Effect of Cryptotanshinone on Measures of Rat Cardiomyocyte Oxidative Stress and Gene Activation Associated with Apoptosis
Background: Oxidative stress is a key issue that ends in cardiomyocyte apoptosis and cardiovascular illnesses. Cryptotanshinone (CTS), one of many main bioactive constitutes extracted from the foundation of the plant Salvia miltiorrhizaBunge, has been extensively studied for numerous illness therapies. Nonetheless, the roles of CTS on cardiomyocytes stay unclear.
Strategies: Within the current research, neonatal rat cardiomyocytes had been pretreated with CTS for four h earlier than being uncovered to H2O2. Cell viability for the cells with or with out pretreatment with CTS earlier than publicity to H2O2 was evaluated by the MTT assay. Manufacturing of lactate dehydrogenase (LDH), nitric oxide (NO), prostaglandin E2 (PGE2), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxides (GSH-Px) was quantified by corresponding detection kits. The mRNA ranges of Bcl-2 antiapoptotic and Bax-like proapoptotic genes had been quantified with RT-PCR. Manufacturing of reactive oxygen species (ROS) was certified and quantified with a dichlorofluorescein diacetate mobile ROS detection assay equipment. The extracellular signal-related kinase (ERK) phosphorylation and nuclear issue κB (NF-κB) activation had been measured by Western blot.
Outcomes: Our outcomes revealed that the CTS pretreatment might improve cell viability and promote Bcl-2 antiapoptotic gene expression. Moreover, CTS might abolish the H2O2-induced manufacturing of NO, LDH, and PGE2. In step with these findings, CTS might inhibit ROS and MDA manufacturing and promote SOD, CAT, and GSH-Px actions. Mechanistically, CTS might obtain these processes by inhibiting ERK and NF-κB sign pathways.
Conclusion: CTS protects cardiomyocytes in opposition to the H2O2-induced mobile accidents by ERK and NF-κB inactivation and ROS scavenging. Subsequently, CTS is a promising reagent in opposition to ROS-induced cardiomyopathy.

Analysis of NGS-based approaches for SARS-CoV-2 complete genome characterisation

For the reason that starting of the COVID-19 outbreak, SARS-CoV-2 whole-genome sequencing (WGS) has been carried out at unprecedented fee worldwide with using very numerous Subsequent-Era Sequencing (NGS) strategies. Herein, we examine the efficiency of 4 NGS-based approaches for SARS-CoV-2 WGS. Twenty-four scientific respiratory samples with a big scale of Ct values (from 10.7 to 33.9) had been sequenced with 4 strategies. Three used Illumina sequencing: an in-house metagenomic NGS (mNGS) protocol and two newly commercialised kits together with a hybridisation seize technique developed by Illumina (DNA Prep with Enrichment equipment and Respiratory Virus Oligo Panel, RVOP), and an amplicon sequencing technique developed by Paragon Genomics (CleanPlex SARS-CoV-2 equipment).
We additionally evaluated the extensively used amplicon sequencing protocol developed by ARTIC Community and mixed with Oxford Nanopore Applied sciences (ONT) sequencing. All 4 strategies yielded near-complete genomes (>99%) for top viral masses samples (n = 8), with mNGS and RVOP producing essentially the most full genomes. For mid viral masses (Ct 20-25), amplicon-based enrichment strategies led to genome protection >99 per cent for all samples whereas 1/Eight pattern sequenced with RVOP and a couple of/Eight samples sequenced with mNGS had a genome protection beneath 99 per cent. For low viral masses (Ct ≥25), amplicon-based enrichment strategies had been essentially the most delicate methods. All strategies had been extremely concordant when it comes to identification in full consensus sequence.
Only one mismatch in three samples was noticed in CleanPlex vs the opposite strategies, because of the devoted bioinformatics pipeline setting a excessive threshold to name SNP in comparison with reference sequence. Importantly, all strategies accurately recognized a newly noticed 34nt-deletion in ORF6 however required particular bioinformatic validation for RVOP. Lastly, as a significant warning for focused methods, a lack of protection in any given area of the genome ought to alert to a possible rearrangement or a SNP in primer-annealing or probe-hybridizing areas and would require additional validation utilizing unbiased metagenomic sequencing.

 Effect of Cryptotanshinone on Measures of Rat Cardiomyocyte Oxidative Stress and Gene Activation Associated with Apoptosis

Effect of Cryptotanshinone on Measures of Rat Cardiomyocyte Oxidative Stress and Gene Activation Associated with Apoptosis

Serum metabolomics research of girls with completely different annual decline charges of anti-Müllerian hormone: an untargeted gasoline chromatography-mass spectrometry-based research

Examinequery: Which metabolites are related to various charges of ovarian growing old, measured as annual decline charges of anti-Müllerian hormone (AMH) concentrations?
Abstractreply: Increased serum concentrations of metabolites of phosphate, N-acetyl-d-glucosamine, branched chained amino acids (BCAAs), proline, urea and pyroglutamic acid had been related to increased odds of quick annual decline fee of AMH.
What is thought already: Age-related fee of ovarian follicular loss varies amongst ladies, and the components underlying such inter-individual variations are primarily unknown. The speed of ovarian growing old is clinically vital resulting from its results on each copy and well being of girls. Metabolomics, a worldwide investigation of metabolites in organic samples, offers a possibility to review metabolites or metabolic pathways in relation to a physiological/pathophysiological situation. To this point, no metabolomics research has been performed concerning the variations within the charges of ovarian follicular loss.
Examine design, measurement, period: This potential research was performed on 186 reproductive-aged ladies with common menstrual cycles and historical past of pure fertility, randomly chosen utilizing random case choice choice in SPSS from the Tehran Lipid and Glucose Examine.
Members/supplies, setting, strategies: AMH concentrations had been measured at baseline (1999-2001) and the fifth follow-up examination (2014-2017), after a median follow-up of 16 years, by immunoassay utilizing Gen II equipment. The annual decline fee of AMH was calculated by dividing the AMH decline fee by the follow-up period (%/yr). The ladies had been categorized based mostly on the tertiles of the annual decline charges. Untargeted metabolomics evaluation of the fasting-serum samples collected through the second follow-up examination cycle (2005-2008) was carried out utilizing gasoline chromatography-mass spectrometry. A mixture of univariate and multivariate approaches was used to research the associations between metabolites and the annual decline charges of AMH.

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Rabbit anti-EBOV NP pAb

0301-012 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against EBOV NP (detects EBOV NP in virus-like particles (VLP))

Rabbit anti-SUDV NP pAb

0302-012 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against SUDV NP (detects SUDV NP in virus-like particles (VLP))

Rabbit anti-MARV NP pAb

0303-012 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against MARV NP (detects MARV NP in virus-like particles (VLP))

Rabbit anti-(SEA, SEB, TSST-1) pAb

0311-001 500ug
EUR 300
Description: Rabbit polyclonal antibody broadly reactive to SEB, SEA, SEC and TSST-1

Rabbit anti-Hla pAb

04-0010 500ug
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Description: Rabbit polyclonal reactive to S. aureus alpha hemolysin

Rabbit Anti-CMV PAb

E40PA2058 50ul
EUR 395
Description: Available in various conjugation types.

Rabbit anti-LukA pAb

0316-001 100ug
EUR 300
Description: Protein A purified rabbit polyclonal antibody reactive to S. aureus LukA

Rabbit anti-LukB pAb

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Description: Protein A purified rabbit polyclonal antibody reactive to S. aureus LukB

Rabbit anti-Junin pAb

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Rabbit anti alpaca IgG rabbit pAb

E28PT7952 100μl
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E44H16517 100ul
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Anti-Fyn Rabbit pAb

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Rabbit anti-Hlg B pAb

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Description: Rabbit polyclonal antibody reactive to the S. aureus Hlg C with His tag

Rabbit anti V5-Tag pAb

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AE036 100 ul
EUR 2015

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Description: Affinity purified rabbit polyclonal antibody against EBOV GP (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-SUDV GP pAb

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EUR 450
Description: Affinity purified rabbit polyclonal antibody against SUDV GP (detects SUDV GP in virus-like particles (VLP) and recombinant SUDV GP without the transmembrane region)

Rabbit anti-MARV GP pAb

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EUR 450
Description: Affinity purified rabbit polyclonal antibody against MARV GP (detects MARV GP in virus-like particles (VLP) and recombinant MARV GP without the transmembrane region)

Rabbit anti-BDBV GP pAb

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EUR 450
Description: Affinity purified rabbit polyclonal antibody reactive to BDBV GP (detects recombinant BDBV GP without the transmembrane region (BDBV rGPdTM))

Rabbit anti-LASV GP pAb

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EUR 420
Description: Affinity purified rabbit polyclonal antibody reactive to Lassa virus GP

Rabbit anti-LASV V pAb

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Description: Affinity purified rabbit polyclonal antibody reactive to Lassa virus Z protein

Rabbit anti-ZIKA GP pAb

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Description: Affinity purified rabbit polyclonal antibody reactive to Zika virus E protein

Rabbit anti GST-Tag pAb

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EUR 255
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Rabbit anti GFP-Tag pAb

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EUR 255
Description: Available in various conjugation types.

Rabbit anti GST-Tag pAb

AE006 100 ul
EUR 143

Rabbit anti Myc-Tag pAb

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EUR 65

Rabbit anti GFP-Tag pAb

AE011 50 ul
EUR 107.25

Rabbit anti His-Tag pAb

AE068 50 ul
EUR 109.2

Rabbit anti-MARV VLP pAb

04-0005 500ug
EUR 240
Description: Affinity purified rabbit polyclonal antibody against MARV GP, NP, and VP40 (detects MARV GP, NP, and VP40 in virus-like particles (VLP))

Rabbit anti-SUDV sGP pAb

0302-030 100ug
EUR 425
Description: Affinity purifed rabbit polyclonal antibody reactive to SUDV Soluble glycoprotein (sGP)

Rabbit anti-RESTV GP pAb

0305-001 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody reactive to RESTV GP (detects recombinant RESTV GP without the transmembrane region (RESTV rGPdTM))

Rabbit anti-CHIKV E2 pAb

0306-001 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody reactive to CHIKV strain 181/25

Rabbit anti-ZIKA NS1 pAb

0308-002 100ug
EUR 425
Description: Affinity purified rabbit polyclonal antibody reactive to Zika virus Non-Structural protein 1

Rabbit anti-EBOV VLP pAb

0315-001 500ug
EUR 425
Description: Affinity purified rabbit polyclonal antibody against EBOV GP, NP, and VP40 (detects EBOV GP, NP, and VP40 in virus-like particles (VLP))

Rabbit anti-EBOV VP40 pAb

0301-010 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against EBOV VP40 (detects EBOV VP40 in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-LukS-PV pAb

04-0009 500ug
EUR 325
Description: Rabbit polyclonal reactive to S. aureus Panton Valentine Luk-S

Rabbit anti-EBOV VP35 pAb

0301-040 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against EBOV VP35 (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-EBOV VP24 pAb

0301-046 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against EBOV VP24 (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-EBOV VP24 pAb

0301-047 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against EBOV VP24 (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-EBOV VP30 pAb

0301-048 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against EBOV VP30 (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-SUDV VP40 pAb

0302-001 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against SUDV VP40 (detects SUDV VP40 in virus-like particles (VLP) and recombinant SUDV GP without the transmembrane region)

Rabbit anti-MARV VP40 pAb

0303-001 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody against MARV VP40 (detects MARV VP40 in virus-like particles (VLP) and recombinant MARV VP40 without the transmembrane region)

Rabbit anti-LukF-PV pAb

0312-002 500ug
EUR 300
Description: Rabbit polyclonal reactive to S. aureus Panton Valentine Luk-F

pAb rabbit anti-human EPO

CT282 0.5 mg
EUR 220.8

Rabbit anti DDDDK-Tag pAb

AE004 50 ul
EUR 195

Rabbit anti-EBOV GPΔmuc pAb

0301-038 500 ug
EUR 425
Description: Protein A/G purified rabbit polyclonal antibody against EBOV GP (antibody detects recombinant EBOV GP (modified) and GP expressed in virus-like particles (VLP))

Rabbit Anti-Rabies Virus PAb

E40PA2105 50ul
EUR 395
Description: Available in various conjugation types.

Anti-4E-BP1 Rabbit pAb

E2306002 100ul
EUR 225
Description: Available in various conjugation types.

Anti-LC3A/B Rabbit pAb

E2306019 100ul
EUR 225
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E2321013 100ul
EUR 225
Description: Available in various conjugation types.

Rabbit anti-Chikungunya (CHIKV) pAb

04-0008 500ug
EUR 240
Description: protein G purified rabbit polyclonal against Chikungunya strain 181/25 purified from antiserum

anti-HCV NS5a rabbit pAb

199-A-01mg 0,1 mg
EUR 321
Description: anti-HCV NS5a rabbit (polycnonal) Produced against a recombinant protein

anti-HCV NS5a rabbit pAb

199-A-1000ug 1000 ug
EUR 1539
Description: anti-HCV NS5a rabbit (polycnonal) Produced against a recombinant protein

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266-A-01mg 0,1 mg
EUR 321
Description: anti-HCV NS5b rabbit (polycnonal)Produced against a recombinant protein

anti-HCV NS5b rabbit pAb

266-A-1000ug 1000 ug
EUR 1539
Description: anti-HCV NS5b rabbit (polycnonal)Produced against a recombinant protein

Anti-HA-tag Rabbit pAb

E2301113 100ul
EUR 225
Description: Available in various conjugation types.

Anti-hnRNP M Rabbit pAb

E2321003 100ul
EUR 225
Description: Available in various conjugation types.

Rabbit anti-LukAB pAb (neutralizing)

0313-001 500ug
EUR 350
Description: Protein A purified neutralizing rabbit polyclonal antibody reactive to S. aureus LukA and LukB

Anti-GST-tag Rabbit pAb

E2300195 100ul
EUR 225
Description: Available in various conjugation types.

Anti-MBP-tag Rabbit pAb

E2300462 100ul
EUR 225
Description: Available in various conjugation types.

Anti-Human IgG Rabbit pAb

E2301068 100ul
EUR 225
Description: Available in various conjugation types.

Rabbit anti-EEEV E2 GP pAb

0318-001 100ug
EUR 425
Description: Affinity purified rabbit polyclonal antibody reactive to Eastern Equine Encephalitis E2 glycoprotein

Rabbit anti-WEEV E2 GP pAb

0319-001 100ug
EUR 425
Description: Affinity purified rabbit polyclonal antibody reactive to Western Equine Encephalitis E2 glycoprotein

pAb rabbit anti-rat IL-4

CT058 0.5 mg
EUR 220.8

Rabbit Anti-Japanese Encephalitis PAb

E40PA2064 50ul
EUR 395
Description: Available in various conjugation types.

Anti-Caspase-3 Rabbit pAb

E2300968 100ul
EUR 225
Description: Available in various conjugation types.

pAb rabbit anti-rat IFN-β

CT024 0.5 mg
EUR 220.8

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Anti-EGF Receptor Rabbit pAb

E2321014 100ul
EUR 225
Description: Available in various conjugation types.

pAb rabbit anti-rat IFN-α1

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pAb rabbit anti-human IL-2

CT261 0.5 mg
EUR 312

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Rabbit anti-SARS-CoV2 RBD pAb

0373-001 100 ug
EUR 325
Description: Affinity-purified rabbit polyclonal antibody reactive to SARS-CoV-2 Spike protein receptor binding domain

Rabbit anti-CCHFV N protein pAb

04-0011 100ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody reactive to Crimean Congo Hemmoragic Fever N protein.

Rabbit anti-CCHFV Gc protein pAb

04-0012 100 ug
EUR 450
Description: Affinity purified rabbit polyclonal antibody reactive to Crimean Congo Hemmoragic Fever Gc protein.

pAb rabbit anti-human IL-12p70

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Rabbit Recombinant Anti-HBsAg PAb

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EUR 395
Description: Available in various conjugation types.

Rabbit anti-EBOV L-Polymerase pAb

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Description: Affinity purified rabbit polyclonal antibody against EBOV L-Polymerase (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit Anti-Enterovirus Type 71 PAb

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EUR 395
Description: Available in various conjugation types.

Rabbit Polyclonal Anti-NP Antibody

TA346671 100 µl Ask for price

Rabbit anti-EBOV GP pAb Biotinylated

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Description: Affinity purified rabbit polyclonal antibody against EBOV GP Biotinylated (detects EBOV GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region)

Rabbit anti-MARV GP pAb Biotinylated

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Description: Affinity purified rabbit polyclonal antibody against MARV GP Biotinylated (detects MARV GP in virus-like particles (VLP) and recombinant MARV GP without the transmembrane region)

Rabbit Anti-CMV PAb, HRP-conjugated

E40TA2239 50ul
EUR 395
Description: Available in various conjugation types.

Rabbit Anti-Coxsackievirus Type A16 PAb

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EUR 395
Description: Available in various conjugation types.

Rabbit Anti-Human Immunoglobulin E PAb

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Anti-Laminin γ-2 Rabbit pAb

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EUR 369.6

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Anti-GFP/eGFP-tag Rabbit pAb

E2300943 100ul
EUR 225
Description: Available in various conjugation types.

Rabbit Anti-LCMV NP Polyclonal antibody

CABT-CS971 100 μl
EUR 720
Description: Rabbit

Rabbit Anti-Human PTK2B PAb (aa 400~404)

CPB-1099RH 101ul
EUR 552

Anti-APG1 (ULK1) (Center) Rabbit pAb

E2616611 100μg/100μl
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Rabbit anti-SARS-CoV2 S1-C pAb

0371-001 100 ug
EUR 325
Description: Affinity-purified rabbit polyclonal antibody reactive to SARS-CoV-2 Spike protein S1 subunit C-terminus

Rabbit anti-SARS-CoV2 S1-N pAb

0372-001 100 ug
EUR 325
Description: Affinity-purified rabbit polyclonal antibody reactive to SARS-CoV-2 Spike protein S1 subunit N-terminus

Rabbit anti-SARS-CoV2 S2-C pAb

0374-001 100 ug
EUR 325
Description: Affinity-purified rabbit polyclonal antibody reactive to SARS-CoV-2 Spike protein S2 subunit C-terminus

Rabbit anti-EBOV Soluble GP (sGP) pAb

0365-001 100ug
EUR 450
Description: Affinity purifed rabbit polyclonal antibody reactive to EBOV Soluble glycoprotein (sGP)

Anti-14-3-3(pan) Rabbit pAb

E2306001 100ul
EUR 225
Description: Available in various conjugation types.

pAb rabbit anti-human IL-4 receptor

CT248 0.5 mg
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Rabbit Anti-Vero Cell Protein (HCP) PAb

E40PA2109 50ul
EUR 395
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Rabbit anti-Rift Valley Fever (RVFV) pAb

04-0001 500ug
EUR 308.4
Description:
  • Related to: Other Viruses
  • Applications: ELISA, WB

Rabbit Anti-Hepatitis A Virus (HAV) PAb

E40PA2063 50ul
EUR 395
Description: Available in various conjugation types.

Anti-Phospho-Akt (Thr308) Rabbit pAb

E2321006 100ul
EUR 225
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Anti-Phospho-Chk2 (Thr68) Rabbit pAb

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Anti-Phospho-CDK2 (Thr160) Rabbit pAb

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Rabbit Anti-Sabin Poliovirus Type I PAb

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EUR 395
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Rabbit Anti-Sabin Poliovirus Type II PAb

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Rabbit Anti-Goat IgG(H+L) pAb, Cy3

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Rabbit Anti-Goat IgG(H+L) pAb, HRP

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Rabbit Anti-Goat IgG(H+L) pAb, FITC

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Rabbit Inactivated Anti-Pertactin (PRN) PAb

E40PA2065 50ul
EUR 395
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Rabbit Anti-Rabies Virus PAb, HRP-conjugated

E40TA2234 50ul
EUR 395
Description: Available in various conjugation types.

Rabbit Anti-Chicken IgG(H+L) pAb, HRP

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EUR 93.33

Rabbit Anti-Candida albicans Proteinase K PAb

E40PA2104 50ul
EUR 395
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Rabbit Inactivated Anti-Pertussis Toxiod PAb

E40PA2066 50ul
EUR 395
Description: Available in various conjugation types.

anti-HCV NS5a rabbit pAb genotype 2A

276-A-01mg 0,1 mg
EUR 321
Description: anti-Rotavirus p42 IgG2a (monoclonal) Produced against purified bovine rotavirus virus.
Importantoutcomes and the position of probability: After adjusting the baseline values of age, AMH and BMI, 29 metabolites had been positively correlated with the annual AMH decline charges. The comparisons among the many tertiles of the annual decline fee of AMH revealed a rise within the relative abundance of 15 metabolites within the ladies with a quick decline (tertile 3), in comparison with these with a sluggish decline (tertile 1). There was no distinct separation between ladies with sluggish and quick decline charges whereas contemplating 41 metabolites concurrently utilizing the principal element evaluation and the partial least-squares discriminant evaluation fashions. The chances of quick AMH decline was elevated with increased serum metabolites of phosphate, N-acetyl-d-glucosamine, BCAAs, proline, urea and pyroglutamic acid. Amino sugar and nucleotide sugar metabolism, BCAAs metabolism and aminoacyl tRNA biosynthesis had been among the many most vital pathways related to the quick decline fee of AMH.