Glial cell induced neural differentiation of bone marrow stromal cells
Background: Bone marrow stromal cells (BMSCs) have an necessary utility prospect within the area of cell remedy for varied neurodegenerative ailments, and inducing components that regulate BMSC differentiation are proposed as a promising therapeutic technique. On this examine, we explored the impact of glial cell-derived neurotrophic issue (GDNF) on the course of BMSC differentiation.
Strategies: BMSCs have been remoted from rat bone marrow and induced by GDNF. The results of GDNF on BMSC viability and proliferation have been verified by cell counting equipment-8, MTT, bromodeoxyuridine, and move cytometry assays. Neuronal differentiation from BMSCs was detected by quantitative real-time polymerase chain response and immunofluorescence through measuring the expression of a number of neural particular markers.
Outcomes: In comparison with untreated BMSCs, GDNF induced the differentiation of BMSCs into neuron-like cells and enhanced the expression ranges of neuronal markers together with nestin and NCAM. Furthermore, the expression of SCF was suppressed by GDNF stimulation.
Conclusion: GDNF may elevate the differentiation of BMSCs into neuron-like cells and may very well be thought-about as an efficient candidate cell for future neuroscience analysis.
Key phrases: Alzheimer’s illness; bone marrow stromal cells; cell remedy; glial cell-derived neurotrophic issue; neuronal differentiation.

Molecular detection of Mycobacterium tuberculosis from buccal swabs amongst grownup in Peru

Tuberculosis (TB) prognosis depends on a sputum pattern, which can’t be simply obtained from all symptomatic sufferers. Mycobacterium tuberculosis DNA will be detected from oral swabs, a noninvasive, protected different pattern sort; nonetheless, reported sensitivities have been variable and sure rely upon pattern assortment, processing procedures and host traits. We analyzed three buccal swab samples from 123 adults with culture-confirmed TB in Lima, Peru. We in contrast the sensitivity and specificity of two pattern assortment units (OmniSwab and EasiCollect FTA playing cards) and examined components related to detection. DNA was extracted with a commercially out there equipment and detected through real-time PCR IS6110 amplification.
General sensitivity for buccal samples was 51% (95% Confidence Interval [CI] 42-60%). Specificity from a single pattern amongst wholesome controls was 96.7% (95% CI 83-99.9%). Optimistic sputum smear and cavitary illness, correlates of illness burden, have been related to detection through buccal swab. Though we noticed greater sensitivities with the Omniswab samples, this gave the impression to be due primarily to variations in affected person traits (e.g., cavitary illness). General, our findings assist the potential for a buccal sample-based TB assay. Future work ought to concentrate on assay optimization and streamlining the assay workflow.
Our outcomes confirmed that SSa pretreatment may considerably scale back mind harm, enhance neurological operate restoration, and reduce the water content material of mind tissues in comparison with the mannequin group. SSa pretreatment considerably lowered the serum HMGB1 degree and downregulated the contents of inflammatory cytokines together with tumor necrosis factor-α, interleukin-1 beta, and interleukin-6. Moreover, SSa pretreatment may attenuate the decreased TLR4 and nucleus NF-κB within the mind of MCAO rats. The protein degree of HMGB1 within the nucleus was considerably upregulated within the SSa pretreatment group.
We ready CDDP/CQ-PLA NPs and CDDP-PLA NPs. Transmission electron microscope (TEM) and dynamic gentle scattering (DLS) have been used to detect the physiological traits and particle dimension parameters of drug-loaded nanoparticles. The drug focus and cumulative launch have been measured by UV and visual spectrophotometer. MTT assay was used to detect viability of Cal-27 cells. Annexin/PI staining was used to detect cell apoptosis. Organic kits have been used to detect malondialdehyde (MDA) content material, catalase (CAT) exercise, antioxidant enzyme superoxide dismutase (SOD) exercise and glutathione peroxidase (GSH PX) exercise in Cal-27 cells. Western blot was used to detect apoptosis and autophagy of Cal-27 cells.
 Glial cell induced neural differentiation of bone marrow stromal cells

Glial cell induced neural differentiation of bone marrow stromal cells

Salivary Carbohydrate-Poor Transferrin in Alcohol- and Nicotine-Dependent Males

Serum carbohydrate-deficient transferrin (CDT), an 80 kDa glycoprotein, is among the mostly employed biomarkers to detect alcohol dependence. Some salivary glycoproteins comparable to α-amylase, clusterin, haptoglobin, gentle/heavy-chain immunoglobulin, and transferrin, which alter glycosylation in alcohol-dependent individuals, have been recommended to be potential alcohol markers. Nevertheless, their identification is predicated on oblique evaluation of lectin glycosidic bonds and molecular weight. We investigated the CDT content material within the saliva of alcohol- and nicotine-dependent males.
The CDT focus (ng/mL, ng/mg protein) was decided by an Enzyme-Linked Immunosorbent Assay (ELISA) industrial equipment in 55 males: 20 wholesome social drinkers (C), 10 power cigarette people who smoke (S), 10 alcohol-dependent non-smokers (A), and 15 alcohol-dependent people who smoke (AS). Surprisingly, there have been no variations within the concentrations of CDT between the studied teams. Salivary pH was the bottom within the AS and the best within the A gaggle. Subsequently, salivary CDT can’t be used as an alcohol dependence marker as measured by ELISA. We recommend that direct identification of glycoproteins is important to seek for potential salivary alcohol biomarkers. Molecules smaller than 40 kDa, which simply translocate from blood to the saliva, could be most popular as salivary alcohol markers.

TAGAP Recombinant Protein (Human) (Recombinant- Tag)

RP030880 100 ug Ask for price

TAGLN Recombinant Protein (Human) (Recombinant- Tag)

RP030883 100 ug Ask for price

TAGLN Recombinant Protein (Human) (Recombinant- Tag)

RP030886 100 ug Ask for price

TAGLN2 Recombinant Protein (Human) (Recombinant Tag)

RP030889 100 ug Ask for price

TAGLN2 Recombinant Protein (Human) (Recombinant Tag)

RP030892 100 ug Ask for price

TAGLN3 Recombinant Protein (Human) (Recombinant Tag)

RP030895 100 ug Ask for price

CTAG1A Recombinant Protein (Human) (Recombinant- Tag)

RP038332 100 ug Ask for price

CTAG1B Recombinant Protein (Human) (Recombinant- Tag)

RP038335 100 ug Ask for price

CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)

RP038338 100 ug Ask for price

TAGAP Recombinant Protein (Human) (Recombinant- Tag)

RP043930 100 ug Ask for price

CTAGE1 Recombinant Protein (Human) (Recombinant- Tag)

RP008266 100 ug Ask for price

CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)

RP008269 100 ug Ask for price

CTAGE6P Recombinant Protein (Human) (Recombinant Tag)

RP008272 100 ug Ask for price

CTAGEP Recombinant Protein (Human) (Recombinant- Tag)

RP008275 100 ug Ask for price

TAGLN Recombinant Protein (Rat) (Recombinant- Tag)

RP232205 100 ug Ask for price

TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)

RP232208 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)

RP232211 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)

RP232214 100 ug Ask for price

CTAGE3P Recombinant Protein (Human) (Recombinant Tag)

RP053400 100 ug Ask for price

CTAGE4 Recombinant Protein (Human) (Recombinant- Tag)

RP053403 100 ug Ask for price

CTAGE8 Recombinant Protein (Human) (Recombinant- Tag)

RP053409 100 ug Ask for price

CTAGE9 Recombinant Protein (Human) (Recombinant- Tag)

RP053412 100 ug Ask for price

TAGAP Recombinant Protein (Mouse) (Recombinant- Tag)

RP177155 100 ug Ask for price

Tagap1 Recombinant Protein (Mouse) (Recombinant Tag)

RP177158 100 ug Ask for price

TAGLN Recombinant Protein (Mouse) (Recombinant- Tag)

RP177161 100 ug Ask for price

TAGLN2 Recombinant Protein (Mouse) (Recombinant Tag)

RP177164 100 ug Ask for price

TAGLN3 Recombinant Protein (Mouse) (Recombinant Tag)

RP177167 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126449 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126452 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126455 100 ug Ask for price

Recombinant Batroxobin

7-04504 10µg Ask for price

Recombinant Batroxobin

7-04505 50µg Ask for price

Recombinant Batroxobin

7-04506 1mg Ask for price

Recombinant Hirudin

7-05344 2µg Ask for price

Recombinant Hirudin

7-05345 10µg Ask for price

Recombinant Hirudin

7-05346 1mg Ask for price

Recombinant Streptavidin

7-06553 5mg Ask for price

Recombinant Streptavidin

7-06554 20mg Ask for price

Recombinant Streptavidin

7-06555 1gr Ask for price

Recombinant Hantavirus

7-07429 100µg Ask for price

Recombinant Hantavirus

7-07430 500µg Ask for price

Recombinant Hantavirus

7-07431 1000µg Ask for price

Recombinant Lysostaphin

7-03085 1mg Ask for price

Recombinant Lysostaphin

7-03086 5mg Ask for price

Recombinant Lysostaphin

7-03087 10mg Ask for price

Recombinant Protease

7-03484 50IU Ask for price

Recombinant Protease

7-03485 150IU Ask for price

Recombinant Protease

7-03486 1,000IU Ask for price

Recombinant Staphylokinase

7-03598 20µg Ask for price

Recombinant Staphylokinase

7-03599 100µg Ask for price

Recombinant Staphylokinase

7-03600 1mg Ask for price

Recombinant Streptokinase

7-03601 250,000IU Ask for price

Recombinant Streptokinase

7-03602 750,000IU Ask for price

Recombinant Streptokinase

7-03603 1.5MIU Ask for price

Recombinant Urease

7-03790 1mg Ask for price

Recombinant Urease

7-03791 5mg Ask for price

Recombinant Urease

7-03792 50mg Ask for price

Recombinant LukAB

0510-001 100ug
EUR 486
Description:
  • Related to: Staphylococcus
  • Applications: ELISA, WB

Ribokinase (Recombinant)

20-abx072011
  • EUR 393.60
  • EUR 8043.60
  • EUR 1095.60
  • 10 ug
  • 1 mg
  • 90 µg

Ribokinase (Recombinant)

20-abx072012
  • EUR 393.60
  • EUR 7860.00
  • EUR 1095.60
  • 10 ug
  • 1 mg
  • 90 µg

Lysostaphin (Recombinant)

20-abx073003
  • EUR 710.40
  • EUR 276.00
  • EUR 493.20
  • 10 mg
  • 1 mg
  • 5 mg

Streptokinase (Recombinant)

abx073012-15MIU 1.5M IU
EUR 1395.6

Streptokinase (Recombinant)

abx073012-250000IU 250.000 IU
EUR 526.8

Streptokinase (Recombinant)

abx073012-750000IU 750.000 IU
EUR 861.6

NAT6 (Recombinant)

20-abx073034
  • EUR 727.20
  • EUR 460.80
  • EUR 560.40
  • 10 ug
  • 2 µg
  • 5 ug

HTRA2 (Recombinant)

20-abx073047
  • EUR 276.00
  • EUR 2798.40
  • EUR 393.60
  • 10 ug
  • 1 mg
  • 50 ug

GALE (Recombinant)

20-abx073137
  • EUR 3433.20
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

AASDHPPT (Recombinant)

20-abx073160
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ADSL (Recombinant)

20-abx073250
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Cystathionase (Recombinant)

20-abx073257
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ACOT7 (Recombinant)

20-abx073258
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ACAA1 (Recombinant)

20-abx073272
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Transaldolase (Recombinant)

20-abx073275
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Gankyrin (Recombinant)

20-abx073293
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

Catalase (Recombinant)

20-abx073353
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ADPRH (Recombinant)

20-abx073354
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Carboxymethylenebutenolidase (Recombinant)

20-abx073357
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

PAFAH1B3 (Recombinant)

20-abx073360
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Renalase (Recombinant)

20-abx073367
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

SMUG1 (Recombinant)

20-abx073377
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

MPST (Recombinant)

20-abx073378
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

TDP1 (Recombinant)

20-abx073385
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

MMP10 (Recombinant)

20-abx073421
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 25 ug
  • 5 ug

MMP1 (Recombinant)

20-abx073422
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ADAL (Recombinant)

20-abx073435
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Klotho (Recombinant)

20-abx073512
  • EUR 4519.20
  • EUR 393.60
  • EUR 276.00
  • 0.5 mg
  • 20 ug
  • 5 ug

Protease (Recombinant)

abx073528-1000IU 1.000 IU
EUR 777.6

Protease (Recombinant)

abx073528-150IU 150 IU
EUR 393.6

Protease (Recombinant)

abx073528-50IU 50 IU
EUR 276

Latexin (Recombinant)

20-abx073558
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Aspartoacylase (Recombinant)

20-abx073571
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

NDUFAF1 (Recombinant)

20-abx073578
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ACY1 (Recombinant)

20-abx073654
  • EUR 393.60
  • EUR 7108.80
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

ADAT2 (Recombinant)

20-abx073661
  • EUR 393.60
  • EUR 7108.80
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

APEX1 (Recombinant)

20-abx073692
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

NDUFAF2 (Recombinant)

20-abx073714
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

ACAA2 (Recombinant)

20-abx073818
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

ADAT1 (Recombinant)

20-abx073911
  • EUR 393.60
  • EUR 9447.60
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Staphylokinase (Recombinant)

20-abx073914
  • EUR 393.60
  • EUR 1228.80
  • EUR 276.00
  • 100 ug
  • 1 mg
  • 20 ug

Urease (Recombinant)

20-abx073924
  • EUR 276.00
  • EUR 1612.80
  • EUR 393.60
  • 1 mg
  • 50 mg
  • 5 mg

TDO2 (Recombinant)

20-abx073952
  • EUR 276.00
  • EUR 1930.80
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

NDUFAF4 (Recombinant)

20-abx073976
  • EUR 276.00
  • EUR 1930.80
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

YOD1 (Recombinant)

20-abx073982
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Fibrillarin (Recombinant)

20-abx074008
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

TurboNuclease, Recombinant

9207-200KU Ask for price

TurboNuclease, Recombinant

9207-50KU
EUR 548.4

Uricase, Recombinant

DIA-294 5KU
EUR 453.6

Neuraminidase, recombinant

NATE-1683 3ku
EUR 712.8

Recombinant Enterokinase

SJE02-01 1000U/vial
EUR 459.6

Recombinant EGFP

STA-201 100 ?g
EUR 470.4
Description:
  • Recombinant EGFP contains a 6x-His tag at the C-terminus. Provided as 100 µg/vial at a concentration of 1 mg/mL.
  • Includes 6xHis-tag at C-terminus
  • Provided at 100 ?g/vial at 1.0 mg/mL in 1X PBS
  • Recombinant Protein purified from E. coli

Recombinant EGFP

STA-201-5 5 x 100 µg
EUR 1681.2
Description:
  • Recombinant EGFP contains a 6x-His tag at the C-terminus. Provided as 5 vials of 100 µg at a concentration of 1 mg/mL.
  • Includes 6xHis-tag at C-terminus
  • Provided at 100 ?g/vial at 1.0 mg/mL in 1X PBS
  • Recombinant Protein purified from E. coli

Recombinant RFP

STA-202 100 ?g
EUR 470.4
Description:
  • Recombinant RFP contains a 6x-His tag at the C-terminus. Provided as 100 µg/vial at a concentration of 1 mg/mL.
  • Includes 6xHis-tag at C-terminus
  • Provided at 100 ?g/vial at 1.0 mg/mL in 1X PBS
  • Recombinant Protein purified from E. coli

Recombinant RFP

STA-202-5 5 x 100 µg
EUR 1681.2
Description:
  • Recombinant RFP contains a 6x-His tag at the C-terminus. Provided as 5 vials of 100 µg at a concentration of 1 mg/mL.
  • Includes 6xHis-tag at C-terminus
  • Provided at 100 ?g/vial at 1.0 mg/mL in 1X PBS
  • Recombinant Protein purified from E. coli

Recombinant Human Apoliprotein-J (Clusterin Human Recombinant)

CC014-002 2ug
EUR 351.6

Recombinant Human Apoliprotein-J (Clusterin Human Recombinant)

CC014-010 10ug
EUR 489.6

Recombinant Human Apoliprotein-J (Clusterin Human Recombinant)

CC014-101 1mg
EUR 13344

Recombinant Rat Apoliprotein-J (Clusterin Rat Recombinant)

CC015-002 2ug
EUR 351.6

Recombinant Rat Apoliprotein-J (Clusterin Rat Recombinant)

CC015-010 10ug
EUR 489.6

Recombinant Rat Apoliprotein-J (Clusterin Rat Recombinant)

CC015-101 1mg
EUR 8322

CTAG2 Recombinant Protein (Rat) (Recombinant-P Tag)

RP196619 100 ug Ask for price

CTAG2 Recombinant Protein (Human) (Recombinant-P Tag)

RP053376 100 ug Ask for price

CTAG2 Recombinant Protein (Mouse) (Recombinant-P Tag)

RP126446 100 ug Ask for price

HSA Recombinant, Human Serum Albumin Recombinant Protein, Plant

PROTP02768-1 Regular: 50mg
EUR 565.2
Description: HSA Human Recombinant produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.The optimum concentration for recombinant Albumin to be used in cell culture ranges between 0.5gr to 2gr per liter3.;The recombinant Albumin is purified by proprietary chromatographic techniques.

HSA Recombinant, Human Serum Albumin Recombinant Protein, HEK

PROTP02768-2 Regular: 100ug
EUR 380.4
Description: Recombinant Human Serum Albumin produced in HEK cells is a glycosylated monomer, having a molecular weight range of 60-65kDa due to glycosylation.;The HSA is purified by proprietary chromatographic techniques.

Recombinant other HSA Recombinant Protein, Untagged, Rice-100mg

QP12317-100mg 100mg
EUR 675.6

Recombinant other HSA Recombinant Protein, Untagged, Rice-10mg

QP12317-10mg 10mg
EUR 195.6

Recombinant other HSA Recombinant Protein, Untagged, Rice-20ug

QP12317-20ug 20ug
EUR 186

Recombinant other HSA Recombinant Protein, Untagged, Rice-50mg

QP12317-50mg 50mg
EUR 435.6

Human Recombinant PTP1B

6301-100
EUR 398.4

Human Recombinant PIN1

6304-100
EUR 510

Human Recombinant Thioredoxin

6305-100
EUR 398.4

Human Recombinant UCHL1

6306-100
EUR 510

Human Recombinant PPIL1

6317-100
EUR 444

Human Recombinant PPIL2

6325-50
EUR 588

Human Recombinant PPIL3

6326-100
EUR 548.4

Human Recombinant PPIL4

6327-50
EUR 588

Human Recombinant TXNRD1

6330-100
EUR 548.4

Human Recombinant ALDH2

6332-100
EUR 510

Human Recombinant ALDH3A1

6333-50
EUR 588

Human Recombinant AKR7A3

6334-50
EUR 588

Human Recombinant AKR7A2

6335-100
EUR 590.4

Human Recombinant AKR1C1

6336-100
EUR 510

Human Recombinant AKR1C3

6337-100
EUR 568.8

Human Recombinant AKR1C3

6337-1000
EUR 2924.4

Human Recombinant AKR1C4

6338-100
EUR 548.4

AKR1B10, human recombinant

6339-100
EUR 548.4

AKR1B10, human recombinant

6339-1000
EUR 1918.8

Human Recombinant FKBP1B

6341-100
EUR 548.4

Human Recombinant FKBP3

6342-100
EUR 510

Human Recombinant FKBP4

6343-100
EUR 457.2

Human Recombinant FKBP6

6344-50
EUR 548.4

Human Recombinant FKBPL

6345-10
EUR 548.4

Human Recombinant GSTA1

6346-100
EUR 510
 KCNMB2-AS1 expression in NSCLC tissues and cells was detected utilizing reverse transcription-quantitative polymerase chain response. Cell proliferation, apoptosis, migration, and invasion have been evaluated utilizing Cell Counting Package-8, move cytometry, Transwell migration, and Transwell invasion assays, respectively. In vivo tumor xenograft fashions have been constructed to evaluate tumorigenicity. Bioinformatics predictions have been carried out to determine microRNAs concentrating on KCNMB2-AS1. Interactions between KCNMB2-AS1 and miR-374a-3p have been analyzed utilizing RNA immunoprecipitation, luciferase reporter, and rescue experiments.