotech | 100 Preps | Ask for price |
RNA Pure Kit |
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FYG602-50P | Yeastern Biotech | 50 Preps | Ask for price |
Agar PT, Pure |
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RM7695-1KG | EWC Diagnostics | 1 unit | EUR 150.38 |
Description: Agar PT, Pure |
Agar PT, Pure |
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RM7695-500G | EWC Diagnostics | 1 unit | EUR 84 |
Description: Agar PT, Pure |
C1q highly pure |
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GWB-F38918 | GenWay Biotech | 1 mg | Ask for price |
CHAPS, Ultra Pure |
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22024-100G | Biotium | 100G | EUR 667 |
Description: N/A |
CHAPS, Ultra Pure |
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22024-100G-1 | Biotium | EA | EUR 667 |
CHAPS, Ultra Pure |
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22024-10G | Biotium | 10G | EUR 108 |
Description: N/A |
CHAPS, Ultra Pure |
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22024-10G-1 | Biotium | EA | EUR 108 |
CHAPS, Ultra Pure |
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C0660-005 | GenDepot | 5g | EUR 198 |
CHAPS, Ultra Pure |
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C0660-010 | GenDepot | 10g | EUR 303.6 |
Curcumin, pure, 98% |
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GP7022-10 | Glentham Life Sciences | 10 | EUR 72.2 |
Curcumin, pure, 98% |
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GP7022-10MG | Glentham Life Sciences | 10 mg | EUR 123.6 |
Curcumin, pure, 98% |
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GP7022-250 | Glentham Life Sciences | 250 | EUR 356.8 |
Curcumin, pure, 98% |
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GP7022-250MG | Glentham Life Sciences | 250 mg | EUR 468 |
Curcumin, pure, 98% |
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GP7022-50 | Glentham Life Sciences | 50 | EUR 129.2 |
Curcumin, pure, 98% |
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GP7022-50MG | Glentham Life Sciences | 50 mg | EUR 192 |
Magon, Extra pure |
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RM846-1G | EWC Diagnostics | 1 unit | EUR 89.26 |
Description: Magon, Extra pure |
CHAPSO, ultra Pure |
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C0661-001 | GenDepot | 1g | EUR 198 |
BSA, affinity pure |
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RP-4034-30 | ImmunoBioscience | 2.5 mg | EUR 79.65 |
Agarose Ultra Pure |
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BA01101 | Bioatlas | 100g | EUR 147.6 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Agarose Ultra Pure |
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BA01102 | Bioatlas | 500g | EUR 399.6 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Methylene blue, Pure |
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GRM956-100G | EWC Diagnostics | 1 unit | EUR 18.62 |
Description: Methylene blue, Pure |
Methylene blue, Pure |
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GRM956-1KG | EWC Diagnostics | 1 unit | EUR 167.54 |
Description: Methylene blue, Pure |
Methylene blue, Pure |
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GRM956-25G | EWC Diagnostics | 1 unit | EUR 6.93 |
Description: Methylene blue, Pure |
Avidin, affinity pure |
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GP-4078-30 | ImmunoBioscience | 1.0 mg | EUR 88.2 |
CK-MB 90% Pure |
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GWB-3161F3 | GenWay Biotech | 1 mg | Ask for price |
Human TSH 98% Pure |
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GWB-1E5CED | GenWay Biotech | 0.1 mg | Ask for price |
RNA PURE KIT - 4rxn |
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IB47640 | IBI Scientific | - | EUR 30.11 |
Human TSH 95% Pure |
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GWB-C51D9D | GenWay Biotech | 0.05 mg | Ask for price |
SDS, 99% Ultra Pure |
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S0800-100 | GenDepot | 1Kg | EUR 375.6 |
SDS, 99% Ultra Pure |
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S0800-250 | GenDepot | 2.5 Kg | EUR 643.2 |
SDS, 99% Ultra Pure |
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S0800-500 | GenDepot | 5 Kg | EUR 1146 |
Ultra Pure ADP 1ml |
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ADP100-2 | Cell Technology | 1mL | EUR 380 |
Ultra Pure ADP 5ml |
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ADP100-3 | Cell Technology | 5mL | EUR 1590 |
RNA PURE KIT - 50rxn |
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IB47641 | IBI Scientific | - | EUR 205.96 |
RNA PURE KIT - 100rxn |
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IB47642 | IBI Scientific | - | EUR 350.13 |
DTT, Ultra Pure, 15g |
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R7634 | ACTGene | 15g | EUR 121.44 |
DTT, Ultra Pure, 15g |
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R7634-15g | ACTGene | each | EUR 228 |
DTT, Ultra Pure, 50g |
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R7634-50g | ACTGene | each | EUR 531.6 |
PAP protein (> 30% pure) |
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30-1026 | Fitzgerald | 100 units | EUR 142 |
Description: Purified native Human PAP protein |
PAP protein (> 98% pure) |
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30-1027 | Fitzgerald | 1 kU | EUR 444 |
Description: Purified native Human PAP protein |
PSA protein (> 98% pure) |
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30-1205 | Fitzgerald | 100 ug | EUR 355 |
Description: Purified native Human Prostate Specific Antigen protein |
TBG protein (> 70% pure) |
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30-1213 | Fitzgerald | 1 mg | EUR 275 |
Description: Purified native Human TBG protein |
TBG protein (> 98% pure) |
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30-1214 | Fitzgerald | 1 mg | EUR 823.2 |
Description: Purified native Human TBG protein |
CRP protein (>80% pure) |
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30-AC05PP | Fitzgerald | 5 mg | EUR 250 |
Description: Partially purified native Human CRP protein |
CRP protein (>95% pure) |
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30-AC05S | Fitzgerald | 5 mg | EUR 250 |
Description: Purified native Human CRP protein |
PSA protein (> 75% pure) |
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30-AP16S | Fitzgerald | 100 ug | EUR 152 |
Description: Purified native Human PSA protein |
TSH protein (> 98% pure) |
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30-AT05 | Fitzgerald | 100 ug | EUR 1250 |
Description: Purified native Human TSH protein (> 98% pure) |
TSH protein (>98% pure) |
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30-AT05-1 | Fitzgerald | 1 mg | EUR 3900 |
Description: Purified native Human TSH protein (>98% pure) |
TSH protein (> 95% pure) |
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30-AT10 | Fitzgerald | 100 ug | EUR 1250 |
Description: Purified native Human TSH protein (> 95% pure) |
TBG protein (> 98% pure) |
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30-AT30 | Fitzgerald | 1 mg | Ask for price |
Description: Purified native Human TBG protein |
TBG protein (> 98% pure) |
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30-AT30S | Fitzgerald | 1 mg | EUR 253 |
Description: Purified native Human TBG protein |
TBG protein (> 80% pure) |
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30-AT35 | Fitzgerald | 1 mg | EUR 195 |
Description: Purified native Human TBG protein |
RBP protein (> 95% pure) |
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30R-AR022 | Fitzgerald | 100 ug | EUR 100 |
Description: Highly purified native Human RBP protein |
RBP protein (95% pure) |
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30R-AR022L | Fitzgerald | 1 mg | EUR 274 |
Description: Highly purified native Human RBP protein |
TSH protein (> 50% pure) |
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30R-AT009 | Fitzgerald | 1 mg | EUR 1066.8 |
Description: Purified native Human TSH protein (> 50% pure) |
PAP protein (> 95% pure) |
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30C-CP1016 | Fitzgerald | 1 mg | EUR 360 |
Description: Purified native Human PAP protein (> 95% pure) |
PAP protein (> 40% pure) |
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30C-CP1016P | Fitzgerald | 1 mg | EUR 93 |
Description: Purified native Human PAP protein (> 40% pure) |
PSA protein (> 95% pure) |
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30C-CP1017 | Fitzgerald | 1 mg | EUR 548.4 |
Description: Purified native Human PSA protein (active enzyme) |
PSA protein (> 40% pure) |
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30C-CP1017P | Fitzgerald | 1 mg | EUR 194 |
Description: Purified native Human PSA protein |
PSA protein (> 98% pure) |
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30C-CP1017U | Fitzgerald | 1 mg | EUR 678 |
Description: Purified native Human PSA protein |
RBP protein (> 40% pure) |
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30C-CP1024P | Fitzgerald | 1 mg | EUR 50 |
Description: Purified native Human RBP protein (> 40% pure) |
TBG protein (> 95% pure) |
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30C-CP1025 | Fitzgerald | 1 mg | EUR 135 |
Description: Purified native Human TBG protein (> 95% pure) |
TBG protein (> 40% pure) |
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30C-CP1025P | Fitzgerald | 1 mg | EUR 99 |
Description: Purified native Human TBG protein (> 40% pure) |
TSH protein (>50% pure) |
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30C-CP1026P | Fitzgerald | 1 mg | EUR 1066.8 |
Description: Purified native Human TSH protein (>50% pure) |
PSA protein (> 95% pure) |
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30C-CP1099 | Fitzgerald | 100 ug | EUR 361.2 |
Description: Purified native Human PSA protein |
PSA protein (> 95% pure) |
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30C-CP1105 | Fitzgerald | 100 ug | EUR 371 |
Description: Purified native Human PSA protein |
PSA protein (> 95% pure) |
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30C-CP1118 | Fitzgerald | 100 ug | EUR 268.8 |
Description: Purified native Human PSA protein |
EDTA, Ultra Pure, 1kg |
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R9410 | ACTGene | 1kg | EUR 121.44 |
EDTA, Ultra Pure, 1kg |
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R9410-1kg | ACTGene | each | EUR 228 |
DTT, Ultra Pure Grade |
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40400138-1 | Glycomatrix | 5 g | EUR 148.25 |
DTT, Ultra Pure Grade |
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40400138-2 | Glycomatrix | 10 g | EUR 266.84 |
DTT, Ultra Pure Grade |
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40400138-3 | Glycomatrix | 25 g | EUR 444.73 |
DTT, Ultra Pure Grade |
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40400138-4 | Glycomatrix | 50 g | EUR 667.09 |
DTT, Ultra Pure Grade |
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40400138-5 | Glycomatrix | 100 g | EUR 1037.7 |
DTT, Ultra Pure Grade |
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40400138-6 | Glycomatrix | 500 g | EUR 4076.66 |
Stearic acid, pure, 98% |
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GK7312-10 | Glentham Life Sciences | 10 | EUR 22.1 |
Stearic acid, pure, 98% |
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GK7312-100 | Glentham Life Sciences | 100 | EUR 102.7 |
Stearic acid, pure, 98% |
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GK7312-100G | Glentham Life Sciences | 100 g | EUR 160.8 |
Stearic acid, pure, 98% |
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GK7312-10G | Glentham Life Sciences | 10 g | EUR 62.4 |
Stearic acid, pure, 98% |
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GK7312-25 | Glentham Life Sciences | 25 | EUR 35.5 |
Stearic acid, pure, 98% |
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GK7312-25G | Glentham Life Sciences | 25 g | EUR 79.2 |
Stearic acid, pure, 98% |
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GK7312-50 | Glentham Life Sciences | 50 | EUR 55.4 |
Stearic acid, pure, 98% |
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GK7312-50G | Glentham Life Sciences | 50 g | EUR 103.2 |
Tris Base, Ultra Pure |
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T9200-100 | GenDepot | 1kg | EUR 200.4 |
Tris Base , Ultra Pure |
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T9200-500 | GenDepot | 5kg | EUR 619.2 |
Methyl Green Pure C.I. No. 42585 |
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M18710 | Pfaltz & Bauer | 1G | EUR 92.6 |
IPTG, Ultra Pure Grade |
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21530112-1 | Glycomatrix | 1 g | EUR 38.75 |
IPTG, Ultra Pure Grade |
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21530112-2 | Glycomatrix | 5 g | EUR 80.12 |
IPTG, Ultra Pure Grade |
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21530112-3 | Glycomatrix | 25 g | EUR 267.76 |
IPTG, Ultra Pure Grade |
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21530112-4 | Glycomatrix | 50 g | EUR 476.53 |
IPTG, Ultra Pure Grade |
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21530112-5 | Glycomatrix | 100 g | EUR 915.76 |
CAPS, Ultra Pure Grade |
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40320007-1 | Glycomatrix | 25 g | EUR 22.69 |
CAPS, Ultra Pure Grade |
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40320007-2 | Glycomatrix | 100 g | EUR 39.41 |
CAPS, Ultra Pure Grade |
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40320007-3 | Glycomatrix | 250 g | EUR 91.39 |
CAPS, Ultra Pure Grade |
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40320007-4 | Glycomatrix | 500 g | EUR 158.6 |
CAPS, Ultra Pure Grade |
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40320007-5 | Glycomatrix | 1 kg | EUR 282.81 |
Tris, Ultra Pure Grade |
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42020236-1 | Glycomatrix | 500 g | EUR 35.14 |
Tris, Ultra Pure Grade |
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42020236-2 | Glycomatrix | 1 kg | EUR 64.93 |
Urea, Ultra Pure Grade |
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42100004-1 | Glycomatrix | 500 g | EUR 29.29 |
Urea, Ultra Pure Grade |
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42100004-2 | Glycomatrix | 1 kg | EUR 45.58 |
Urea, Ultra Pure Grade |
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42100004-3 | Glycomatrix | 2.5 kg | EUR 90.2 |
Tris - Ultra Pure Grade |
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TB0194 | Bio Basic | 500g | EUR 89.23 |
Geneticin (G418, (>98% pure)) |
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ABT-800-01 | Alpha Diagnostics | 1 g | EUR 196.8 |
Geneticin (G418, (>98% pure)) |
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ABT-800-05 | Alpha Diagnostics | 5 g | EUR 489.6 |
Agar Powder, Extra pure |
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RM301-1KG | EWC Diagnostics | 1 unit | EUR 249.49 |
Description: Agar Powder, Extra pure |
Agar Powder, Extra pure |
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RM301-500G | EWC Diagnostics | 1 unit | EUR 129.36 |
Description: Agar Powder, Extra pure |
Uracil, Ultra Pure Grade |
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42190000-1 | Glycomatrix | 250 g | EUR 61.76 |
Uracil, Ultra Pure Grade |
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42190000-2 | Glycomatrix | 500 g | EUR 109.81 |
Tannic acid powder, Pure |
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GRM7541-100G | EWC Diagnostics | 1 unit | EUR 19.53 |
Description: Tannic acid powder, Pure |
Tannic acid powder, Pure |
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GRM7541-250G | EWC Diagnostics | 1 unit | EUR 42.93 |
Description: Tannic acid powder, Pure |
Tannic acid powder, Pure |
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GRM7541-500G | EWC Diagnostics | 1 unit | EUR 77.01 |
Description: Tannic acid powder, Pure |
Water, Ultra Pure, Sterile |
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18-193 | Genesee Scientific | 100ml/Unit | EUR 20.42 |
Description: Molecular Biology Grade |
Water, Ultra Pure, Sterile |
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18-194 | Genesee Scientific | 500ml/Unit | EUR 47.29 |
Description: Molecular Biology Grade |
Water, Ultra Pure, Sterile |
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18-195 | Genesee Scientific | 1000ml/Unit | EUR 70.65 |
Description: Molecular Biology Grade |
Water, Ultra Pure, Sterile |
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18-196 | Genesee Scientific | 4 x 1L/Unit | EUR 184.04 |
Description: Molecular Biology Grade |
Sucrose, Ultra Pure Grade |
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41900152-1 | Glycomatrix | 500 g | EUR 18.77 |
Sucrose, Ultra Pure Grade |
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41900152-2 | Glycomatrix | 1 kg | EUR 26.06 |
Sucrose, Ultra Pure Grade |
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41900152-3 | Glycomatrix | 5 kg | EUR 121.96 |
Aprotinin, bovine (pure >98%) |
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RP-1532 | Alpha Diagnostics | 100 mg | EUR 343.2 |
Thioflavin T, Ultra Pure |
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T1002-001 | GenDepot | 1g | EUR 343.2 |
Prolactin protein > 95% pure |
|||
30-1199 | Fitzgerald | 100 ug | Ask for price |
Description: Purified native Human Prolactin protein |
Prolactin protein > 50% pure |
|||
30-1200 | Fitzgerald | 100 ug | Ask for price |
Description: Purified native Human Prolactin protein |
Prolactin protein (> 98% pure) |
|||
30-AP05 | Fitzgerald | 100 ug | EUR 1900 |
Description: Purified native Human Prolactin protein (> 98% pure) |
Outcomes: The abundance of miR-204-5p was a lot increased in lactating mouse mammary glands than in different tissues, which indicated that miR-204-5p could also be concerned in regulating milk manufacturing. MiR-204-5p affected the expression of β-casein and milk lipid synthesis in HC11 mouse mammary epithelial cells however didn’t affect the proliferation of HC11 cells. Overexpression of miR-204-5p significantly elevated the variety of Oil Pink O+ cells, triglyceride accumulation and the expression of markers related to lipid synthesis, together with FASN and PPARγ, whereas inhibition of miR-204-5p had the other impact. miR-204-5p promotes lipid synthesis by negatively regulating SIRT1. Overexpression of SIRT1 can repress the promotion of miR-204-5p on lipid synthesis.